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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
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Production and immunochemical characterization of monoclonal antibodies toCorynebacterium sepedonicum, the causative agent of po
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Five stable hybridoma lines producing monoclonal antibodies toCorynebacterium sepedonicum were obtained.
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The specificity of monoclonal antibodies obtained was characterized.
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Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1
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| | The results of detecting type B botulinum toxin by ?ELISA-double sandwich system are reported with the application of monoclonal antibody against type B botulinum toxin as the coating antibody. The minimal detectable concentration of type B toxin was 16 ng/ml ( 4 LD50/ml), and the threshold of cross-reaction with type A botulinum toxin was 32ug/ml (300 LD50/ml ) with a specific index of 2000 or over. The mnimal detectable concentration of type B botulinum toxin contaminatded in foods was about C.67-7.8 LD50... | | 于ELISA—双夹心法系统中采用B型肉毒毒素单克隆抗体作为包被抗体,可检出B型肉毒类毒素4—31ng/ml,与A型交叉最低浓度为4—8μg/ml,特异性比值为250—1,000;可检出B型肉毒毒素16ng/ml(4LD_(50)/ml) A型大于32μg/ml(大于301LD_(50)/ml),特异性比值大于2,000;检出污染食物标本中的B型肉毒毒素最低浓度为0.67—7.8LD_(50)/ml,与A型间未见交叉现象。其敏感度接近RPHA,特异性明显优于RPHA。 | | 文摘来源 | | An ELISA method was established for screening monoclonal antigbodies on the surface of platelets and other cells, and was compared with Radioimmunoassay(RIA). 10ng/100ul level of anti-platelet antibody AN51 gave a positive reaction by ELISA method. In 100 samples of hybridoma supernatant, ELISA and RIA gave 55 and 57 positive results respectively, with no statistical difference (P>0.5). The rate of coincidence was 90%. OD value of ELISA and epm of RIA were eorelated(r=0.89). Compared to RIA, ELISA was safer... | | 本文介绍一种筛选针对血小板及其它细胞表面抗原的单克隆抗体的酶联免疫吸附法(ELISA)。本方法特异性与敏感性较高,10ng/100ul浓度的抗血小板单克隆抗体即呈明显的阳性反应。与放射免疫法(RIA)同时测定100份杂交瘤培养上清,结果ELISA法55份阳性,RIA法57份阳性,符合率为90%,无统计学差异(P=0.75)。ELISA法OD值与RIA法cpm值之间高度相关(r=0.89)。ELISA简单安全,抗原细胞用量为RIA的1/50,是一种实用的方法。 | | 文摘来源 | | The technique of Clq-ELISA has been established in this laboratory for the detection of antihuman Clq monoclonal antibodies(McAb). This technique, as verified by displacement test, blocking test, and cross titration test, possesses high specificity. It is more sensitive than double immunodiffusion as verified by the tests of 15 specimens of mouse antihuman Clq serum and has better reproducibility. It is concluded that this technique is very useful for the detection of anti-Clq McAb in the supernatant of hyb... | | 为检测抗人C1q单克隆抗体,我们建立了固相C1q-ELISA。经取代试验、阻断试验及交叉试验证明本法具有特异性;同时用C1q-ELISA及双向免疫扩散试验检测15份鼠抗人C1q血清抗体滴度,表明C1q-ELISA法较双向免疫扩散法高20156倍,用6份血清试验批内及批间的变异系数范围,分别为1.8~6.5%及6.2~16.5%,说明有较好的重现性。因而,本法适用于杂交瘤细胞上清中抗人C1q单克隆抗体的检测。 | | 文摘来源 | |   | | << 更多相关文摘 |
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