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翻译区
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  untranslated region
    A Regulation-related Intron in 5' Untranslated Region of Rice Waxy Gene
    水稻蜡质基因5'非翻译区一个与调控有关的内含子
短句来源
    The acquired gene was 789 bp in full length, including 5' untranslated region of 90 bp, 3' untranslated region of 276 bp with Poly A, and an open reading frame (ORF) encoding 140 amino acid.
    基因全长789bp,5'非翻译区90bp,3'非翻译区276bp,开放阅读框编码140个氨基酸。
短句来源
    The full-length cNDA of G lucidum MT was of 315bp by including a 5′untranslated region(UTR) of 47bp, a 3′UTR of 166bp and an open reading frame (ORF) of 102bp encoding a polypeptide of 33 amino ancids.
    该序列全长为315bp,5′-端非翻译区为47bp,3′-端非翻译区具有166bp,开放阅读框长度为102bp(包括一个终止密码子),可编码33个氨基酸。
短句来源
    3′Untranslated Region of Eukaryotic mRNA in Gene Regulation
    真核mRNA3′非翻译区在基因表达中的作用
短句来源
    Affects of the 3′ untranslated region on expression of recombinant human hepatopoietin
    3′端非翻译区对重组人肝细胞生成素表达不均一性的影响
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  terminal region
    Sequence analysis revealed a 1028bp cDNA which contains the 67bp 5' terminal region, 597bp open reading frame (ORF) and 364bp 3' terminal region. The whole ORF encoded a NKEF protein of 198 amino acids.
    牙鲆NKEFcDNA全长1028bp(不包含polyA),其中包括67bp的5’非翻译区、597bp的开放阅读框和364bp的3’非翻译区(不包含polyA),整个开放阅读框编码198个氨基酸。
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  “翻译区”译为未确定词的双语例句
    Modification and complete sequence analysis of human transforming growth factor-β_1 cDNA
    人转化生长因子β_1基因非翻译区的改造及其全长序列测定
短句来源
    Recent Advances on the Functions of the 3'-Untranslated Regions of Eukaryotic mRNA' s.
    真核mRNA3′非翻译区的功能研究进展
短句来源
    Effect of 5'non-coding Region on Expression of LT-B Gene
    5′端非翻译区对LT-B基因表达的影响
短句来源
    Influence of 5'-Untranslated Region (5'-UTR) on the Regulation of Human Tissue-type Plasminogen Activator (t-PA) mRNA Expression
    人组织型纤溶酶原激活剂(t-PA)mRNA5′端非翻译区(UTR)对其表达的调控
短句来源
    Effect of 3'-untranslated Region on Expression of TNFa cDNA in Escherichia coli
    3′端非翻译区影响TNFα cDNA在大肠杆菌中的表达
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  untranslated region
Stability of plant mRNAs depends on the length of the 3'-untranslated region
      
The key role in this is played by ciselements of the 5"-untranslated region (5"-UTR) and, in particular, by the internal ribosome entry site (IRES).
      
Brain-Specific Sequence Hfb1 Is a Part of the Large 3"-Untranslated Region of the Human Complexin 2 Gene: New in vitroand in sil
      
Hfb1 proved to correspond to a new gene exon which codes for a large 3"-untranslated region of the mRNA for synaptic protein complexin 2.
      
A GenBank search revealed complete identity of the 5" end of Ghfb and the 3"-untranslated region (878-933) of the human complexin 2 mRNA.
      
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Identification of HLA-DR alleles at DNA level is a complementary typing technique applicable to any nucleated cells.Although it is yet unknown that whether the DNA polymorphism detected in homozygous typing cells with unambiguous DR types defined by serotyping is of functional significance,it is reasonable at present status to include DNA polymorphism in the DR typing.One of the major problems in DNA typing is to have the complex patterns of the hybridized DNA fragments logically interpreted.It is particularly...

Identification of HLA-DR alleles at DNA level is a complementary typing technique applicable to any nucleated cells.Although it is yet unknown that whether the DNA polymorphism detected in homozygous typing cells with unambiguous DR types defined by serotyping is of functional significance,it is reasonable at present status to include DNA polymorphism in the DR typing.One of the major problems in DNA typing is to have the complex patterns of the hybridized DNA fragments logically interpreted.It is particularly involved in the heterozygous individuals.Hoping to reduce the number of hybridized DNA fragments and hence the complexity of RFLP,we constructed subprobes from the full-length DRβ DOβ and DQα cDNA probes.It was found that genomic DNA's isolated from unambiguously se-lotyped HTCs,digested with PvuII,and hybridized to a DRfJ 3' subprobe containing 3' untranslated region sequence,showed RFLP patterns distinct among DR alleles.Although further characterization is needed for DNA typing to be a practical feasible technique,the simplified patterns of the hybridized DNA fragments facilitate the DNA typing in individuals heterozygous for some haplotypes.It was found that the number of hybridized DNA fragments corresponds to the number of DRβ genes in DRl-DRw8 haplotypes.Moreover,the results obtained showed that DR3,DR5,and DRw6 have a common hybridization pattern,suggesting that they might have evolved from a common ancestral gene.The same situation was seen in DR4,DR7,and DRw9,which might have evolved in an similar way.

在DNA水平上鉴定HLA-DR等位基因是一种适用于任何有核细胞的分型技术。DNA分型的主要问题是解释Southern印迹分析中杂交片段的复杂格局,这在杂合个体尤为困难。为此,我们从DRβ、DQβ和DQα全长cDNA探针建立了亚探针,以便减少杂交片段的数目,从而降低限制片段长度多态性(RFLP)的复杂性。我们发现内切酶PvuⅡ和DRβ3'端不翻译区亚探针,而对一些DR等位基因作出鉴定。这些简化了的杂交片段格局有利于对一些杂合个体作DNA分型。

cDNA clones encoding Chinook Salmon, Oncorhynchus tschawytscha, growth hormone (sGH) have been islated from a cDNA library prepared from Chinook Salmon pituitary gland pnly(A)+ RNA. Synthetic oligodeoxnucleotide mixtures based on amino acid residues 1-7 of sGH and 166-172 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids including...

cDNA clones encoding Chinook Salmon, Oncorhynchus tschawytscha, growth hormone (sGH) have been islated from a cDNA library prepared from Chinook Salmon pituitary gland pnly(A)+ RNA. Synthetic oligodeoxnucleotide mixtures based on amino acid residues 1-7 of sGH and 166-172 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids including a putative signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the message were 70 and 446 bases long, respectively. Comparison of the nucleotide sequence and amino acid sequence between Chum sGH and Chinook sGH indicated that there were 97% and 99% homology respectively.

从太平洋切奴克鲑鱼(Pacific Chinook Salmon,Oncorthychus tschawytscha)垂体poly(A)~+ RNA构建cDNA文库。按照鲑鱼生长激素(sGH)部分氨基酸序列合成两个寡聚脱氧核苷酸探针,它们分别与编码第1—7和第166—172氨基酸序列互补。用探针筛查cDNA文库,得到了完整的sGH cDNA克隆。cDNA序列已测定,包括编码210个氨基酸的编码序列。其中含有22个氨基酸的信号肽序列和188个氨基酸的成熟GH序列。该克隆还包括了5'端和3'端非翻译区,分别为72个和438个碱基对长。与Chum鲑鱼比较表明,核酸序列和氨基酸序列的同源性分别为97%和99%。

There is a 15bp large reverse repeated sequence proceeded by a 7bp small one in the 3'flan-king region of rbcL of Nicotiana tabacum. A 383 bp of Xbal fragment containing these tandem-ly repeats was inserted into the plasmid pXSA, ai the position between the λp and the cat gene. Then these two repeats were separated and deleted systematically to obtain various deletions. The deletion pRT65, pRT74 and.pRT83 was sequenced to determine the deleted base pairs exactly.S1 mapping analysis was adopted to investigate...

There is a 15bp large reverse repeated sequence proceeded by a 7bp small one in the 3'flan-king region of rbcL of Nicotiana tabacum. A 383 bp of Xbal fragment containing these tandem-ly repeats was inserted into the plasmid pXSA, ai the position between the λp and the cat gene. Then these two repeats were separated and deleted systematically to obtain various deletions. The deletion pRT65, pRT74 and.pRT83 was sequenced to determine the deleted base pairs exactly.S1 mapping analysis was adopted to investigate the transcripts of these deletions in E. coli JM83. The results showed us that the stability of mature 306bp mRNA relied on the large repeat and a short sequence downstream. The small one was not efficient.The regulation level of the rbcL termination was also investigated. The Xbal-EcoRI fragments from pRT65, pRT74 and pRT83 were transferred into pSP-TT at the position between the spinach promoter and threonine terminator to construct pRT65, pRT74 and pRT83 respectively. The results from SI mapping analysis showed that the E. coli RNA polymerase read through the 3' flanking region of rboL gene and terminated at the stop site of threonine terminator. These results suggested that mature rbcL mRNA might be the product precisely processed from a precursor mRNA and the 3' flanking sequence might be the signal for precursor mRNA to be processed to the correct position.

对烟草(Nicotiana tobacum)rbcL转录单位3'端前后相连的2个逆向重复序列作系统删减并单独将此2个序列分开。在原校体系中考察这些缺失子的转录物,结果表明,成熟rbcL mRNA的积累依赖于3'端的大逆向重复序列及未翻译区的一段序列。 将带有逆向重复序列的片段插入菠菜rbcL启动子及苏氨酸终止子之间,大肠杆菌的RNA多聚酶读过rbcL3'端的逆向重复序列而全部停止在苏氨酸终止子的终止位点,表明成熟的rbcL mRNA不是rbcL转录的直接产物,而是由前体mRNA经转录后的加工过程后形成的。

 
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