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发酵条件下
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    THE COMPARISON OF VARIOUS STRAINS OF AUREOBASIDIUM PULLULANS PRODUCING PULIULA AT DIFFERENT CONDITIONS
    几株出芽短梗霉在不同发酵条件下产生多糖的比较
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    Under the optimal fermentation conditions,the activity of fibrinolysin reached 3643 U/ml(urokinase unit) in shake flask,and 2050 U/ml(urokinase unit) in 15 L fermentor.
    在最适发酵条件下,摇瓶发酵产豆豉溶栓酶酶活达到3643U/mL发酵液(尿激酶单位),15L发酵罐发酵产豆豉溶栓酶酶活达到2050U/mL发酵液(尿激酶单位)。
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    When fermentations were conducted in anaerobic bottles,the final succinic acid concentration of SF-9(35.09 g/L) increased 21.4%,and the mass ratio of succinic acid/acetic acid increased from 3.3 to 7.5 compared with those of the parent strain.
    以50 g/L的葡萄糖为碳源,在培养瓶厌氧发酵条件下其琥珀酸产量(35.09 g/L)比出发菌株(28.91 g/L)提高了21.4%,琥珀酸/乙酸比率(w/w)从3.3∶1提高到7.5∶1。
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    In the same measure , the biomass was 10.25 mg/L, iron content of the yeast cell was 7.43 mg/g.
    利用60Co辐射诱变,选育出一株正突变菌株R4-5-27,在同等发酵条件下,生物量达到10.25mg/L,铁含量7.43mg/g,总铁含量为76.16mg/L,比出发菌株提高了1.59倍。
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    The enzyme activity reached 22.37U/ml in fermentation. When the L-asparaginase was treated by chemical precipitation process with ammonium sulfate,30.8% yield of enzyme with a specific activity of approximately 0.55U/mg was obtained and the purity of product was increased 9.85 fold.
    在此发酵条件下,L-天冬酰胺酶发酵液酶活力的峰值可达到22.37 U/ml、产酶稳定期持续7~8 d,利用吴氏提取工艺可以得到回收率为30.8%,纯化倍数为9.85,比活力为0.55 U/mg的L-天冬酰胺酶。
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We isolated a mutant 311-60 of Strep-tomyces hygroscopicus var. jinggangensis which was showed resistive to phosphate and produced more Jingsimycin when the parent strain was treated by UV and added

通过紫外线处理和高浓度磷酸盐选择分离到静丝霉素的耐磷高产突变株311-60,在10到30mM磷酸盐条件下抗生素的积累明显地超过了亲本菌株,在正常发酵条件下超产可达45.2%: 突变株203-4的菌落形态与亲本菌株差异较大,在10到30mM磷酸盐条件下其静丝霉素产量超过亲本,亦属于耐磷突变株。

In order to produce the polysaccharide of Schizophyllum commune Fr. by submerged culture technique, the suitable medium, optimal time and high-yielding strain producing polysaccharide were studied. 3 strains of S. commune which produce polysaccharide from fruit bodies of S. commune were isolated from Lingo temple and campus of Nanjing University and they were designated as Nanda 835, Nanda 843 and Nanda 853. The yields of polysaccharide and mycelium of Nanda 843 were determined and were compared by submerged...

In order to produce the polysaccharide of Schizophyllum commune Fr. by submerged culture technique, the suitable medium, optimal time and high-yielding strain producing polysaccharide were studied. 3 strains of S. commune which produce polysaccharide from fruit bodies of S. commune were isolated from Lingo temple and campus of Nanjing University and they were designated as Nanda 835, Nanda 843 and Nanda 853. The yields of polysaccharide and mycelium of Nanda 843 were determined and were compared by submerged culture in six different media. The experimental results indicated that he Soybean meal glucose liquid medium was found to be the preferable medium for polysaccharide synthesis by S. commune as compared to the other media. In such a medium, a dense suspension of S. commune with numerous white homogeneous mycelial pellets and abundant polysaccharide could be successfully produced. The medium composition is as following (g/l): glucose 30, soybean meal 5, yeast extract 2, KH_2PO_4 1, MgSO_4.7H_2O0.5 pH 5.5. The following optimal conditions were varified. pH 5-5.5; temperature 26-28℃;shake rate: 100-110 times/min. When pH value dropped to 4.9-4.7 residual sugar 1%, usually being 5-6 days. The white, fibrous polysaccharide could be precipitated from concentrative filtrate of 6 days-old cultures by the addition of equal volume 95% alcohol. Among the 3 strains tested it was found that Nanda 853 remarkably increase the yields of polysaccharide and produced about 5.5-6g/l in culture fluid of 6 days. The polysaccharide produced by Nanda 843 and Nanda 835 strains amount to 5 g/l and 2.8g/l respectively.

为了深层培养裂褶菌Schizophyllum commune Fr.产生多糖,对产多糖的适宜培养基,最佳时间,高产菌株进行了研究。从南京灵谷寺及南京大学校园生长的裂褶菌子实体分离到3株产多糖的裂褶菌菌株,编号南大835,南大843,南大853。对南大843用6种不同培养基进行深层培养,测定和比较了多糖和菌丝产量,其结果表明黄豆粉葡萄糖液体培养基是适于裂褶菌合成多糖的培养基,能培养出密集、白色、均匀的菌球和丰富的多糖。其组成为(g/L):葡萄糖30,黄豆粉5,酵母膏2,KH_2PO_4 1,MgSO_4·7H_2O0.5。pH5.5。最适发酵条件:pH5—5.5,温度26—28℃,振速:100—110次/分,当pH降至4.9—4.7,残糖量在1%以下,5—6天可终止发酵。在培养6天的浓缩滤液中加入等体积的95%乙醇后大量白色粘稠、纤维状的多糖被沉淀下来。在上述发酵条件下,3个菌株比较结果,南大853能明显提高多糖产量,6天的培养液中多糖量可达5.5—6g/L,南大843和南大835分别是5g/L和2.8g/L。

The studies on fermentation of Bacillus thuringiensis showed that thefermentation beer produced by 7 strains of this bacterium grown in onefermentation medium and the fermentation beer produced by 1 strain ofthis bacterium grown in seven fermentation media represented differenttoxicities against the test insect.The toxicity against the test insect wasrelated to the sensitivity of insect and to different strains of Bacillus thu-ringiensis,and also to the composition of fermentation media.Howeverthe toxicity was...

The studies on fermentation of Bacillus thuringiensis showed that thefermentation beer produced by 7 strains of this bacterium grown in onefermentation medium and the fermentation beer produced by 1 strain ofthis bacterium grown in seven fermentation media represented differenttoxicities against the test insect.The toxicity against the test insect wasrelated to the sensitivity of insect and to different strains of Bacillus thu-ringiensis,and also to the composition of fermentation media.Howeverthe toxicity was not correlated with spore count. Varies of N.C and C/N of media during fermentation process did notrelate to the toxicity of fermentation production against the test insect.Therefore,the potency of the fermentation production of Bacillus thuringi-ensis can be determined only by bioassay.

研究表明,苏云金杆菌(Bacillus thuringiensis)对马尾松毛虫(Dendrolimuspunctatus)幼虫的毒性与苏云金杆菌的不同亚种,同一亚种的不同菌株以及发酵培养基组分有相关性,而在发酵过程中培养基全氮、有机碳、碳氮比例的消长规律与对昆虫的毒性未见一定的相关性。在发酵过程中,同一菌株在同一培养基中的菌数与试虫毒性成正相关;而不同菌株在同一培养基或同一菌株在不同培养基中菌数与对试虫的毒性无关。因此,单纯以活孢子数为指标评价苏云金杆菌不同菌株和不同发酵条件下的杀虫活性是不科学的。

 
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