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      单克隆
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  monoclonal
    Bacterial Expression of Porcine Circovirus Type 2 Genes and Preparation of Monoclonal Antibody and Animal Experiment with Infectious Molecular Clone
    猪圆环病毒2型基因的原核表达与单克隆抗体的制备及感染性分子克隆的动物接种试验
短句来源
    EffectofCo-expressingChickenIL-2,IL-18,IFN-γ or Built CpG DNA in the Plasmid Backbone as Adjuvants on DNA Vaccines Against H5 Subtype Avian Influenza Delivered by Attenuated Salmonella and Production of Anti-chicken CD4 and CD8 Monoclonal Antibodies
    鸡IL-2、IL-18、IFN-γ和CpG DNA在减毒沙门氏菌运送H5亚型禽流感核酸疫苗中的佐剂作用及鸡CD4和CD8分子单克隆抗体的研制
短句来源
    Generation and Evaluation of Monoclonal Antibodies and Single-chain Antibody Against ICAM-1
    抗ICAM-1单克隆抗体与单链抗体的制备及其生物学特性研究
短句来源
    Study on Differential Diagnostic Assays of Porcine Contagious Pleuropneumonia and Monoclonal Antibodies Against Exotoxins of Actinobacillus Pleuropneumoniae
    猪传染性胸膜肺炎鉴别诊断方法和胸膜肺炎放线杆菌外毒素单克隆抗体研究
短句来源
    Monoclonal Antibody Technique
    单克隆抗体技术
短句来源
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  four monoclonal
    Methods Four monoclonal antibodies (M2, M3 M4, and M5) of Tyzzer's organism were used as ligands to screen antigenic epitope-associated peptides of Tyzzer's organism by biopanning a phage-display random peptide library (RPL) of 7 ammino-acid residues.
    方法选用泰泽氏病原体的四种单克隆抗体(M2、M3、M4、M5)作为配基,从噬菌体表面展示的随机7肽文库中筛选单抗识别的抗原表位,获得特异性噬菌体克隆;
短句来源
    Four monoclonal antibodies named 2A4、 B4、 F6 and H6 react with 25 H9N2 subtype strains from different areas of the whole nation between 1998 and 2005 by the hemagglutination-inhibition (HI)tests.
    根据我国当前禽流感H9亚型的变异和流行现状,结合国内外在HA的抗原性变异的研究情况,利用本文建立的H9亚型单克隆抗体2A4和河南农业大学禽病研究所研制保存的另三株单抗F6、H、B4,与1998~2005年间河南农大禽病研究所分离到的25个H9亚型禽流感毒株进行HI反应。
短句来源
    In this study four monoclonal antibodies designated as AC9, CF8, DG5 and EC9 against classical swine fever virus Shimen strain(CSFV-Shimen) were generated by immunization of Balb/C mice with purified CSFV- Shimen, All McAbs except DG5 could react with CSFV E2 protein in indirect ELISA, indicating that AC9, CF8 and EC9 are anti-CSFV E2 protein McAbs.
    本研究用猪瘟石门毒(CSFV-Shimen)免疫BALB/C小鼠,按常规单克隆抗体(McAb)技术方法制作,最终获得4株McAb,分别命名为AC9、CF8、DG5和EC9,4株McAb与基因工程CSFV E2蛋白反应结果表明:AC9、CF8和EC9是抗CSFv E2蛋白的McAb。
短句来源
    And four monoclonal antibody all could react with the AIV.Among them,ⅠF7 belongs to IgG2b,ⅠA3、ⅠB3、ⅠC4 belongs to IgG1.Their titers in cell culture supernatant were 1∶320,1∶160 and 1∶640 respectively,and titers in ascites were 1∶2×10~5,1∶5×10~4 and 1∶4×10~5 respectively.
    通过间接ELISA测定,单抗效价范围是:细胞培养上清1∶160~1∶640,腹水为1∶5×104~1∶4×105; 经ELISA法测定,四种单克隆抗体仅与试验的H7病毒株反应,而不能与禽流感H5亚型病毒、禽流感H9亚型病毒等反应;
短句来源
    The Balb/c female mice were injected subcutaneously the purified vesicular stomatitis virus four times, five monoclonal antibodies (McAbs) specific for VSV obtained by fusion of SP2/0 myeloma cells with splenocytes from BALB/c mice immunized with purified VSV, four monoclonal antibodies (McAbs) belongs to IgG2a, one monoclonal antibody belongs to IgM.
    纯化浓缩水泡性口炎病毒(VSV-IN)作为免疫原,免疫Balb/c小鼠,当免疫抗体效价达到融合要求时,在融合前3d尾静脉加强注射免疫原,取免疫的Balb/c小鼠的脾脏与SP2/0细胞在PEG4000作用下,融合、筛选、克隆获得5株稳定分泌抗VSV单克隆抗体的杂交瘤细胞株。
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  “单克隆”译为未确定词的双语例句
    Distinquishing Malleus from Melioidosis by Measuring Antibody with McAb
    应用单克隆抗体鉴别鼻疽和类鼻疽血清抗体的研究
短句来源
    Analysing the Antibody Component in Serum with McAb Technique
    应用单克隆抗体技术分析血清抗体成分的方法学研究——附56例鼻疽血清分析结果
短句来源
    THE APPLICATION OF McAb IN INFECTIOUS BURSAL DISEASE OF CHICKENS
    单克隆抗体在鸡传染性法氏囊病上的应用
短句来源
    PREPARATION OF RAHDV MONOCLONIC ANTIBODY
    抗兔病毒性出血症病毒单克隆抗体的制备
短句来源
    A REVERSE PASSIVE HEMAGGLUTINATION TEST FOR DETECTION OF RABBIT HEMORRHAGIC DISEASE VIRUS
    用单克隆抗体反向间接血凝检测兔出血症病毒
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  monoclonal
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
例句来源      
Production and immunochemical characterization of monoclonal antibodies toCorynebacterium sepedonicum, the causative agent of po
例句来源      
Five stable hybridoma lines producing monoclonal antibodies toCorynebacterium sepedonicum were obtained.
例句来源      
The specificity of monoclonal antibodies obtained was characterized.
例句来源      
Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1
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  four monoclonal
We report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitopes specific to GFAP.
例句来源      
Four monoclonal antibodies, directed against three different PSA epitopes, and one polyclonal anti-PSA antiserum were used.
例句来源      
An monoclonal HLA-A-, B-, and C-specific antibody and four monoclonal HLA-DR-specific antibodies cross-reacted with cells from all the primate species tested.
例句来源      
We have confirmed and extended these findings with four monoclonal antibodies that define a minimum of three separate class I specificities.
例句来源      
A matrix approach to human class II histocompatibility antigens: Reactions of four monoclonal antibodies with the products of ni
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         N-79 is a chicken embryo propagated vaccine derived from the LaSotastrain by cloning.Chickens received the vaccine in the drinking water at13—26 days of age.The dosage administered was 1 ml vaccine from allantoicfluid for 200 birds.The highest HI antibody level was obtained 14 days aftervaccination.Chickens challenged with the virulent strain of Newcastle diseasevirus demonstrated that the chickens with 3 log2 antiserum titer were pro-tected against the challenge,Chickens received the vaccine in the drinkin...
            单克隆(N-79型)是新城疫 LaSota 株经克隆纯化制成弱毒疫苗。应用鸡胚尿囊液的疫苗毒对13—26日龄的雏鸡经饮水免疫,1毫升疫苗免疫200只雏鸡,免疫14天后血清 HI 抗体滴度上升到高峰。免疫鸡经新城疫强毒毒株攻击试验证明,鸡体的血清 HI 抗体滴度在3 Log2就能抵抗新城疫强毒的感染。每毫升疫苗经饮水免疫500只雏鸡也可产生坚强的免疫力。这疫苗也可用于滴鼻免疫。这疫苗对雏鸡经2次饮水免疫(间隔4周),免疫期可达5个月以上。这疫苗在一个养鸡场使用时,在免疫后45天该场的鸡群受到新城疫强毒侵害,凡是用过新城疫 B_1和 LaSota 系疫苗免疫的鸡有一部分被感染死亡,而应用单克隆(N-79型)疫苗免疫的鸡没有死亡。
文摘来源
         Spleen cells from BALB/c mouse immunized with Bacillus anthracis protective antigen were fused with mouse myeloma cell line NS-1 by PEG 1000. Hybridoma cells producing special antibody were screened with radioimmunoassay method and subcloned by limiting dilution method. Then four hybridoma cell lines producing monoclonal antibody against B. anthracis protective antigen were obtained. By genome analysis, they were proved to be hybrid cells. They still produced antibodies constantly after culture in vitro for...
            用炭疽保护性抗原免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞NS-1融合,融合剂为PEG1000。用放射免疫方法筛选分泌特异抗体的杂交瘤细胞后,经有限稀释法及再克隆选择单克隆细胞,获4株能产生抗炭疽保护性抗原的单克隆抗体的杂交瘤细胞株。经染色体组型分析,证明是杂交细胞。体外连续培养8个月,仍能持续稳定地分泌抗体。其中,C17杂交细胞株,从它冰冻保存的第20代细胞复苏后,又传30多代,并接种在小鼠腹腔内增殖,仍能分泌特异性抗体。用正向间接血凝法测定其培养上清液及腹水,抗体滴度分别为1:256~512,1:4,096~6,144。
文摘来源
         Two hybridoma Cell lines, JLZK—7 and JLZK—10, secreting specific antibodies to K88 adhesin antigen of enteropathogenic E.coli from swine were derived from 2 separate fusions between mouse myeloma cells Sp_2/0—Ag14 and spleen cells from mice immunized with K88 adhesin antigen which was purified by Sephadex column chromatography. The antibodies from the culture medium have titers of up to 100 in the indirect immunofluorescence test, and those from mouse ascitic fluid have titers ranging from 10~4—10~6.As show...
            用等电点沉淀和葡聚糖凝胶柱层析法从大肠埃希氏菌C83901株(08:K87,K88_(ab))培养物分离和提纯K88_(ab)抗原,并以200ug/只的剂量免疫BALB/c纯系小鼠。第三次免疫后72小时取免疫小鼠脾脏淋巴细胞与小鼠骨髓瘤细胞SP_2/0—Ag14进行融合,得到能分泌抗K88_(ab)特异单克隆抗体的杂交瘤细胞系4个,分别命名为JLZK—1、JLZK—4、JLZK—7和JLZK—10。经多次克隆后,JLZK—7和JLZK—10在体外长期培养仍能稳定地分泌特异单克隆抗体。用JLZK—7和JLZK—10接种经降植烷(Pristane)激化的BALB/c小鼠所产腹水的免疫荧光滴度高达10~4—10~6,直接凝集反应滴度高达10~2—10~4。免疫荧光试验和直接凝集试验表明JLZK—7单克隆抗体能与所试验的含K88_(ab)或K88_(ac)抗原的全部大肠埃希氏菌反应,而不与K88抗原阴性的大肠杆菌和肠杆菌科的其他细菌起反应,是针对K88_(ab)抗原中成分a的型共同的单克隆抗体。另一方面,JLZK—10单克隆抗体只与大肠埃希氏茵中...
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