It includes the complete first exon(1~61?bp),first intron(62~703?bp) and partial sequence of exon 2(704~732?bp) in the 732 base pairs of FSHβ gene sequence,and the content of A+T(66.12%) is higher than G+C(33.88%).
Results The luciferase reporter gene construct containing the first intron of α 1(I) collagen gene (-804~+1 452, was called as PGL3 col) had a higher level of gene expression, as compared with the construct lacking the first intron 〔was called as PGL3 col (△intron)〕 in activated HSCs (315±45 U/mg protein vs 220±70 U/mg protein, P <0.05).
Results The luciferase reporter gene construct containing the first intron of α 1(I) collagen gene (-804~+1 452, was called as PGL3 col) had a higher level of gene expression, as compared with the construct lacking the first intron 〔was called as PGL3 col (△intron)〕 in activated HSCs (315±45 U/mg protein vs 220±70 U/mg protein, P <0.05).
In order to study intron effect, whey acid protein(WAP) gene the first intron was cloned to human G-CSF gene 5′end, and this fragment was directed by WAP 2.6kb promoter.
One SNP(-157G/A) was found in intron 1, One SNP(+115C/G) was found in Eexon 2, One SNP(+877G/C) was found in intron 3. 2 The SNP3 was conformed to expectation of the Hardy-Wei nberg rule.
Bioinformatic analysis indicated that putative promoter sequence of the cloned Xinong Saanen Dairy Goat β-casein 5' flanking sequence shared 96%~98% homology with other goat β-casein gene promoters. Xinong Saanen Dairy Goat β-casein 5' flanking sequence region contained promoter sequences and putative recognition sites for several transcriptional factors, including HOXF, Oct1, AP1, CEBP, STAT, NFKB, GR, ER, PR, ETS and the intron 1, exon 1 and exon 2 of the goat β-casein gene.
Artiga et al found there were two hot spot between the 5’ noncoding intron and first exon, ie 106-127bp and 423-443bp. The latter mutation was related with the increase expression of BCL-6. It indicated this region contains a regulatory element.
In 2001, Wilson and his colleagues cloned another two members - human WNK1 gene and WNK4 gene which were located on chromosome 12 and 17, respectively.
As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA.
Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene.
Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA.
As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA.
Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene.
Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA.
Male-specific DNA-protein complexes were revealed with labeled oligonucleotides corresponding to fragments +1150/+1449, +1358/+1449, +1397/+1449, and +1417/+1449 of intron 1 of the Ste1 gene.
RET exons 8, 10, 11, 13, 14, 15, and 16 and intron 1 along with the GFRA1 5'-UTR were screened by PCR and subsequent direct sequencing or RFLP analysis.