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   荧光定量 在 心血管系统疾病 分类中 的翻译结果: 查询用时:0.222秒
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荧光定量
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  “荧光定量”译为未确定词的双语例句
    Results The correlation coefficiency was over 0. 99 for standard curves of RT-FQ-PCR method.
    结果建立的实时荧光定量PCR方法的标准曲线相关系数>0.99。
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    Conclusion Quantification of WT1 expression level by RT-FQ-PCR may be used to predict relapse and monitor MRD for acute leukemia patients.
    结论实时荧光定量PCR技术检测急性白血病患者WT1基因表达量可预测难治复发及用于MRD的检测。
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    Real-time quantitative PCR was performed on RNA to detect MMP-3 gene expression in cells.
    应用实时荧光定量聚合酶链反应的方法检测细胞内基质金属蛋白酶3基因的表达量。
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    Expression and significace of mdr_1 in patients with acute leukemia
    荧光定量RT-PCR检测mdr_1在急性白血病中的表达及临床意义
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    Establishment of a real time quantitative-PCR assay for detection of TCR VγI-Jγ gene rearrangement in acute lymphoblastic leukemia patients
    实时荧光定量PCR检测急性淋巴细胞白血病患者TCR VγI-Jγ基因重排
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Thispaperisaimedatresearchingthestructuralbasisandpathogenesisofplateletag-gregabityinpatientswithcoronaryarterydisease(CAD).PlateletboundfibrinogenandmembraneGPⅢa(glcoproteinⅢa)weremeasuredbyflowcytometryin41patientswithCAD.Theresultswereasfolowing:(1)plateletboundfibrinogeninpatientswithunstableanginapactoris(UA)andacutemyocardialinfarction(AMI)werehigherthanthatinstableanginapectoris(SA)groupandnormalsubjects.TherewasnodiferenceofthisindexbetweenSAgroupandnormalsubjects(P>0.05).(2)TherewasnodiferenceofplateletmembraneGPⅢaamongeachgroup(P>0.05).Con-clusion:(1)ThehighfibrinogenbindingtoplatelectsmightbeapathophysiologicbasisofhighplateletaggregabilityinpatientswithCAD.Itwasalsooneofthemajorpathogeneticmechanismofacutemyocardialischemicsyndrome.(2)IncreaseofpleteletboundfibrinogeninpatientswithUAandAMIdidnotresultfromthequantitativechangeofGPⅡb-Ⅲa....

Thispaperisaimedatresearchingthestructuralbasisandpathogenesisofplateletag-gregabityinpatientswithcoronaryarterydisease(CAD).PlateletboundfibrinogenandmembraneGPⅢa(glcoproteinⅢa)weremeasuredbyflowcytometryin41patientswithCAD.Theresultswereasfolowing:(1)plateletboundfibrinogeninpatientswithunstableanginapactoris(UA)andacutemyocardialinfarction(AMI)werehigherthanthatinstableanginapectoris(SA)groupandnormalsubjects.TherewasnodiferenceofthisindexbetweenSAgroupandnormalsubjects(P>0.05).(2)TherewasnodiferenceofplateletmembraneGPⅢaamongeachgroup(P>0.05).Con-clusion:(1)ThehighfibrinogenbindingtoplatelectsmightbeapathophysiologicbasisofhighplateletaggregabilityinpatientswithCAD.Itwasalsooneofthemajorpathogeneticmechanismofacutemyocardialischemicsyndrome.(2)IncreaseofpleteletboundfibrinogeninpatientswithUAandAMIdidnotresultfromthequantitativechangeofGPⅡb-Ⅲa.

为探讨冠心病患者血小板聚集性增强的结构基础和病理机制,我们应用流式细胞术对41例冠心病患者血小板膜结合纤维蛋白原(Fg)和膜糖蛋白(GP)Ⅱb和GPⅢa进行了免疫荧光定量测定。41例冠心病患者中,急性心肌梗塞(AMI)14例,不稳定性心绞痛(UA)12例,稳定性心绞痛(SA)15例。结果表明,血小板膜结合Fg在SA、UA及AMI之间呈递增趋势,差异均有显著性,且AMI和UA患者该指标均高于正常组(P<0.05),SA患者与正常组间差异无显著性(P>0.05);各观察组间血小板膜GPⅡb和GPⅢa量差异均无显著性,且与血小板膜结合Fg量之间无直线相关性。提示:血小板膜结合Fg量的增加是AMI和UA患者血小板聚集性增高的病理基础,是血小板在急性不稳定性心肌缺血机制中的早期主要变化之一,具有加重冠状动脉病变的作用;血小板膜结合Fg的增加并非膜GPⅡb-GPⅢa数量改变所致。

Objective: To determine the incidence of human cytomegalovirus(HCMV) infection and the quantity of the virus in the peripheral blood in preoperative and postoperative renal allograft recipients. Method: 61 preoperative and 32 postoperative renal allograft recipients were detected by quantitative AmpliSensor PCR. The incidence of HCMV infection and the quantity of HCMV in peripheral blood were subsequently analysed by χ 2 test and t test,respectively. Results: The incidence of HCMV infection in preoperative...

Objective: To determine the incidence of human cytomegalovirus(HCMV) infection and the quantity of the virus in the peripheral blood in preoperative and postoperative renal allograft recipients. Method: 61 preoperative and 32 postoperative renal allograft recipients were detected by quantitative AmpliSensor PCR. The incidence of HCMV infection and the quantity of HCMV in peripheral blood were subsequently analysed by χ 2 test and t test,respectively. Results: The incidence of HCMV infection in preoperative and postoperative renal allograft recipients were 77% and 87 5%,respectively. Their quantities of HCMV in peripheral blood were 1.07×10 7 L -1 and 7.41×10 7 L -1 , respectively. The difference in incidence of HCMV infection was not significant ( χ 2 =1.65, P >0 05), and the difference in HCMV quantity was highly significant ( t =6.46, P <0 01). Conclusions: Results indicate that ①immunosuppressive therapy reactivates latent HCMV infection; ②status of HCMV infection is chiefly determined by recipient′s own immunity. In order to decrease the incidence of HCMV infection, it is more important to make use of immunosuppressant reasonably and avoid overimmunosuppression.

目的:研究肾移植受者手术前后人巨细胞病毒(HCMV)的感染率及HCMVDNA含量变化。方法:采用半套式荧光定量聚合酶链反应(AmpliSensorPCR)方法定量检测术前肾移植受者61例,术后32例,用四格表比较手术前、后的感染率,用t检验比较病毒含量。结果:手术前、后肾移植受者HCMV的感染率分别为77%和875%;HCMVDNA平均含量(拷贝数每升)分别是107×107L-1和741×107L-1,两者之间感染率无显著差异(χ2=165P>005),但病毒平均含量存在极显著差异(t=646P<001)。结论:研究结果显示:①免疫抑制治疗激活了潜伏性HCMV感染,导致术后HCMV含量显著增加;②决定肾移植受者HCMV感染状态的主要是机体本身的免疫力,减少HCMV感染,术后合理使用免疫抑制剂,避免过度抑制可能更重要

The expression of multi-drug resistance gene(mdr 1 mRNA)was measured in 36 patients with different type of acute leukemia and in 15 normal controls by fluorogenic probe quantitative RT-PCR(FQ-RT- PCR). Result showed that the mdr 1 mRNA was neg ative in normal controls. The average level of the mdr 1 gene express in untreated group was 2.7×10 3 copies/μgRNA,while that of clinical resistance group was 1.0×10 4 copies/μgRNA(P<0.05).The CR rate...

The expression of multi-drug resistance gene(mdr 1 mRNA)was measured in 36 patients with different type of acute leukemia and in 15 normal controls by fluorogenic probe quantitative RT-PCR(FQ-RT- PCR). Result showed that the mdr 1 mRNA was neg ative in normal controls. The average level of the mdr 1 gene express in untreated group was 2.7×10 3 copies/μgRNA,while that of clinical resistance group was 1.0×10 4 copies/μgRNA(P<0.05).The CR rate differed significantly between mdr 1 positive group(21%)and negative group(82%)(P<0.05).It suggests that FQ-RT-PCR is an acurate quantitative method in detecting the mdr 1 gene expression,a high level of mdr 1 expression correlated closely with drug resistance in clinical leukemia chemotherapy.

采用荧光定量 RT- PCR法检测 36例不同类型白血病患者的多药耐药基因 (mdr1 m RNA)表达 ,同时检测 15例骨髓或外周血正常患者 ,以做对照。结果对照组均为阴性表达 ,初治患者组 m dr1 阳性基因拷贝数平均为 2 .7× 10 3拷贝 / μg RNA;复发难治患者组为 1.0× 10 4拷贝 / μg RNA(P<0 .0 5 )。表达阴性 m dr1 与 mdr1 阳性患者的完全缓解 (CR)率分别为 82 %和 2 1% (P<0 .0 5 )。提示荧光定量 RT- PCR检测 mdr1 基因表达结果用绝对拷贝数表示 ,其定量准确、可靠。临床急性白血病化疗耐药的发生主要与 mdr1 阳性高表达有关。

 
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