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胃腺癌细胞系
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  gastric adenocarcinoma cell line
     The Differentiation of Human Gastric Adenocarcinoma Cell Line MGc 80-3 Induced by Retinoic Acid (RA) in Vitro
     维生素A酸(RA)诱导人胃腺癌细胞系MGc 80-3分化
     Cytotoxic activity of TDLN cells to gastric adenocarcinoma cell line KATO3 and human melanoma cell line A375 were compared between 3 groups.
     比较 3组TDLN细胞对胃腺癌细胞系KATO3和黑色素瘤细胞系A375的杀伤作用。
短句来源
     [Methods] SGC7901/VCR(the human gastric cancer multidrug resistance cell subline),SGC7901 and BGC823(the human gastric adenocarcinoma cell line)were cultured in RPMI1640 respectively. The expression of mdr1 mRNA was detected by RT-PCR and hybridization in situ. The P-gp expression was detected by immunoblotting and immunohistochemistry.
     方法培养SGC7901/VCR(人胃癌多药耐药细胞亚系)、SGC7901和BGC823(人胃腺癌细胞系)于RPMI1640,用RT-PCR和原位杂交检测mdr1mRNA的表达,用免疫印迹和免疫组化检测P-gp的表达,用流式细胞仪检测阿霉素在细胞内的蓄积。
短句来源
     Eukaryotic small interfering RNAs (siRNA) expression vector specific for Cyclooxygenase-2 (COX-2) was constructed and transfected into gastric adenocarcinoma cell line AGS.
     构建了可在真核细胞中表达针对COX-2基因的siRNA表达载体,并用胃腺癌细胞系AGS建立了COX-2siRNA的稳定转染细胞系。
短句来源
     Methods The expression of VEGF, Ephrin B2 and Eph B4 in human gastric adenocarcinoma cell line SGC-7901 was observed 48 h after nordy treatment with immunocytochemistry and image analysis.
     方法采用细胞培养、透射电镜、免疫细胞化学和图像分析等方法观测诺帝作用前后人胃腺癌细胞系SGC-7901细胞形态及VEGF、EphrinB2和EphB4蛋白表达的变化。
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  “胃腺癌细胞系”译为未确定词的双语例句
     Methods The gastric cell line SGC-7901 were treated with curcumin in different concentration. The expression of P300,P53,c-myc mRNA were assayed by reverse transcriptase polymerase chain reaction(Rt-PCR).
     方法用不同浓度的姜黄素作用人胃腺癌细胞系SGC-7901,作用24、48 h后,Rt-PCR法检测P300、P53c、-myc mRNA的表达;
短句来源
     pSEAP2? Control Vector was used to detect the efficiency of transfection. After 3 days, the expression of the inserted genes were detected by RT-PCR and ELISA.
     重组质粒glmM/pVAX1、oipA/pVAX1经大量扩增后,瞬时转染胃腺癌细胞系SGC-7901,用pSEAP2—Control Vector作共转染检测转染效率,3天后,用RT—PCR、ELISA检测目的基因表达情况。
短句来源
     ELECTRON MICROSCOPIC OBSERVATION ON THE INDUCED DIFFERENTIATION OF GASTRIC CANCER CELLS(SGC-7901) IN VITRO BY HEXAMETHYLENE BISACETAMIDE
     六亚甲基双乙酰胺对人胃腺癌细胞系(SGC-7901)诱导分化的电镜观察
短句来源
     Four partial overlapping cystatin geng fragments were synthesized, and the full length of sv-cystatin cDNA was obtained and linked to pUC18 vector. Expression plasmid pcDNA3.1/sv-cystatin was constructed and transfected into gastric carcinomar cell line SGC-7901 using lipofectamine gene transfer technique.
     方法:采用人工拼接方法合成蛇毒cystatincDNA,构建pcDNA3.1/sv-cystatin真核表达质粒,经脂质体转染将pcDNA3.1/sv-cystatin质粒和pcDNA3.1质粒分别导入胃腺癌细胞系SGC7901;
短句来源
     Effects of Ursodeoxycholic Acid on the Growth and Morphology of Human Gastric Adenocarcinoma Cells MGc80-3
     熊去氧胆酸对人胃腺癌细胞系MGc80-3生长和形态结构的影响
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  相似匹配句对
     (2)Heterogeneity does exist in gastric adenocarcinoma cell line.
     (2)胃腺癌细胞具有肿瘤异质性;
短句来源
     Production and Differentiation of the Monoclonal Antib(?)dies against Gastric Adenocarcincma cell GC 7911
     抗胃腺癌细胞GC 7911单克隆抗体的研究及鉴定
     Apoptosis induction of gastric adenocarcinoma cell line SGC-7901 by paclitaxel in vitro
     紫杉醇诱导体外培养胃腺癌细胞SGC-7901凋亡
短句来源
     RESPONSE OF A HUMAN GASTRIC ADENOCARCINOMA CELL LINE TO RADIATION
     人体胃腺癌细胞放射敏感性观察
短句来源
     Selection of gastric adenocarcinoma subline and the comparison of biological characteristics between the parent and subline
     胃腺癌细胞侵袭亚的筛选及母、亚两生物学特性的比较
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  gastric adenocarcinoma cell line
The differentiation of human gastric adenocarcinoma cell line MGc80-3 induced by dibutyryl cAMPin vitro
      
Isolation and characterization of an adriamycin-resistant subline of the human gastric adenocarcinoma cell line
      
TGIF activity was determined by growth inhibition of a human gastric adenocarcinoma cell line, MK-1 cells, and IFN-γ activity was measured by radioimmunoassay.
      
Unresponsiveness to interferon associated with STAT1 protein deficiency in a gastric adenocarcinoma cell line
      
Here we report a gastric adenocarcinoma cell line, AGS, that is defective in its response to either IFNα or IFNγ.
      
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To cell culture medium of human stomach glandular carcinoma,cell line SGC-7901, 1mmol/L of cAMP and 1 mmol/L theophylline was added,The growth rates of the cultured cell was obviously depressed,SEM observation showed the regression of microvilli on the cell surface,and at the same time,TEM observation showed a mark reduction of Na~+-K~+ -ATPase activities. Exogenic cAMP and theophylline would elevate the intracellular content of cAMP,and the regression of microvilli and reduction of ATPase activity may indicate...

To cell culture medium of human stomach glandular carcinoma,cell line SGC-7901, 1mmol/L of cAMP and 1 mmol/L theophylline was added,The growth rates of the cultured cell was obviously depressed,SEM observation showed the regression of microvilli on the cell surface,and at the same time,TEM observation showed a mark reduction of Na~+-K~+ -ATPase activities. Exogenic cAMP and theophylline would elevate the intracellular content of cAMP,and the regression of microvilli and reduction of ATPase activity may indicate the reversion of the cancer cell.

本实验通过外源性 cAMP 和茶碱对人胃腺癌细胞系 SGC-7901的连续作用,观察到外源性 cAMP 和茶碱对该细胞株的增殖,具有明显的抑制作用。在透射电镜和扫描电镜下,均可见到细胞表面较光滑,微绒毛减少或消失。同时见到细胞膜表面上的 Na~+—K~+—ATP 酶活性受到抑制。本文讨论了外源性 cAMP 对人胃腺癌 SGC-7901细胞系增殖的抑制作用,以及膜表面微绒毛和Na~+—K~+—ATP 酶活性变化的关系。

Pingyangmycin (PYM), produced by Streptomyces pingyangensis n. sp., was found to be identical to bleomycin A5. In the present study, a comparative observation was carried out ia 10 human cancer cell lines. As determined by a colony-forming assay, the dose-response curves obtained from cells exposed to PYM for 1 h were of one type only: biphasic exponential. The sensitivities of these cells derived from different types of tumors, however, varied with a broad range of ID50 values (0.03-0.82μg/ml).A hepatoma cell...

Pingyangmycin (PYM), produced by Streptomyces pingyangensis n. sp., was found to be identical to bleomycin A5. In the present study, a comparative observation was carried out ia 10 human cancer cell lines. As determined by a colony-forming assay, the dose-response curves obtained from cells exposed to PYM for 1 h were of one type only: biphasic exponential. The sensitivities of these cells derived from different types of tumors, however, varied with a broad range of ID50 values (0.03-0.82μg/ml).A hepatoma cell line (BEL-7402) and three lines derived from squamous carcinomas of the esophagus (Eca109 and CaEs17) or the nasopharynx (CNE) were relatively sensitive (ID50<0.20μg/ml) to PYM which is known to have clinical activity against these diseases. Two gastric adenocarcinoma cell lines (MGc80-3 and BGC-823) and a pulmonary adenocarcinoma cell line (SPC-A-1) appeared to be less sensitive to the drug, with ID50 values of 0.21-0.47μg/ml. PYM was 7-fold more effective against LTEP-78 cells derived from pulmonary squamous carcinoma as opposed to SPC-A-1 cells, resulting in a low ID50 value of 0.04μg/ml. However, PYM as a single agent has not yet received full evaluation in relation to this type of lung cancer. In contrast with other cell lines of squamous cancer origin, HeLa and CC-801 cells derived from uterine cervix cacinomas which have been evaluated as highly responsive to PYM had the highest ID50 values (>0.70μg/ml).

采用集落形成试验法,对平阳霉素(pingyangmycin,PYM)的体外抗癌谱进行研究,结果10种人癌细胞系对PYM的敏感性存在差异,ID_(50)为0.03~0.82μg/ml。食管癌Eca109和CaEs17细胞、鼻咽癌CNE细胞及肝癌BEL-7402细胞对PYM敏感,ID_(50)<0.2μg/ml,与PYM的临床疗效一致;2种起源于宫颈癌的细胞系对PYM明显抗拒,ID_(50)>0.7μg/ml,与临床疗效不一致;2种胃腺癌细胞系(MGc80-3和BGC-823)对PYM的敏感性较低,ID_(50)为0.21和0.47μg/ml;肺鳞癌LTEP-78为PYM敏感细胞,ID_(50)仅为肺腺癌SPC-A-1细胞的1/7,但其临床意义尚不清楚。

The changes of the human gastric adenocarcinoma cell line ( MGc80-3 ) on the charicteristics of growth, morphology and. ultrastructure after the treatment with n-sodium butyrate were studied preliminarily. The results indicated that 1.5 mM n-sodium butyrate could effectively inhibit the generation of MGc80-3 cell line. After treatment with the drug, the observation of light microscopy showed that the cells increased in size, spread fully, while the nuclear-cytoplasmic ratio decreased. The observation of electron...

The changes of the human gastric adenocarcinoma cell line ( MGc80-3 ) on the charicteristics of growth, morphology and. ultrastructure after the treatment with n-sodium butyrate were studied preliminarily. The results indicated that 1.5 mM n-sodium butyrate could effectively inhibit the generation of MGc80-3 cell line. After treatment with the drug, the observation of light microscopy showed that the cells increased in size, spread fully, while the nuclear-cytoplasmic ratio decreased. The observation of electron microscopy showed that the microvilli on the cell surface diminished and was replaced by the ragged and vesicle-shaped structures, The structures of the nuclei and nucleoli were changed obviously. The Go-1gi complex was larger and increased in number, with typical morphology. The rough nedoplasmic reticulum elongoed and the number of free ribosomes decreased. The results mentioned above suggested that these properties on the growth, morphology and ultrastructure could be used as one of the indexes during the study of induced differentiation.

本实验观察到正了酸钠能有效地抑制人胃腺癌细胞系的增殖,查明其生长特性。在光镜下看到,经过正丁酸钠处理的胃癌细胞体积增大,充分铺展,核与质比例下降。电镜下可见这些细胞表面微绒毛减少或消退;高尔基复合体组数增多,体积较大,具有典型的完整结构;粗面内质网变长;游离核糖体减少。这两类形态结构的变化可作为癌细胞分化的指标。

 
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