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  secondary metabolites
Belyakov, Marine Microorganisms and Their Biologically Active Secondary Metabolites,
      
Analysis of Secondary Metabolites of Microscopic Fungi of the Genus Penicillium by Chromatographic Techniques
      
Occurrence of Indole Alkaloids among Secondary Metabolites of Soil Aspergillus Spp.
      
Secondary metabolites of three strains of Penicillium aurantiogriseumisolated from permafrost sediments were identified.
      
It was demonstrated that the fungus Penicillium expansum 2-7, a resident strain of the orbital complex Mir, which became dominant at the end of a long-term space flight, formed biologically active secondary metabolites (antibiotics).
      
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Several varieties of tomato with different degrees of resistance to cladosporium fulvum were used to study the changes in phenylalanine ammonia lyase(PAL) activity and lignin conten as well as their correlationship with the resistance degree of varieties and pathogeny during inoculation.PAL activity increased through inoculation.PAL peaks appeared earlier in resistant varieties than those in susceptible ones.There was significant varieties correlation between the seedling resistance and the increase of PAL activity...

Several varieties of tomato with different degrees of resistance to cladosporium fulvum were used to study the changes in phenylalanine ammonia lyase(PAL) activity and lignin conten as well as their correlationship with the resistance degree of varieties and pathogeny during inoculation.PAL activity increased through inoculation.PAL peaks appeared earlier in resistant varieties than those in susceptible ones.There was significant varieties correlation between the seedling resistance and the increase of PAL activity during 36 hours after inoculation(r=0.96**).The changing speed of PAL activity as a resistance index was applied successfully in identifying the differentiation in physiological varieties of Cladosporium fulvum.

以含对叶霉病不同抗性基因的番茄品种为材料,研究了叶霉病菌侵染过程中PAL酶活性和次生代谢物木质素含量的变化与品种抗性、病原菌生理小种间的相互关系。接种后苯丙氨酸解氨酶活性增加,抗病品种的PAL酶活性高峰出现早于感病品种。番茄品种的抗病性与其接种后36hPAL酶活性变化百分比呈极显著正相关(r=0 96 )。另外,还成功地利用PAL酶活性变化速度这一指标对叶霉病生理小种的变异进行了生化鉴定。

The induction of calli on different explants of Ginkgo biloba and the determination of the flavonol glycosides from its callus by high-performance liquid chromatography (HPLC) were studied. The rate of induction calli from endosperm Ⅰ was highest (100%), the biological yield of calli was up to 893 mg in each flask. The optimum concentration basic medium MS supplemented with 2 mg·L~(-1) 6-BA and  1 mg·L~(-1) NAA promoted the formation of secondary metabolites, which resulted in 8.7 mg·g~(-1) of flavonol glycosides....

The induction of calli on different explants of Ginkgo biloba and the determination of the flavonol glycosides from its callus by high-performance liquid chromatography (HPLC) were studied. The rate of induction calli from endosperm Ⅰ was highest (100%), the biological yield of calli was up to 893 mg in each flask. The optimum concentration basic medium MS supplemented with 2 mg·L~(-1) 6-BA and  1 mg·L~(-1) NAA promoted the formation of secondary metabolites, which resulted in 8.7 mg·g~(-1) of flavonol glycosides.

对银杏不同外植体愈伤组织生长进行研究,并应用高效液相色谱(HPLC)法测定黄酮苷含量.结果表明:银杏胚乳Ⅰ愈伤组织诱导率高达100%,其生物量最高,达893mg·瓶-1;以MS为基本培养基,添加2mg·L-16 BA、1mg·L-1NAA,有利于次生代谢物的合成和积累,黄酮苷含量高达8.7mg·g-1.

Cucumber seedlings were infected with Fusarium oxysporum f.sp.cucumerinum (the density of spores was 10~4 cfu/mL),after 4 weeks growth.The effects of Glmous versiforme on the flavone and total phenol concentration in the roots and POD and PPO activity in the leaves were investigated.The results indicated that resistance of mycorrhizal plants to wilt can be improved by compensating biomass and chan- ging in secondary metabolites.On the 9th day,compared with control,biomass of roots and shoots in- creased by 50%...

Cucumber seedlings were infected with Fusarium oxysporum f.sp.cucumerinum (the density of spores was 10~4 cfu/mL),after 4 weeks growth.The effects of Glmous versiforme on the flavone and total phenol concentration in the roots and POD and PPO activity in the leaves were investigated.The results indicated that resistance of mycorrhizal plants to wilt can be improved by compensating biomass and chan- ging in secondary metabolites.On the 9th day,compared with control,biomass of roots and shoots in- creased by 50% and 31.7% respectively in mycorrhizal plants inoculated with G.v.,for control the dis- ease incidence had reached 15%;mycorrhizal plants were healthy during all the sampling times.On the early and later stages of pathogen invasion,the concentration of flavone and total phenol in roots of my- corrhizal plants were higher than those of control,when the control began to show visible disease symp- toms,the mycorrhizal plants can protect from being further attacked with lower flavone and total phenol concentrations.Compared with control the changes of POD activity were stable in leaves of mycorrhizal plants and PPO activity was higher than that of control.

采用灌根法对4周龄的黄瓜幼苗接种尖孢镰刀菌 Fusarium oxysporum f.sp.cucumerinum,研究了不同取样时期 AM 真菌 Glomus versiforme 对植株根系黄酮和总酚含量及叶片中 POD 和 PPO 酶活性的影响。结果表明:菌根化植株可通过生物量的补偿和体内次生代谢物含量的改变来提高抗病性。即接种 F.oxysporum f.sp.cucumerinum 的第9天,菌根化植株根系和地上部生物量分别比对照高出50%和31.7%,此时,对照发病率达15%,而菌根化植株生长始终正常;在病原菌的侵入初期和后期,菌根化植株根系中黄酮和总酚含量高于对照;在植株发病期,菌根化植株仍可通过根系中含量相对较低的黄酮和总酚来抵御病原菌的侵害;与对照相比,抗病性较强的菌根化植株叶片中 POD 酶活性变化较为缓和,且 PPO 酶活性一直较高。

 
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