Plasma triglyceride(TG),high density lipoprotein-cholesterol(HDL-C),cholesterol(CHO),testosterone(T),estradiol(E_2),prolactin(PRL),luteinizing hormone(LH),and follicle-stimulating hormone(FSH) were determined for comparing their dynamic changes.
IVF/ICSI were performed as routine. Results Serum follicle stimulating hormone and luteinizing hormone (LH) levels on the day of starting Gn were lower in the long protocol group comparing with the short protocol group [(4 4±1 2) IU/L Vs (6 3±1 7) IU/L, (2 7±1 5) IU/L Vs (4 4±2 8) IU/L, P <0 01 respectively].
It is found that these McAbs reacted specially with hCG β but not with human luteinizing hormone(hLH) and follicle stimulating hormone (hFSH). Both of them belonged to mouse IgG1,and the ascitic titres were 3.2×10 -5 (1D 5)and 6.4×10 -5 (4D 5)respectively.
By using the technique of cell hybridization, monoclonal antibodies(MAbs) specific to porcine follicle--stimulating hormone (pFSH) was produced. Among the MAbs, AF05 (affinity constant K= (1. 14±0. 28)×10~(-9)M)was used to make an immuno--affinity chromatography column.
The dynamics of follicle-stimulating hormone (FSH) luteinizing hormone (LH), prolactin (PRL), estradiol (E2), and progesterone were studied in left- and right-handed women having a stable 28-day menstrual cycle.
The data showed that bllod serum levels of FSH, LH, PRL, and E2are higher in left-handed in comparison to right-handed women (p>amp;lt; 0.001).
The experimental data for the case of resonance excitation attest to an interference character of the interaction of optical nonlinearities of different orders in the process leading to the formation of the FSH signal.
An insertion fragment in porcine FSHβ subunit gene was cloned by PCR.
With the combination of genotype and litter size of sows, it was demonstrated that FSHβ locus is closely associated with major gene controlling litter size in commercial pig breeds, such as Yorkshire, Landrace, Durco.