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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
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Production and immunochemical characterization of monoclonal antibodies toCorynebacterium sepedonicum, the causative agent of po
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Five stable hybridoma lines producing monoclonal antibodies toCorynebacterium sepedonicum were obtained.
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The specificity of monoclonal antibodies obtained was characterized.
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Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1
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ER, PgR and monoclone M4G3 assays were performed for positive lymph nodes to confirm the primary foci to be breast cancer.
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Results: Some Vβ subfamily PCR products from 4 patients contained monopeak (monoclone) or a dominant peak (oligoclone).
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After CNBr treatment, the antigenic epitope(s) for a flavivirus-common monoclone (NARMA 24) and those for NARMA 16 appeared to locate on different fragments, while the epitopes for other monoclones lost their antigenicities.
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According to a multi-step hypothesis, the antigenic stimulation in a susceptible host is the first hit, giving rise to a benign monoclone.
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A monoclonE al antibody against this factor has been obtained.
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garcae strains by using a set of mouse monoclonal antibodies specific to the lipopolysaccharide O-polysaccharide.
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The clinical significance of HAMA in patients treated with mouse monoclonal antibodies
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Twenty-four patients were analyzed for the development of HAMA (human antimouse antibodies) after being treated with repeated doses (200-500 mg) of the mouse monoclonal antibody (MAb) 17-1A.
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Their variable heavy (VH) and light (VL) domains are derived from the mouse monoclonal antibody 215 (MAb215), specific for the largest subunit of RNA polymerase II ofDrosophila melanogaster and rat MAb Yol1/34, specific for pig brain α-tubulin.
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Omalizumab is a humanized mouse monoclonal antibody that binds specificially to the constant region of the immunoglobulin (Ig)E heavy chain.
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In this study, we describe the preparation of murine monoclonal antibodies (MAs) to TMV MP and mapping of the MP epitopes.
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An analysis of nerve growth factor (NGF) receptor expression and density in human sural nerve biopsies was performed by immunocytochemistry with a murine monoclonal antibody against the human NGF receptor.
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Murine monoclonal antibody (MoAb) BB4.3, raised against the human gastric cancer cell line BGC823, was puriffied with Protein A-Sepharose CL-4B affinity chromatography and identified as IgG2a.
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A murine monoclonal antibody HAb27 specific for human hepatocarcinoma has been developed for radio-immunolocalization in animal models.
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coli was used to express the variable domains of the murine monoclonal antibody HD39 specific for the human B-cell surface antigen CD22.
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| | Monoclonal antibodies against H241 strain of dengue-4 virus were obtained from lymphocyte hybri-domas produced by fusing immune mouse lymphocytes with mouse myeloma cells. Hybridoma cell culture supernatants were screened by indirect immunofluorescent antibody technique. Monoclonal antibodies in mouse ascitic fluid induced by five selected hybridomas were characterized also by the immuno-fluorescence test. Two hybridoma-producing antibodies were type-specific for dengue-4. Their titers were 1:10,240-1:20,48... | | 用登革4型H241株病毒免疫的小鼠脾细胞与小鼠骨髓瘤细胞融合,获得了5个产生抗登革4型病毒单克隆抗体的杂交瘤细胞系。这些杂交瘤细胞的上清液及小鼠腹水抗体均用间接免疫荧光法检测。其中两个杂交瘤细胞系产生的抗体对登革4型病毒具有型特异性,它们的荧光抗体滴度分别为1:10,240~1:20,480和1:2,560。另两个细胞系产生的抗体对登革2型病毒有交叉;另1个对登革1型病毒有交叉。 | | 文摘来源 | | We have established a hybridoma cell line (6B11) by fusion of mouse myeloma cells (NSI) and splenocytes from BALB/C mouse immunized with JEV-SA4. Hybrid clones that produced specific antibody were screened by ELISA, HI, and IP techniques, and 6B11 cell line has been found to be able to produce high liters of antibodies specific for JEV-SA4. This cell line (6B11) was cloned 4 times successively by limiting dilution method, and some subclones producing specific antibodies were obtained and stored in liquid ni... | | 用JEV-SA,免疫BALB/C小鼠的脾细胞与小鼠骨髓瘤细胞(NS_1)在PEG作用下融合,以ELISA、HI、IF 3种方法检测抗体,建立了1株针对JEV-SA_4的单克隆抗体杂交瘤细胞系。该细胞系染色体在74~98之间,含中着丝点标志染色体,经4次克隆化,连续传代5个月仍持续分泌特异性抗体,该抗体为IgG_3亚类。 | | 文摘来源 | | IgG and IgM was purified from immunized C57BL/6J mouse serum by means of the combination of several biochemical techniques. High-yeilding and high purity IgG1 and IgM of mouse could be obtained by seperating monoclonal antibodies with affinity chroma-tography and proteinA-Sepharose CL4B column. The Rabbit-anti-mouse IgG could be used as the reagent in the lymphocyte hybridization technique. | | 结合几种生化技术从C_(57)BL/6J小鼠分离得纯度好的IgG和IgM。由单克隆抗体经亲和层析柱和A蛋白—琼脂糖也分离得产量高、纯度好的小鼠IgG_1和IgM。用分离的IgG免疫得兔抗小鼠IgG血清可用为淋巴细胞杂交瘤技术中的试剂。 | | 文摘来源 | |   | | << 更多相关文摘 |
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