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  cold ischemic injury
    Conclusions The tempera- ture of 4℃ is appropriate for hypothermic liver preservation in rats for relieve the cold ischemic injury.
    结论 4℃对于大鼠肝脏是最适保存温度,它能够明显减轻大鼠肝脏的冷损伤程度。
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    Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.
    结论4℃对大鼠胰腺是最适保存温度,它能够明显减轻大鼠胰腺的冷损伤程度。
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    The study shows that continuous oxygenated warm blood cardioplegia perfusion coronary artery is a safty,effective method,which can prevent clod injury and ischemic reperfusion damage.
    本实验说明持续灌注氧合心麻液是一种安全、有效的心肌保护方法,可避免心脏冷损伤及缺血心肌再灌注损伤。
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We have observed the effect of oxygenated warm biood cardiopiegia induction heart ar- rest on myocardoal ultrastructure and analysed clinical result in ten patients who suffered from extra- corporal circulatio operation.Electron microscope result shows: Oxygented warm blood cardioplegia continuous perfusion coronary artery may protect protect the ultrastracture of myocardium,it show- that mitochondria arrange keeps in good order,mitochondria is swollen and hydropic, the membrane of mitochondria is clear.Myofibril...

We have observed the effect of oxygenated warm biood cardiopiegia induction heart ar- rest on myocardoal ultrastructure and analysed clinical result in ten patients who suffered from extra- corporal circulatio operation.Electron microscope result shows: Oxygented warm blood cardioplegia continuous perfusion coronary artery may protect protect the ultrastracture of myocardium,it show- that mitochondria arrange keeps in good order,mitochondria is swollen and hydropic, the membrane of mitochondria is clear.Myofibril keeps in good order.Myofidril is not break off and liquid.Nucleus and chromatin is normal.Endoplasmic reticulum is not dilatation.After operation ten patients recover nor- mal sinus ryhthm.Time of empty beating is very short,cardiac function is very good.There is not an complication. The study shows that continuous oxygenated warm blood cardioplegia perfusion coronary artery is a safty,effective method,which can prevent clod injury and ischemic reperfusion damage.

我们对10例体外循环手术病例采用氧合温血心麻液持续灌注心脏停跳,观察其对心肌超微结构的影响并分析了临床结果。电镜结果表明采用氧合温血持续灌注可保持心肌超微结构正常,镜下线粒体排列整齐,线粒体内、外膜清晰可辨,未见明显线粒体肿胀及空泡现象,肌原纤维走向规则,明暗带规整.未发现肌纤维断裂液化现象,细胞核染色质均勺,核膜清楚,未发现肌浆网扩张现象。所有病例均自动复跳,空跳时间明显缩短,复跳后心功能良好,术后血流动力学恢复满意,无任何并发症。本实验说明持续灌注氧合心麻液是一种安全、有效的心肌保护方法,可避免心脏冷损伤及缺血心肌再灌注损伤。

Objective To investigate the storage temperature on liver function to determine the proximal storage temperature for liver preservation.Methods Cold liver preservation and orthotopic liver transplantation (OLT)were performed in rats.The liver was preserved for 6,18 and 30 hours in UW solution at specific tempera- tures(0℃,4℃,8℃ and 12℃).OLT was performed after the preservation for 18 hours.Liver function was de- termined by measurement of AST,LDH,ATP and EC.Meanwhile,the liver was observed with microscopy.Re-...

Objective To investigate the storage temperature on liver function to determine the proximal storage temperature for liver preservation.Methods Cold liver preservation and orthotopic liver transplantation (OLT)were performed in rats.The liver was preserved for 6,18 and 30 hours in UW solution at specific tempera- tures(0℃,4℃,8℃ and 12℃).OLT was performed after the preservation for 18 hours.Liver function was de- termined by measurement of AST,LDH,ATP and EC.Meanwhile,the liver was observed with microscopy.Re- sults 1)The levels of AST and LDH were 4℃<0℃<8℃<12℃ at the 18th and 30th hour after the preserva- tion and at the 2nd hour after the transplantation(P<0.01 or 0.05).2) The levels of ATP and EC were 4℃> 0℃>8℃>12℃ at the 18th and 30th hour after preservation and at the 20th min after transplantation(P<0.01 or 0.05).3)The histological damage was slighter in 4℃ group than in other groups.Conclusions The tempera- ture of 4℃ is appropriate for hypothermic liver preservation in rats for relieve the cold ischemic injury.

目的研究温度对大鼠肝脏功能的影响,以确定大鼠肝脏的最适保存温度。方法采用单纯低温保存方法及建立大鼠原位肝移植模型研究保存温度对大鼠肝脏功能的影响。实验对0℃、4℃、8℃、12℃进行研究。保存时限为6,18及30h,保存18h 后行肝移植术。检测指标包括肝脏酶学、能量指标及组织形态学改变。结果①保存18,30h 的灌出液及移植后2h 血清中 AST、LDH 水平为4℃<0℃<8℃<12℃(P<0.01或P<0.05)。②保存18、30h 及移植后20min 的肝脏能量指标ATP、EC 为4℃>0℃>8℃>12℃(P<0.01或P<0.05)。③肝脏形态学示4℃组损害较其它组轻。结论 4℃对于大鼠肝脏是最适保存温度,它能够明显减轻大鼠肝脏的冷损伤程度。

Objective To investigate the effects of preservative temperature on pancreatic function and determine the optimal preservative temperature for pancreatic transplantation. Methods Cold pancreatic preservation was performed and a homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was established. The pancreas was preserved for 6 h in UW solution at specific temperatures(0 ℃, 4 ℃, 8 ℃ and (12 ℃)). After preservation, pancreatic tissue was taken for histologic examination in...

Objective To investigate the effects of preservative temperature on pancreatic function and determine the optimal preservative temperature for pancreatic transplantation. Methods Cold pancreatic preservation was performed and a homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was established. The pancreas was preserved for 6 h in UW solution at specific temperatures(0 ℃, 4 ℃, 8 ℃ and (12 ℃)). After preservation, pancreatic tissue was taken for histologic examination in every group. ATP and total adenine nucleotides (TAN) were determined by using high performance liquid chromatography (HPLC). Blood glucose(BG), serum amylase and lipase were measured 24 h after transplantation. And the activity of myeloperoxidase (MPO) in the pancreatic grafts was also measured at the same time. Besides, histological observation was performed. Results Microscopic studies showed that the histomorphological changes of pancreas in 4 ℃ group were less obvious than those in other groups. Tissual concentrations of ATP and TAN decreased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group after 6 h of preservation(P<0.05). The levels of BG, serum lipase and MPO increased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group(P<0.05). The activity of MPO in 4 ℃ group (1.19±0.16 U/g )was significantly lower than that of the control group(0.26±0.09 U/g,P<0.05). Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.

目的探讨保存温度对移植胰腺功能的影响,确定大鼠胰腺的最适保存温度。方法采用原位灌注、单纯低温保存及建立同系大鼠异位全胰十二指肠移植模型,按不同保存温度(0℃、4℃、8℃、12℃)进行分组,保存6h后进行移植,通过光镜观察胰腺组织结构变化,高压液相色谱法检测保存6h后移植物ATP和总腺苷核苷酸(TAN)含量。移植24h后,检测血糖、血清中脂肪酶及淀粉酶含量,测定移植胰腺组织中髓过氧化酶(MPO)活性,并进行组织学观察。结果4℃组保存6h后和移植24h后的胰腺组织结构损伤明显轻于其它组。保存6h后胰腺组织中ATP和TAN水平在4℃组、0℃组、8℃组和12℃组依次降低,差异有显著性意义(P<0.05)。移植24h后血糖、血清脂肪酶及胰腺组织中MPO水平在4℃组、0℃组、8℃组和12℃组依次增高,差异均有显著性意义(P<0.05)。结论4℃对大鼠胰腺是最适保存温度,它能够明显减轻大鼠胰腺的冷损伤程度。

 
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