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发酵条件下
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    THE COMPARISON OF VARIOUS STRAINS OF AUREOBASIDIUM PULLULANS PRODUCING PULIULA AT DIFFERENT CONDITIONS
    几株出芽短梗霉在不同发酵条件下产生多糖的比较
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    Under the optimal fermentation conditions,the activity of fibrinolysin reached 3643 U/ml(urokinase unit) in shake flask,and 2050 U/ml(urokinase unit) in 15 L fermentor.
    在最适发酵条件下,摇瓶发酵产豆豉溶栓酶酶活达到3643U/mL发酵液(尿激酶单位),15L发酵罐发酵产豆豉溶栓酶酶活达到2050U/mL发酵液(尿激酶单位)。
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    When fermentations were conducted in anaerobic bottles,the final succinic acid concentration of SF-9(35.09 g/L) increased 21.4%,and the mass ratio of succinic acid/acetic acid increased from 3.3 to 7.5 compared with those of the parent strain.
    以50 g/L的葡萄糖为碳源,在培养瓶厌氧发酵条件下其琥珀酸产量(35.09 g/L)比出发菌株(28.91 g/L)提高了21.4%,琥珀酸/乙酸比率(w/w)从3.3∶1提高到7.5∶1。
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    In the same measure , the biomass was 10.25 mg/L, iron content of the yeast cell was 7.43 mg/g.
    利用60Co辐射诱变,选育出一株正突变菌株R4-5-27,在同等发酵条件下,生物量达到10.25mg/L,铁含量7.43mg/g,总铁含量为76.16mg/L,比出发菌株提高了1.59倍。
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    The enzyme activity reached 22.37U/ml in fermentation. When the L-asparaginase was treated by chemical precipitation process with ammonium sulfate,30.8% yield of enzyme with a specific activity of approximately 0.55U/mg was obtained and the purity of product was increased 9.85 fold.
    在此发酵条件下,L-天冬酰胺酶发酵液酶活力的峰值可达到22.37 U/ml、产酶稳定期持续7~8 d,利用吴氏提取工艺可以得到回收率为30.8%,纯化倍数为9.85,比活力为0.55 U/mg的L-天冬酰胺酶。
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One Saccharomyces cerevisiae strain, YH4, was selected from fermented solid substrate in Yanghe Distillery, It's thermotolerant and of potential prospect in the application of heavily flavour Chinese liquor production in summer seasons. The microbiological and applied characteristics of the strain were investigated. In addition, the methods of simulated solid state fermentation were developed or modified.The optimal temperature for growth of the strain ranges from 35℃ to 38 ℃ , and the maxium, 47℃ .Although...

One Saccharomyces cerevisiae strain, YH4, was selected from fermented solid substrate in Yanghe Distillery, It's thermotolerant and of potential prospect in the application of heavily flavour Chinese liquor production in summer seasons. The microbiological and applied characteristics of the strain were investigated. In addition, the methods of simulated solid state fermentation were developed or modified.The optimal temperature for growth of the strain ranges from 35℃ to 38 ℃ , and the maxium, 47℃ .Although its optimal pH (pH4-6) is not particular, the strain is tolerant towards the compound organic acids existing in the fermented substrate. The power and rate of fermentation is moderate. At the temperature which is closed t'o that of the average in summer, the application of the strain does remarkably increase the content of ethanol and not decrease that of ethyl hexanoate in distillate from the fermented solid medium, with the inoculum size of not more than 2%.

Saccharomyces cere(?)isiae YH(?)是从洋河酒厂处于顶火期的酒醅中分离出来的耐高温酵母,最适生长温度为35~38℃,最高生长温度为47℃。最适生长PH为4~6,对黄水中复合有机酸有较强的耐性。酒精发酵能力中等,发酵速度较慢。在实验室模拟固态发酵条件下,当对酒醅的接种量在2%以下时,将会使酒精产量明显增加,而且酒中的己酸乙酯含量不受显著影响,因此,该菌株在克服或减轻浓香型白酒夏季生产“掉排”现象中较有应用前途。 本文介绍了YH(?)的微生物学特征及应用特性,同时,探索和改进了一些实验室进行固态模拟发酵的方法。

Abstract Undecanted wine line liquiad which contains 17-24% sugar is made from honey ,then put in 5%(V/v) yeast culture fluid, so that the fermenting speed is higher than traditional way. Output of alcohol is a direet propprtion to the adhereing area in the condition. the method method have been practised in making mead.

用蜂蜜制成的含糖17~24%的醪液,加入5%(V/V)酵母培养液,增加酵母在醪中的附着面积,可使发酵加快,在同等条件下,单位时间内酒精产量比传统法高。酒精产量与增加的附着面积两者之间成正比。在液态发酵条件下,本方法具有实用价值。

he medium in which the polysaccharides in Dictyophora indusiata were produced in the highest amount under the submerged fermentation was selected, and the production curve of polysaccharides was obtained.The fermentation medium is sucrose 2%,corn power 1%, yeast extract 0. 2%,KH_2PO_4 0.3%,MgSO_4 0. 15%,and the juice of bamboo leaves 20%, nature pH. The transformation ratio of inorganic selenium(Se) by D. indusiata was detected, and the results suggested that the ratio was highest at 3ppm of Se.

本文筛选了在深层发酵条件下竹荪多糖产量最高的培养基,得到了竹荪多糖产生曲线,并初步测定了竹荪对无机硒的转化率。用正交试验,确定发酵瓶配方为:麦芽糖2%,玉米粉1%,酵母膏0.2%,KH_2PO_40.3%,MgSO_40.15%,以粽子叶煮汁(20%)配制,自然pH。以DNS法测糖,确定多糖产生曲线。以3ppm时竹荪对硒的转化率最高。

 
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