第一内含子 first intron(6) the first intron(2) intron 1(3) 目前没有对本词条修改信息

first intron     Construction of Expression Vector of pGH cDNA with First Intron and its Expression in COS7 Cells     含第一内含子猪生长激素cDNA基因在COS7细胞中的表达 短句来源     It includes the complete first exon(1～61?bp),first intron(62～703?bp) and partial sequence of exon 2(704～732?bp) in the 732 base pairs of FSHβ gene sequence,and the content of A+T(66.12%) is higher than G+C(33.88%).     在测定出的FSHβ亚基基因732 bp序列中,包括第一外显子全序列(1~61 bp),第一内含子全序列(62~703 bp)以及第二外显子部分序列(704~732 bp),并且序列中A+T含量(66.12%)高于G+C(33.88%)。 短句来源     The lengthes of the first intron and the second intron were 1 833 bp and 2 018 bp respectively. The full length of Mongolian horse MSTN gene was 4 979 bp and firstly submitted to Genbank.     第一内含子1 833 bp、第二内含子2 018 bp,共有4 979个碱基。 短句来源     Sanhe horse, Wuzhumuqin horseand Xinihe horse lay in a same evolution branch, it was different from other branches of horse. 7、The polymorphisms of PCR-SSCP existed only in the fifth exon, but not in the 5'flanking region, first intron, and second exon.     7、PCR-SSCP 分析结果表明,在所检测的 5'端侧翼区、第一内含子、第二外显子和第五外显子四个位点中,仅第五外显子检测到多态,该多态位点由 A 和 B 两种等位基因控制。 短句来源     The porcine growth hormone cDNA with first intron was constructed in our lab. In order to learn the effect of the first intron on gene expression,the expression vector of pGHcDNA with first intron(pGH cDNAin)under the control of CMV prompter was constructed. By transient expression mediated with DEAEDextran in COS7 cells and detection with PP95 biological method,the pGH cDNAin could be secretary expressed in eucaryotic cells,and expressed pGH showed biological activity.     以CMV真核生物启动子构建了含第一内含子猪生长激素(pGH)cDNA基因(pGHcDNA in)的表达载体,经DEAE 葡聚糖介导在COS7细胞进行了瞬时表达,通过PP95生物学测定法测定,证明含第一内含子pGHcDNA基因能够在真核细胞中进行分泌性表达,表达出的pGH具有生物学活性。 短句来源 更多          the first intron     The lengthes of the first intron and the second intron were 1 833 bp and 2 018 bp respectively. The full length of Mongolian horse MSTN gene was 4 979 bp and firstly submitted to Genbank.     第一内含子1 833 bp、第二内含子2 018 bp,共有4 979个碱基。 短句来源     The porcine growth hormone cDNA with first intron was constructed in our lab. In order to learn the effect of the first intron on gene expression,the expression vector of pGHcDNA with first intron(pGH cDNAin)under the control of CMV prompter was constructed. By transient expression mediated with DEAEDextran in COS7 cells and detection with PP95 biological method,the pGH cDNAin could be secretary expressed in eucaryotic cells,and expressed pGH showed biological activity.     以CMV真核生物启动子构建了含第一内含子猪生长激素(pGH)cDNA基因(pGHcDNA in)的表达载体,经DEAE 葡聚糖介导在COS7细胞进行了瞬时表达,通过PP95生物学测定法测定,证明含第一内含子pGHcDNA基因能够在真核细胞中进行分泌性表达,表达出的pGH具有生物学活性。 短句来源   intron 1     Bioinformatic analysis indicated that putative promoter sequence of the cloned Xinong Saanen Dairy Goat β-casein 5' flanking sequence shared 96%~98% homology with other goat β-casein gene promoters. Xinong Saanen Dairy Goat β-casein 5' flanking sequence region contained promoter sequences and putative recognition sites for several transcriptional factors, including HOXF, Oct1, AP1, CEBP, STAT, NFKB, GR, ER, PR, ETS and the intron 1, exon 1 and exon 2 of the goat β-casein gene.     生物信息学分析显示:该序列与已公布的其它山羊β-酪蛋白基因序列具有极高同源性(96%~98%),包含有TATA启动子序列和HOXF、Oct-1、AP1、CEBP、STAT、NFKB、GR、ER、PR、ETS 等转录因子识别位点,该序列保留了β-酪蛋白第一内含子、第一外显子和第二外显子及其信号肽部分。 短句来源     The results of PCR-SSCP analysis indicate that there is polymorphism only in 5′flanking region site among 4 sites which are 5′flanking region, intron 1, exon 2 and exon 5. As the result, there are mutations in two base sites of horse GH gene sequence: T replaced C at 1719 site, G replaced A at 1743 site.     3、PCR-SSCP 分析结果表明,在所检测的 5′端侧翼区、第一内含子、第二外显子和第五外显子四个位点中,仅第五外显子检测到多态,该多态位点由 A 和 B 两种等位基因控制。 GH 基因第 1719 处一个 T→C 的突变和 1743 处 G→A 的突变,导致等位基因 B变为等位基因 A。 短句来源     4. A new mutation site of intron 1, T→ C translation, was detected. The mutation maybe affects the GH expression and decreases the later daily weight gain.     4.发现了第一内含子上一个新的T→C的替换位点,该位点的替换和GH的表达量存在相关,并对后期的体重增长产生影响; 短句来源   “第一内含子”译为未确定词的双语例句     4. Specific primers P4, P5 and P6 were designed to amplify fragments from chicken A-FABP gene.     4、根据鸡A－FABP基因第一外显子及第一内含子序列设计特异性扩增引物p4－P．，应用PCR－SSCP分析在A－FABP基因上发现P。 、P。 短句来源     The results showed:The all sequence of Mongolian horse MSTN gene was cloned and sequenced and submitted in GenBank (accession AY840554). The result showed: the all sequence was 4979bp including 3 exons and 2 introns, the size of the exons were: 373bp,374bp,381bp; the size of the introns were:1833bp and 2018bp.     首先克隆了蒙古马MSTN基因并进行了全序列测定,测定结果登录在Genbank上,登录号为AY840554,全序列分析结果表明,蒙古马MSTN基因全序列为4979bp,由三个外显子和两个内含子组成,即第一外显子373bp、第二外显子374bp、第三个外显子381bp、第一内含子1833bp、第二内含子2018bp。 短句来源     In order to construct oviduct-specific expression vectors, we cut thesequence of 5'-flanking region of chiken ovalbumin promoter, whichcontains the 5'-f1anking sequence and the first extron and the sequenceencoding first l40 amino acids.     为了制备转基因鸡输卵管特异性表达载体，用PstI直接从基因组上切下鸡卵清蛋白基因5’端4.65kb的启动子序列(简称OVp)，其中包括基因5’侧翼序列、第一外显子、第一内含子以及编码前140个氨基酸的DNA序列。 短句来源     And then the present study selected MSTN as a candidate gene to identify any difference in 2512 bp fragment included one intron and three extrons of MSTN among Wushen, Wuzhumuqin, Xinihe, Sanhe, Thorougnbred and Short Horse by PCR-SSCP, PCR-RFLP.     建立了一种片段在 300bp 左右的 PCR-SSCP 分析方法。 以 MSTN 为候选基因,以乌审马、乌珠穆沁马、锡尼河马、三河马、纯血马、矮马等 6 个品种马为实验材料通过 PCR-SSCP 和 PCR-RFLP 分析技术对其 3 个外显子和包括完整的第一内含子在内的 2512bp 片段进行对比分析。 短句来源     The intronⅠlength was 766bps and the intronⅡlength was 587bps.     第一内含子为766bp,第二内含子为587bp。 短句来源 查询“第一内含子”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句

为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   first intron Ribosomal Protein Binding with the First Intron of the Human rpS26 Pre-mRNA Stimulates Its Interaction with Proteins Extracted f 例句来源       As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA. 例句来源       Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene. 例句来源       The binding was specific, since human rpS19 had an order of magnitude lower Ka with the first intron and did not bind with the rpS26 mRNA. 例句来源       Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA. 例句来源       更多             the first intron Ribosomal Protein Binding with the First Intron of the Human rpS26 Pre-mRNA Stimulates Its Interaction with Proteins Extracted f 例句来源       As shown by nitrocellulose filtration assays with RNA fragments transcribed from various regions of the human ribosomal protein (rp) S26 gene, proteins of the 40S ribosome subunit bind to the first intron of the rpS26 pre-mRNA. 例句来源       Small-subunit proteins did not affect the efficiency of in vitro splicing of a pre-mRNA fragment corresponding to the first intron, second exon, second intron, and a part of the third exon of the rpS26 gene. 例句来源       The binding was specific, since human rpS19 had an order of magnitude lower Ka with the first intron and did not bind with the rpS26 mRNA. 例句来源       Immunoassays with specific antibodies showed that rpS26 contained in the nuclear extract of HeLa cells binds to the first intron of its pre-mRNA and, less efficiently, to its mRNA. 例句来源       更多             intron 1 Male-specific DNA-protein complexes were revealed with labeled oligonucleotides corresponding to fragments +1150/+1449, +1358/+1449, +1397/+1449, and +1417/+1449 of intron 1 of the Ste1 gene. 例句来源       Intron 1 of Ste1 gene contains the repeated element of ID family inserted after duplication of the ancestor gene. 例句来源       This mRNA, which contained 225 bp of the intron 1 proximal region, was detected in HeLa cells by reverse transcription-polymerase chain reaction. 例句来源       Alternative transcripts containing intron 1 were also found in mouse and rat cells. 例句来源       RET exons 8, 10, 11, 13, 14, 15, and 16 and intron 1 along with the GFRA1 5'-UTR were screened by PCR and subsequent direct sequencing or RFLP analysis. 例句来源       更多

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