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一抗     
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  first antibody
     The optimized conditions for ELISA were: first antibody 1∶1 600, second antibody 1∶3 200, streptavidin HRP 0.5 mg/L.
     最佳工作条件为 ,一抗 1∶ 1 6 0 0、二抗 1∶ 32 0 0、链霉卵白素 -HRP0 .5mg/L。
短句来源
     To observe the expression of PGP 9.5 and S-100 protein (the neurogenic markers) in the patients with Hirschsprung's disease and their significance in clinical diagnosis , anti-PGP 9.5 and anti-S-100 protein were used as the first antibody to do the PAP immunohistochemical staining.
     为观察神经标志物PGP 9.5和S-100蛋白在先天性巨结肠(HD)中的表达,采用一抗为抗PGP 9.5和抗S-100蛋白的PAP免疫组织化学方法,探讨其在临床诊断中的意义。
短句来源
     One of the every 2 slices was used as negative control by replacing first antibody of STAT3 with 0.01 mol/L PBS.
     每2张切片中取1张用0.01mol/LPBS代替STAT3一抗设阴性对照实验。
短句来源
     The assay used chPRL(AFP\|10328B)as reference standard,chPRL(AFP\|4444B) as the radio labelled ligand,rabbit antichicken PRL(AFP\|151040789) as first antibody,and donkey anti\|rabbit IgG antiserum as second antibody.
     所用试剂为标准chPRL( AFP10328B) ,碘标chPRL( AFP4444B) ,一抗为免抗chPRL(AFP151040789) 抗血清,二抗驴抗免IgG 抗血清。
短句来源
     Results The constant and clear reaction was observed in the salivary gland tissues with the first antibody concentration of 1∶200 for AQP2 and 5, and 1∶100 for AQP1. But the reaction of AQP 3 and 4 were not found at the concentration of 1∶100, even 1∶50. AQP1 was labeled in blood capillary endothelium and basement membrane, and AQP2 in the alveolar basement membrane and secretary duct.
     结果 AQP2、AQP5为 1∶2 0 0和AQP1为 1∶10 0的一抗浓度时可以看到它们在腮腺、颌下腺、舌下腺、唇腺有稳定、清晰的染色反应 ,但AQP3、AQP4为 1∶10 0乃至 1∶5 0的一抗浓度也未观察到染色。 AQP1在各涎腺腺泡的基膜 ,毛细血管床及导管基膜等处被标记。
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  primary antibody
     MATERIALS:RPMI1640 complete medium,dexamethasone,β glycerophosphate,ascorbate,mouse anti human primary antibody,goat anti mouse secondary antibody,streptavidin(tertiary antibody),DAB(1∶50),100 mL/L of fetal calf serum,vitamin C,transforming growth factor β1(TGF β1),etc.
     材料:RPMI1640完全培养基,地塞米松,β-甘油磷酸钠,抗坏血酸,鼠抗人一抗,羊抗鼠二抗,链球菌亲和素(三抗),DAB(1:50),100mL/L胎牛血清,维生素C,转化生长因子-β1(TGF-β1)等。
短句来源
     The centrosome was stained by indirect immunofluorescence methods and the primary antibody is polyclonal antibody against pericentrin.
     中心体染色采用间接免疫荧光染色方法,一抗为抗中心体周围蛋白多克隆抗体(Polyclonal Antibody against Pericentrin)。
短句来源
     Methods The anti human blood type A,B,H monoclonal antibodies were used as the primary antibody, and the immunohistochemistry EnVision technique was used to detect the expression of A,B,H antigens in tissues from 10 pigs.
     方法抗人类血型A、B、H抗原单克隆抗体为一抗,免疫组织化学EnVision法分别检测10头中国内江猪多种组织人类血型A、B、H抗原分布。
短句来源
     A rabbit monoclonal antibody against rat IGF-1 (SANTA CRUZ Company, USA. 0.1ml, Diluted 1:200 before use) was used as the primary antibody in immunohistochemical staining.
     下丘脑、垂体和卵巢中的IGF-1的表达用IH方法测定,IGF-1免疫组化的一抗是兔抗大鼠IGF-1单克隆抗体(美国SANTA CRUZ公司生产,0.1ml,稀释1:200后应用),免疫组化结果判断标准:同一样本至少重复染色一次,由至少两位经验丰富的专业人员独立阅片。
短句来源
     ②1 μL antigen was spotted into the center of NC membrane and dried in the incubator at 37 ℃. Then the NC membrane was blocked in 1 mL PBST and put in the incubator at 37 ℃ with shaking in 90 r per minute for 15 minutes. Then the liquid was exchanged with 1 mL PBST and added the primary antibody at a final concentration of 10 mg/L.
     ②1μL抗原被点样在硝酸纤维素膜的中心,于37℃孵箱中烤干,浸于1mL的PBST中,37℃孵箱90r/min封闭振荡15min,重新更换1mL的PBST,加入一抗卵黄抗体,终浓度为10mg/L。
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  primary anti
     Instead of primary anti, we use normal primary anti animal blood serum as the negative contrast, the antibody is provided by Beijing zhongshan biotechnology company.
     以正常一抗动物血清代替一抗做阴性对照 ,抗体由北京中山生物技术公司提供。
短句来源
  primary antibodies
     2. ^Reagent: The primary antibodies p53 ( mouse monoclonal antibody, DO -7) and p63(mouse monoclonal antibody, 4A4 +63P02) ,SP kit and DAB developer were bought from Fuzhou Maixin.
     2.实验试剂:一抗p63(4A4+63P02)鼠抗人单克隆抗体、一抗P53(DO—7)单克隆抗体、SP试剂盒、DAB显色剂,均购自福州迈新公司;
短句来源
     In each immunostaining run,the two primary antibodies we replaced by PBS (0.01mol/L,pH7.4) as negative controls.
     去除2个一抗,设置PBS(0.01mol/L,pH7.4)为阴性埘照,其余步骤不变。
     Primary antibodies used in experimental groups are IgG from MG patients and NF-160 or P Tubulin;
     实验组分为两组,一抗用MG患者IgG分别与NF-160和β-Tubulin作双标;
短句来源
     and primary antibodies used in control groups are IgG from healthy people and NF-160 or P Tubulin.
     对照组也分为两组,一抗用正常人IgG分别与NF-160和β-Tubulin作双标。
短句来源
     Methods: Using 7 different types of primary antibodies and 2 tumor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining and compared their sensitivities and specificities.
     方法应用7种不同类型的一抗,2张不同肿瘤的组织芯片,同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色,比较敏感性和特异性。
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      first antibody
    Tests of the first antibody reaction showed that non-specific co-precipitation of radioactive insulin was less than 3.5 % and that addition of sodium chloride did not alter the standard insulin curve.
          
    Anti-GC monoclonal antibody was bound and then a second antibody labeled with peroxidase directed against the first antibody.
          
    IgM is the first antibody to be produced in a humoral immune response and is a major isotope of natural antibodies and may play an important role in innate immunity.
          
    The following antibodies were used as the first antibody; FGF2 and FGF7 for FGFs, FGFR1 and FGFR2 for FGFRs, NCAM, L1CAM, and N-cadherin for CAMs.
          
    Either by UV-irradiation or by incubation with glutaraldehyde, the first antibody was covalently crosslinked to TMV, and the second antibody to the first.
          
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      primary antibody
    Kinetics of primary antibody responses to sheep red blood cells in birds: a literature review and new data from great tits and E
          
    Measuring the primary antibody immune response to an injection with sheep red blood cells (SRBC) is a routine application in poultry science.
          
    p53 overexpression was detected by LSAB immunohistochemistry using DO-7 primary antibody.
          
    Serum immunoglobulins, complement components, and primary antibody responses to influenza vaccine were unchanged.
          
    Immunoblot analysis with anti-SA11 antiserum as the primary antibody verified the presence of VP7::RTB fusion protein in transformed potato tuber tissues.
          
    更多          
      primary anti
    Initiation of primary anti-vaccinia virus immunity in vivo
          
    m.) for 7 days as primary anti-rejection therapy had a significant reduction in histopathological evidence of allograft rejection on Day 7, compared to allograft controls.
          
    The primary anti-NP response in C57BL/6 and CBA mice was analyzed by isoelectric focusing.
          
    These cells were shown to enhance the primary anti-CRBC PFC response of whole normal spleen cells in a dose-dependent fashion.
          
    The cells were capable of eliciting primary anti-B16 immune responses in vitro in spleen cells from C57BL/6 mice.
          
    更多          
      primary antibodies
    Expression of mature core protein, envelope protein El and E2 was detected by Western blot using HCV patient sera as the primary antibodies.
          
    Using the sera from cured rats as the source of primary antibodies, immune responses induced by laser immunotherapy were observed in both cellular and molecular levels using histochemical assays and Western blot analysis.
          
    Immunoblot analysis with anti-CTB and anti-LF primary antibodies verified the synthesis and assembly of biologically active CTB-LF fusion protein oligomers in transformed plant tuber tissues.
          
    When the tissues were stained by the PAP method with anti-Su streptococcus antibody used as the primary antibodies, the common antigens were demonstrated in 10 (45.5%) of the 22.
          
    The primary antibodies were immobilized within a defined detection zone (test line) on the diagnostic nitrocellulose membrane.
          
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    Physical properties of three types of paddy soil collected from south Kiangsu province were studied.The shearing strength of paddy soils increases with the decrease ofmoisture content,especially below the lower plastic limit.Maximum compressibility ap-pears at the high moisture content and it falls sharply near the middle part of plasticityrange.As compared with the soils of heavy texture,the light paddy soils have a highercompressibility and lowering soil water content to a greater extent is necessary to reducetheir...

    Physical properties of three types of paddy soil collected from south Kiangsu province were studied.The shearing strength of paddy soils increases with the decrease ofmoisture content,especially below the lower plastic limit.Maximum compressibility ap-pears at the high moisture content and it falls sharply near the middle part of plasticityrange.As compared with the soils of heavy texture,the light paddy soils have a highercompressibility and lowering soil water content to a greater extent is necessary to reducetheir compression.The soil moisture causes shrinkage,especially for soils of heavy texture.Shrinkage makes the paddy soils more compact than mechanical pressure.It issuggested therefore that the optimum soil moisture for the fall cultivation ranges fromlower plastic limit to the medium plasticity and for the spring cultivation the optimummoisture content may be higher than in the fall.

    从水稻土的抗切强度,压缩性,收缩性与水分含量的关系作了初步研究和讨论。水稻土的抗切强度随着湿度的降低而增高,塑性下限以下增加更为强烈,不存在一般所谓湿度稍低于塑性下限有一抗机械强度较小的酥软结持阶段。土壤(原状土)的压缩性以湿度大时为最高,随着湿度的降低而变小,接近塑性范围的中部,有一明显的转折点,在这转折点以下(或塑性范围的下部),土壤实际的压缩性是较小的。与旱地土壤的变化规律不一致。原因是水稻土垒结状况和粘结的条件不同。质地轻的土壤比粘重的土壤压缩性大,其压缩性降低至较小程度需要降低的土壤湿度比粘重的土壤为多,但其收缩变紧的程度不强烈,粘重水稻土在湿度降低时发生强烈收缩,使土壤变紧;当土壤湿度在塑性以下时,收缩引起土壤紧实的程度比一般人为的机械压力还更大,只有当湿度较大时,才有较高的总孔隙度。根据这些特性,提出水稻土秋耕适宜的土壤湿度是:以塑性下限为底限,以塑性中部为上限,对质地较轻的土壤,上下限宜酌量降低,但其上限不一定在塑性以下。适宜春耕的湿度比秋耕可大一些,因为晒垡和水中耕作,可再疏散土壤。

    Antiphage serawere produced in rabbits against the phages of Bacillusthuringiensis. On the basis of cross-neutralization experiments with homologous and heterologous antisera, the ten phages were classified into four serological groups, termed LP1, LP4, LP7, and LP5. Group LP1 containedthree Phages, i.e., LP1, LP3 and PP3. Group LP4 contained two phages, i.e., LP4 and LP2. Group LP7 contained four phages, i.e., LP6, LP7, LP8 aod PP2. Group LP5 one phage, i.e., LP5. This classification was in accord with morphological...

    Antiphage serawere produced in rabbits against the phages of Bacillusthuringiensis. On the basis of cross-neutralization experiments with homologous and heterologous antisera, the ten phages were classified into four serological groups, termed LP1, LP4, LP7, and LP5. Group LP1 containedthree Phages, i.e., LP1, LP3 and PP3. Group LP4 contained two phages, i.e., LP4 and LP2. Group LP7 contained four phages, i.e., LP6, LP7, LP8 aod PP2. Group LP5 one phage, i.e., LP5. This classification was in accord with morphological one that was reported in the preceding paper.

    制备了十种苏芸金杆菌噬菌体的抗血清,测定了每一抗血清的K值,并根据抗血清交叉中和反应实验将它们分为四个不同的血清型:LP_1,LP_4,LP_7和LP_5型。LP_1型包括:LP_1,LP_3和PP_1;LP_4型包括LP_4和LP_2;LP_7型包括LP_6,LP_7,LP_8和PP_2;LP_5型仅包括LP_5。这种分类与根据噬菌斑形态所进行的分类基本上一致。

    In this paper, according to experience of Haichang and Tangshan earthquakes, the following problems are studied;1. Classification method of buildings damaged by earthquake;2. Parameters of influence on earthquake damage to R. C. single-story factory buildings and relationships between parameters and damage ranks;3. Calculating method of earthquake damage ranks of an existing R.C. single-story factory building.

    本文提出了一个划分钢筋混凝土排架厂房震害等级的方法。这个方法把厂房的震害分为三部分:一、抗侧力构件的震害;二、围护墙的震害;三、屋面系统的震害。划分厂房的震害等级时,先分别评定这三部分的震害,然后将它们加权进行综合得厂房的震害等级。文中根据海城和唐山地震中的200多个厂房震害实例,提出了影响上述三部分震害的参数,并研究了这些参数与震害等级的关系。最后给出了一个估计现有钢筋混凝土排架厂房遇到不同烈度地震时的震害等级的方法。

     
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