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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
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Production and immunochemical characterization of monoclonal antibodies toCorynebacterium sepedonicum, the causative agent of po
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Five stable hybridoma lines producing monoclonal antibodies toCorynebacterium sepedonicum were obtained.
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The specificity of monoclonal antibodies obtained was characterized.
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Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1
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ER, PgR and monoclone M4G3 assays were performed for positive lymph nodes to confirm the primary foci to be breast cancer.
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Results: Some Vβ subfamily PCR products from 4 patients contained monopeak (monoclone) or a dominant peak (oligoclone).
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After CNBr treatment, the antigenic epitope(s) for a flavivirus-common monoclone (NARMA 24) and those for NARMA 16 appeared to locate on different fragments, while the epitopes for other monoclones lost their antigenicities.
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According to a multi-step hypothesis, the antigenic stimulation in a susceptible host is the first hit, giving rise to a benign monoclone.
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A monoclonE al antibody against this factor has been obtained.
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| | Subpopulations of infiltrating mononuolear cells in 51 resected pulmonary carcinoma specimens were observed, using immunohistochemioal technique with monoclonal antibodies of OKT3, OKT4, OKT8, OKM1, B1 and Leu7. The majorconstituent of the infiltrating mononuolears was the T cell. Maorophages came next. B cells were less and K/NK cells the fewest. OKT3+ cells (T pan), OET4+ cells (T helper/inducer), OKT8+cells (T suppressor/cytotoxic) and OKM1+ cells (macrophages) infiltrated at the periphery of the tumor, ... | | 用单克隆抗体、卵白素生物素复合物法(ABC法),观察51例原发性肺癌组织中各种单核性细胞亚型的分布特点和胶原纤维反应情况。发现浸润的单核性细胞中T淋巴细胞最多,其次为单核巨噬细胞,两者除大量存在于肿瘤边缘部外,还见于肿瘤间质,乃至肿瘤实质中,与癌细胞直接接触。B淋巴细胞多集中成滤泡状,散在分布于肿瘤边缘及附近正常肺组织中。杀伤细胞/自然杀伤细胞(K/NK细胞)的数量极少。肿瘤周围胶原纤维反应程度与单核性细胞浸润程度相平行。作者认为二者都是机体肿瘤免疫的局部表现。 | | 文摘来源 | | A hybridoma from mouse myeloma cell line SP2/0(HGPRT-) and human peripheral lymphocytes.was obtained in the presence of PEG and selected in hypoxanthine-aminopterine-thymidine (HAT) medium.It was proved that 5-12 human chromosomes were retained in the hybrid population,with no interspecific chromosome translocation observed.Monocloned A4 population retained human chromosomes 9、11、X、14、18、19 and 22.The electrophoretic zymogram of G6PD in hybridoma shows a three-banded pattern, suggesting both parents specifi... | | 小鼠骨髓瘤细胞SP2/0(HGPRT-)与人外周血淋巴细胞经PEG介导融合,HAT培养基选择,获得了体外永久培养的人鼠杂交瘤NJ84。细胞遗传学分析证实杂种群体中尚保留5~12个人染色体,未查见种间染色体易位。A_4单克隆中保留着人9、11、X、14、18、19、22号染色体。杂种细胞G_6PD同工酶电泳出现三带型酶谱,证实人和鼠的G_6PD在杂种细胞中均有表达。同时观察比较了杂种及其亲本对细胞松弛素B的反应。这种人鼠杂交细胞可望用于人类基因定位、制备人类免疫球蛋白链基因克隆和人类单克隆抗体,也可用于恶性表达分析等方面的研究。 | | 文摘来源 | | Human acute granulocytic leukemia leucocytes and mouse myeloma Ag14—SP2/0(HGPRT~-) were used as parental cells for the fusion experiments in the presence of PEG. The cells were grown on the feed layer cells after being fused. Three hybrid clonse were selected and isolated in the HAT medium: XM—H—B_1, XM—H—A_2, and XM—H—D_2. Giemse—11 differential staining, G—banding, C—banding techniques and isozymes analysis of G6P—D on cellulose acetate membrane electrophoresis were used to identify the above clones. our ... | | 我们用急性粒细胞性白血病病人外周血白细胞和小鼠骨髓瘤Ag14—SP2/0细胞(HGPRT~-)为材料,以国产聚乙二醇(分子量10,000)为促融剂,杂交后在含有滋养层细胞的24孔培养板中经HAT培养液选择后获得三个杂交瘤细胞系:XM—H—B_1,XM—H—A_2和XM—H—D_2,经Giemsa—11分化染色,染色体G分带、C分带和G6P—D同功酶的醋酸纤维素薄膜电泳鉴定,证实为人鼠杂交瘤。值得提出的是杂种细胞的上清,经中国医学院科学院基础所测定:①对抗人全血出现沉淀线;②作SDS电泳——PAGE出现克隆带,分子量65,000万左右;③作各种人免疫球蛋白(包括IgG亚类)均阴性,但对一种抗人r血清出现沉淀线,其中XM—H—A_2有两条带。这次获得的三个杂交瘤细胞系说明我们正在建立的杂种细胞分布板是一个很好的材料,同时对于急性粒细胞白血病的发病机制和采用单克隆抗体技术诊断和治疗白血病研究提供了有效手段。 | | 文摘来源 | |   | | << 更多相关文摘 |
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