The Clinical & Experimental Research on the K-ras Gene Point Mutations and Monoclonal Antibodies of the Gene Expression Products P21 Protein in Pancreatic Adenocarcinoma
MethodsWe screened a monoclone P815 cell line by limiting dilution assay (LDA),and detected whether the cell line expresses gene P1A with RT PCR and DNA sequencing.
Using the specific monoclonal antibodyof c-src gene express product , pp60 ̄(c-src)(McAb 327),we studied immunohistochemically in this experiment35 cases of the esophageal squamous cell carcinomasand 22 adjacent carcinoma tissues.
CHO cells were transiently transfected with the individual construct by calcium phosphate precipitate method. Target protein expression in the lysate of the transfected cells were measured by ELISA and immunocytochemical staining, with HPV 18 L1 E6 and L1 E7 specific monoclonal antibodies.
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
After CNBr treatment, the antigenic epitope(s) for a flavivirus-common monoclone (NARMA 24) and those for NARMA 16 appeared to locate on different fragments, while the epitopes for other monoclones lost their antigenicities.
The three-dimensional structure of the antigen-binding fragment of a monoclonal antibody to human interleukin-2 was determined in two crystal forms by the X-ray method of molecular replacement at 2.2 and 2.9 ? resolutions.
The DNA fragmentation of thymocytes induced by MTSC4 was inhibited by the addition of 20 mmol/L N-acetyl galactosamin monosaccharide and the pretreatment of a monoclonal antibody (PF-18-3) which recognized a putative antigen on MTSC4.
Six species-specific monoclonal antibodies (mAbs) against the prM recombinant polypeptide recognized at least four epitopes on the recombinant polypeptides.
Using a highly specific monoclonal antibody against cardiac α-actin, we analyzed and compared the accumulation of this actin isoform in diverse SMC by immunofluorescence microscopy and immunoblotting.
IgG1, IgG2, IgG3 and IgG4 serum concentrations were determined in 49 phenytoin-treated epileptic patients and in 19 untreated epileptic controls, using subclass-specific monoclonal antibodies in an ELISA technique.
Circulating lymphocyte subpopulations defined by anti-CD45 and other more common T-cell-specific monoclonal antibodies were analysed in 77 patients with multiple sclerosis and 38 healthy controls.