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脊髓灰质炎病毒pv
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  “脊髓灰质炎病毒(pv)”译为未确定词的双语例句
     We describe a simple method for the rapid detection and identification of poliovirus (PV) using one-step reverse transcription PCR (RT-PCR) method. Without RNA extraction, 20μL of mixed leagents for both RT and PCR were added into a tube,with sharing one buffer system. The whole procedure was performed with a single uninterrupted thermal cycling program.
     介绍一种逆转录─聚合酶链反应(RT-PCR)的简单快速检测和定型脊髓灰质炎病毒(PV)的方法,无需提取PVRNA,共用一种缓冲系统,将RT和PCR试剂混合在20μL反应体系中,整个操作可置于一不间断温度循环程度程序中完成。
短句来源
     The virus positive isolation rate was 790% for polio virus and 242% for enterovirus for AFP cases while the positive isolation rate was 242% for polio virus and 1212% for enterovirus from the contacts of AFP cases. Significant difference was found not only in the positive isolation rates for polio virus but also in those for enterovirus between the AFP cases and their contacts.
     AFP病例标本中脊髓灰质炎病毒(PV)分离率为7.19%,其他肠道病毒(EV)分离率为19.76%,而AFP病例接触者标本中,上述两指标分别为2.42%和12.12%,两类标本中同类指标间差异具有显著性(P均小于0.05)。
短句来源
     The RD, Hep-2 and L20B Cells were separately used for poliovirus isolation,identification and virus titration of 73 stool specimens and also for 64 other known viruses. The results shoed that L20B oh possesses very good specificity,it gave a 100% detedion rate ofpoliovirus in the known specimens and 13.7% in stool suspension, that were all higher than thatby RD and Hep-2 oh.
     应用RD、Hep-2、L20B细胞对64株已知病毒和73份粪便标本悬液进行脊髓灰质炎病毒(PV)分离鉴定及效价滴定.实验结果证明,L20B细胞具有很好的特异性,用该细胞检测已知病毒标本,PV检出率达100%;
短句来源
     Our study shoal that the sensitivities of RD, Hep-2 and L20B Cell lines are dilTerent. The false negative rate of ac oh to poliovirus was higher than the other two. L20B Cellshave a better sensitivity and specificity to poliothe, but they missed one strain of poliovirus.
     细胞的敏感性在脊髓灰质炎病毒(PV)检测中具有重要意义,运用RD、Hep-2及新引入的L20B细胞同时进行肠道病毒的分离及鉴定,结果3种细胞对PV出现了不同程度的漏检,RD细胞的漏检率较高.L20B细胞对PV具有较强的敏感性和特异性,但也出现了1株PV的漏检.不同细胞对PV检出率差异的原因有待于继续研究.
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  相似匹配句对
     campes-tris pv.
     pv.
短句来源
     pv.
     pv.
短句来源
     Polivirus ( PV ) is the main etiological a靍nt of paralytic poliomye-litis.
     脊髓灰质炎病毒(PV)是引起脊髓灰质炎的最主要的病毒。
短句来源
     POLIOVIRUS ENCEPHALITIS
     脊髓灰质炎病毒性脑炎
短句来源
     Molecular Evolution of Polioviruses
     脊髓灰质炎病毒的分子进化
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  poliovirus (pv)
In this study we looked for poliovirus (PV) persistence in the CSF of 20 patients with PPS, in a control group including 20 patients with unrelated neurological diseases, and in 7 patients with stable poliomyelitis sequelae.
      
The domain V within the internal ribosome entry segment (IRES) of poliovirus (PV) is expected to be important in its own neurovirulence because it contains an attenuating mutation in each of the Sabin vaccine strains.
      
Over the past 40?years, live oral poliovirus (PV) vaccines have contributed to the eradication of wild PV in most countries.
      
The translational control involving internal ribosome binding occurs in poliovirus (PV), human rhinoviruses (HRV), encephalomyocarditis virus (EMCV), foot-and-mouth disease virus (FMDV), and hepatitis A virus (HAV).
      
The host range of most poliovirus (PV) strains is restricted to simians.
      


Polivirus ( PV ) is the main etiological a靍nt of paralytic poliomye-litis. A recombinant of PV1 was identified during the molecular epide-miological survey by using sequence analysis of the genomic PV1/2A junc tion region ( residues 3296-3445) . The PVl isolates were tested at the beginning by Sabin 1-specific PCR and Dot hybridization and was ex-cluded from vaccine-related PV1. Its sequencing data of the 150 nucleotides shared 97% homology to Sabin 1. Expanded sequencing of its 5'-side re vcaled many nucleotide...

Polivirus ( PV ) is the main etiological a靍nt of paralytic poliomye-litis. A recombinant of PV1 was identified during the molecular epide-miological survey by using sequence analysis of the genomic PV1/2A junc tion region ( residues 3296-3445) . The PVl isolates were tested at the beginning by Sabin 1-specific PCR and Dot hybridization and was ex-cluded from vaccine-related PV1. Its sequencing data of the 150 nucleotides shared 97% homology to Sabin 1. Expanded sequencing of its 5'-side re vcaled many nucleotide substitutions identical to those of wild PV1 from residue3262 upward,which indicates the recombination site at resi-dues 3262/3263 junction. 14 of 57 PV1 isolates collected from 13 provinces or autonomous regions were found to be recombinant by a recombi-nant-specific PCR developed by us. The high sequence similarity among them suggests that these 14 PV1 isolates probably were the progeny of a recombinant produced by a patient dually infected by wild and Sabin 1. Studies of virulence, immunogenicity, transmissibility etc. of this recombinant are going on.

脊髓灰质炎病毒(PV)是引起脊髓灰质炎的最主要的病毒。我们在研究PV1野毒株的分子流行病学时,发现了一株PV1自然重组病毒。用Sabin 1特异PCR检定该毒株基因组2505~2601区段,或用两个Sabin 1特异RNA探针分别打点杂交检定641~740和2502~2575区段时,推断它可能为野毒株;而测定VP1/2A连接区段3296~3445序列时,又表明它为疫苗株。向5′端扩大测序,从3262起的5′侧有与我国PV1野毒株相同的许多核苷酸取代,证明它为野毒株和疫苗株的重组病毒,重组位点为3262/3263。实验中建立了检测该重组病毒的特异的PCR方法。检测我国10个省市、自治区47份PV1分离株中,5个省区14份麻痹病例由重组病毒感染引起。这些重组株在我们测序的3155~3445区段序列彼此几乎相同(同源性≥96.7%),推断它们可能是某病儿受野毒株、疫苗株双重感染产生重组病毒后的子代病毒。有关该重组病毒的毒力、免疫原性、传播性等正在进一步研究中。

The polymerase chain reaction (PCR),dot-hybridization with digoxigenin-labelled probe,neutralization test with monoclonal antibodies, plaque reduction, neutralization test Rct/40C marker characterization assay and D. marker characterization assay were applied for the intratypic differentiation of type I polioviruses isolated in Wenzhou of China. We compared the accordance rates of intratypic differential on diagnosis by the methods above,and analyzed the advantages and disadvantages of the methods used for the...

The polymerase chain reaction (PCR),dot-hybridization with digoxigenin-labelled probe,neutralization test with monoclonal antibodies, plaque reduction, neutralization test Rct/40C marker characterization assay and D. marker characterization assay were applied for the intratypic differentiation of type I polioviruses isolated in Wenzhou of China. We compared the accordance rates of intratypic differential on diagnosis by the methods above,and analyzed the advantages and disadvantages of the methods used for the diagnosis. This study is useful for the intratypic differentiation of wild and vaccine-associated strains of polioviruses in China.

将分子生物学方法(聚合酶链反应和Digoxigenin标记的寡核苷酸探针打点杂交)、免疫学方法(单克隆抗体中和试验和蚀斑减数中和试验)及生物学方法(病毒Rct/40℃特征和D特征测定)相结合,用于Ⅰ型脊髓灰质炎病毒(PV)型内差异的综合鉴别诊断。分析比较了上述各方法区别野毒株与疫苗相关株,计算符合率的优缺点,为脊髓灰质炎病毒野毒株与疫苗相关株的鉴别诊断提供了依据。

We describe a simple method for the rapid detection and identification of poliovirus (PV) using one-step reverse transcription PCR (RT-PCR) method. Without RNA extraction, 20μL of mixed leagents for both RT and PCR were added into a tube,with sharing one buffer system. The whole procedure was performed with a single uninterrupted thermal cycling program. A good concordance was found among the PV neutralijation test, the routine RT-PCR and one-step RT-PCR. The sensitivity of one-step RT-PCR method reached the...

We describe a simple method for the rapid detection and identification of poliovirus (PV) using one-step reverse transcription PCR (RT-PCR) method. Without RNA extraction, 20μL of mixed leagents for both RT and PCR were added into a tube,with sharing one buffer system. The whole procedure was performed with a single uninterrupted thermal cycling program. A good concordance was found among the PV neutralijation test, the routine RT-PCR and one-step RT-PCR. The sensitivity of one-step RT-PCR method reached the virus infectious titer of STCID50/mL. The needed time, cost and opportunity of contamination of this assay was reduced greatly. The one-step RT-PCR method is especially suitable for routine detection and identification of PV in clinical laboratories.

介绍一种逆转录─聚合酶链反应(RT-PCR)的简单快速检测和定型脊髓灰质炎病毒(PV)的方法,无需提取PVRNA,共用一种缓冲系统,将RT和PCR试剂混合在20μL反应体系中,整个操作可置于一不间断温度循环程度程序中完成。一步法RT-PCR与常规RT-PCR及中和抗体试验对PV的检测结果完全一致,它的检测敏感度可达5TCID(50)/mL。该法需要的时间,费用和污染的都大大减少,而且特别适于临床常规诊断和鉴别PV的需要。

 
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