AIM: To investigate the polymorphism of tumor necrosis factor-α (TNF-α) gene promoters in Chinese Han people, and to clarify whether such polymorphism was associated with the outcome of hepatitis B virus infection.
目的:探讨中国汉族人肿瘤坏死因子-α(tumor necrosis fac- tor-α,TNF-α)基因启动子单核苷酸多态性与乙型肝炎病毒(hepatitis B virus,HBV)感染结果之间的关系.
DNA Binding Activity of C/EBPβ and C/EBPδ for the Rat α2-Macroglobulin Gene Promoter Is Regulated in an Acute-Phase Dependent Ma
By means of an in vitro phosphorylation assay, South-Western blot, and selective proteolysis we have also found that only abilities of 35-kD C/EBPβ and 27-kD C/EBPδ to bind to the α2- MG gene promoter were affected by phosphorylation.
GATA-3 was shown to bind to two sites of the IL-4 gene promoter in human T-cell lines PER-117 and Jurkat.
Human heart LIM (hhLIM), a muscle-specific expressed transcriptional coactivator of cardiac hypertrophy related gene, stimulates transcriptional activation of the ANF gene promoter in H9C2 cells.
Cloned upstream of the αD gene promoter, this fragment suppressed the promoter activity when all its CpG were methylated.
Construction of a bidirectional promoter and its transient expression in Populus tomentosa
It is important to construct a bidirectional promoter for transforming two or more genes into plants simultaneously.
The widely used unidirectional CaMV35S promoter has been modified to a bidirectional promoter in this work by fusing a CaMV35S minimal promoter (Pmini) at its end in opposite orientation to the original promoter.
The direction of transient expression of GUS and GFP in Agrobacterium mediated 3-4 days transformed leaf discs of Populus tomentosa, indicating that the promoter did have bidirectional transcriptional activities simultaneously in cells and tissues.
It was discussed that this bidirectional promoter could possibly be applied in woody plant engineering.
Regulation of the human Giα-2 gene promotor activity in embryonic chicken cardiomyocytes
Plasmid pFN3 harboured the xynA ORF fused to the yeast mating pheromone alpha-factor signal sequence (MFα1S) under the control of the alcohol dehydrogenase II gene promotor (ADH2P) and terminator (ADH2T) sequences.
In plasmid pFN4, the MFα1S-xynA ORF was brought under the control of the phosphoglycerate kinase I gene promotor (PGK1P) and terminator (PGK1T) sequences.
Constructs containing deletions extending into the glaA gene promotor were introduced into an A.
For that, tobacco was transformed via Agrobacterium tumefaciens with a binary vector containing a tobacco GS-2 cDNA downstream of the leaf-specific soybean ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene promotor.