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心血管系统疾病
内分泌腺及全身性疾病
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蛋白
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  protein 140
     Objective:To study the effect of grape procyanidin (GPC) on the levels of plasmaα-granule membrance protein 140 (GMP140) and thromboxane B2 (TXB2 ) in rats.
     目的:观察葡多酚(GPC)对大鼠血浆α颗粒膜蛋白140(GMP140)和血栓素B2(TXB2)的影响。
短句来源
     Objective: To study the changes and their significances of Granule Membrand Protein 140 ( GMPi40 ) > thromboxane B2 ( TXB2 ) , 6-Keto-prostaglandin-1 (6-keto-PGF i a ) plasma levels of dog models with carotid atherosclerosis.
     目的:探讨颈动脉硬化模型犬血浆α-颗粒膜蛋白140(Granule Membrand Portein140,GMP140)、血栓烷B_2(Thromboxane B_2,TXB_2),6-酮-前列腺素F_(1α)(6-Keto-Prostaglandin1_α,6-Keto-PGF_(11α))水平变化及其意义。
短句来源
     Methods:AntithrombinⅢ (ATⅢ),platelet granulate membrane protein140 (GMP140),von Willebrand factor(vWF),fibrinogen (Fg),tissueplasminogen activator (tPA),plasminogen activator inhibitor (PAI),plasminogen (Plg) and α2plasmin inhibitor (α2PI) were measured in 28 consecutive patients (APACHE Ⅱ≥12) suffering from acute severe cholangitis within 24 hours after emergency admission.
     方法:对28例急诊入院且APACHEⅡ评分≥12分的重症胆管炎患者立刻采血,分别测定抗凝血酶Ⅲ(ATⅢ)、血小板颗粒膜蛋白140(GMP140)、血管性假性血友病因子(vWF)、纤维蛋白原(Fg)、组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制剂(PAI)、纤溶酶原(Plg)及α2纤溶酶抑制剂(α2PI)。
短句来源
     Granule membrane protein 140( GMP - 140) is a granule membrane glyco-protein which exists on the a - granule membrane of quiescent platelete , and expressed on the surface of platelete membrane when the platelet is activated.
     血小板颗粒膜蛋白140(Granule membrane protein 140,GMP-140),也称P-选择素,是存在于静止血小板α颗粒膜上的膜糖蛋白,它随血小板活化反应而发生移位并表达在血小板膜上,是活化血小板的特异性标志物之一。
短句来源
     Platelet count, a granule membrane protein 140 (GMP 140) concentrations and activity of Factor Ⅹ(FⅩa) and antithrombin Ⅲ(AT Ⅲa) were determined before CPB, at 60 minutes of CPB, at 30 and 60 minutes after protamine administration, at first 12 hour of CPB, respectively.
     分别在CPB前、CPB6 0min、中和后 30min和 6 0min ,及手术后 1 2h测定血小板计数 (PLC) ,血浆α颗粒膜蛋白 1 4 0 (GMP 1 4 0 )的浓度 ,及血浆凝血因子Ⅹ (FⅩa)和抗凝血酶Ⅲ (AT Ⅲa)活性。
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  “蛋白140”译为未确定词的双语例句
     The levels of plasma GMP 140 before treatment in the CHD group[(29.34±5.36) μg/L] was obviously higher than that in the normal control group[(12.08±4.85) μg/L,P< 0.01],it was decreased insignificantly[(25.53±6.51) μg/L].
     冠心病患者超声治疗前血浆α颗粒膜蛋白140含量犤(29.34±5.36)μg/L犦明显高于正常对照组犤(12.08±4.85)μg/L犦(P<0.01),治疗后有所下降犤(25.53±6.51)μg/L犦,但无显著性意义。
短句来源
     1Comparison of the content of GMP-140 in the two groups: It was significantly higher in the diabetic group than that in the control group (28.8 ±10.3),(9.1±5.6)μg/L(t=2.37,P < 0.01).
     ①两组观察对象血小板α颗粒膜蛋白140含量的比较:糖尿病组显著高于对照组眼(28.8±10.3),穴9.1±5.6雪μg/L(t=2.37,P<0.01)演。
短句来源
     The Study of the Changes and Their Significances of GMP140、TXB2、6-keto-PGF1α Plasma Concentrations of Dog Models with Carotid Atherosclerosis
     颈动脉硬化模型犬血浆血小板α-颗粒膜蛋白140、血栓烷B2、6-酮-前列腺素F1α浓度的动态研究
短句来源
     Clinical Significance of Plasmaα-Granule Membrane Protein-140 in Blood Stasis of Primary Glomerular Disease
     血浆α-颗粒膜蛋白140测定在原发性肾小球疾病血瘀证中的临床意义
短句来源
     Effect of external therapeutic ultrasound on plasma GMP-140,TxB_2 of rats
     体外治疗性超声对大鼠血浆颗粒膜蛋白140和血栓素B_2的影响
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  相似匹配句对
     140 pour son amplitude.
     140;
短句来源
     RADIOIMMUNOASSAY OF F PROTEIN
     F蛋白的放射免疫分析
短句来源
     ALBUMINOUS CELLS
     蛋白细胞
短句来源
     Research on the expression of platelet granule membrane glycoprotein CD62P in pregnant women
     孕妇血小板α-颗粒膜蛋白-140的表达
短句来源
     The molecular weights of major proteins were between 32 KD and 140 KD.
     主要蛋白组份分布在32—140KD范围之间。
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  protein 140
The results showed that the platelet granule membrane protein 140 (GMP-140) level increased obviously in CHD groups compared with normal control, suggesting that platelet activation existed in CHD.
      
Localisation of receptor interacting protein140 (RIP140) within 100kb of D21S13 on 21q11, a gene-poor region of the human geno
      
Here, we report the mapping of the gene for receptor interacting protein140 (RIP140) on 21q11 by means of YACs, PACs and hybrid cell lines.
      


Owing to the deficiency of lysine in zein, the hydrolysate of this protein was used as the main source of amino acids in the preparation of the medium for microbiological estimation of lysine using Lcuconostoc mcsentcroides P-60. as the test ing microorganisms. Suitable quantities of tryptophane, arginine, cystine, glycine and serine were used to supplement the medium.The maximum rate of acid production by the microorganism in this medium is much higher than that in the medium used by Horn et al for the same...

Owing to the deficiency of lysine in zein, the hydrolysate of this protein was used as the main source of amino acids in the preparation of the medium for microbiological estimation of lysine using Lcuconostoc mcsentcroides P-60. as the test ing microorganisms. Suitable quantities of tryptophane, arginine, cystine, glycine and serine were used to supplement the medium.The maximum rate of acid production by the microorganism in this medium is much higher than that in the medium used by Horn et al for the same purpose. The latter medium shows a rapid increase of acid production up to 80 μg lysine per innoculated test, while our new medium gives a continuous increase of acid production up to 120μg. Thus, the range in which the amino acid can be estimated in much extended.Parallel analysis was made on 25 food samples with our new medium as well as those published in the literature. In 11 cases, the agreement was within 10%; in 13 cases, the results obtained with' our new medium were about 10% higher; and in only 1 case, the new medium gave a lower result. These differences are not due to routine analytical error as repeated estimation gave similar results.

本文介绍微生物法测定赖氨酸所用的一种经济培养基。其大部分氨基酸系由玉米胶蛋白水解物供给。补充的氨基酸为精氨酸、甘氨酸、色氨酸、胱氨酸和丝氨酸。 实验结果证明,用玉米胶蛋白作培养基,以测定食物中赖氨酸的含量时,可获得较稳定的结果和满意的收回率。用本培养基测定一般食物中赖氨酸的含量与用其他培养基测定的结果符合,故本培养基可以用于食物中赖氨酸的测定。

(1)The present communication is a continuation of previous study of drugs on the pathologico-physiological reactions of the infected rabbits.The following indices of the reactions were used,namely:(1) erythrocyte sedimentation rate (ESR),(2) plasma fibrinogen content,(3) plasma gamma globulin content,(4) prothrombin time and (5)body weight. (2)It was shown that a full course of potassium antimony tartrate (PAT) could depress the abnormal rise of ESR of the infected rabbit.The effect was more striking in the...

(1)The present communication is a continuation of previous study of drugs on the pathologico-physiological reactions of the infected rabbits.The following indices of the reactions were used,namely:(1) erythrocyte sedimentation rate (ESR),(2) plasma fibrinogen content,(3) plasma gamma globulin content,(4) prothrombin time and (5)body weight. (2)It was shown that a full course of potassium antimony tartrate (PAT) could depress the abnormal rise of ESR of the infected rabbit.The effect was more striking in the early (i.e.7th day after inoculation) than the late treatment (i.e.33rd day after inoculation).This effect of PAT on the ESR was found to be in parallelism with its therapeutic effect.This method may be utilized for evaluation of drugs against schistosomiasis. (3)Neither PAT nor strychnine had any effect on the ESR of the normal rabbit.Strychnine was also found to have no significant effect on the ESR of the infected rabbit,but it could markedly modify the inhibitory effect of the PAT. (4)The therapeutic effect of half-course of PAT was found to be about 10—20% less marked than that of combined treatment with strychnine. Strychnine alone did not show any therapeutic effect.It was also found that the dose of strychnine used in our experiment did not increase the toxicity of PAT as shown by mice toxicity test. (5)The therapeutic dose of PAT showed no effect on the plasma fibrinogen content of the normal rabbit but it could bring the increased plasma fibrinogen content back to normal in the infected animal. (6)After a course of PAT in the infected animal,the double peak rise of the palsma gamma globulin content was distinctly suppressed as compared with the control group.On the contrary,the new drug,1:7-Bis (p-dimethylaminophenoxy) heptane (APH) did not show the same effect, probably indicating its weak action against schistosomiasis. (7)The body weight of the PAT treated group was found to be much higher than the non-treated group,However,the group treated with APH showed a continuous drop of body weight. (8)It has been found,as by others,that the APH showed high toxicity and low therapeutic effect in the experimental animals.From the fact that APH showed pronounced effect on ESR and plasma fibrinogen content of the infected animal,one could not yet decide,with the available data,whether these effects are due to its action on the schistosome or its eggs,or to its toxic action on the host.

(一)本文报告了药物对于血吸虫病病兔及正常家兔的红血球沉降率,血浆纤维蛋白,丙种球蛋白,凝血酶元时值及体重的影响。(二)酒石酸锑钾的治疗,无论在家兔患病的早期或后期,均可抑制其血沉的加速,而以早期治疗的抑制作用更为显著。同时这个抑制血沉的作用与治疗后的成虫发育率是符合的。因此利用这种方法,可以考虑作为研究一些治疗血吸虫病药物的疗效指标。(三)酒石酸锑钾及士的宁本身对正常家兔的血沉并不引起改变,士的宁对于病兔的血沉也无明显作用,但士的宁可以影响酒石酸锑钾对病兔血沉的抑制作用。 (四)半疗程剂量酒石酸锑钾并用士的宁之疗效较单独应用半疗程剂量酒石酸锑钾者为高(10—20%),而单独应用士的宁并无任何疗效,轻度激动量士的宁也不增加酒石酸锑钾的毒性。(五)酒石酸锑钾的治疗剂量对于正常家兔之血浆纤维蛋白元无明显影响,但能使病兔增高之血浆纤维蛋白元回复正常。(六)酒石酸锑钾的治疗可以使病兔的丙种球蛋白在一定时期内保持在接近正常值范围内,这一抑制作用与感染对照组相比,甚为明显,而氨苯氧烷却无此作用,这一点可能与疗效是有关系的。(七)酒石酸锑钾治疗后的病兔之体重较感染...

(一)本文报告了药物对于血吸虫病病兔及正常家兔的红血球沉降率,血浆纤维蛋白,丙种球蛋白,凝血酶元时值及体重的影响。(二)酒石酸锑钾的治疗,无论在家兔患病的早期或后期,均可抑制其血沉的加速,而以早期治疗的抑制作用更为显著。同时这个抑制血沉的作用与治疗后的成虫发育率是符合的。因此利用这种方法,可以考虑作为研究一些治疗血吸虫病药物的疗效指标。(三)酒石酸锑钾及士的宁本身对正常家兔的血沉并不引起改变,士的宁对于病兔的血沉也无明显作用,但士的宁可以影响酒石酸锑钾对病兔血沉的抑制作用。 (四)半疗程剂量酒石酸锑钾并用士的宁之疗效较单独应用半疗程剂量酒石酸锑钾者为高(10—20%),而单独应用士的宁并无任何疗效,轻度激动量士的宁也不增加酒石酸锑钾的毒性。(五)酒石酸锑钾的治疗剂量对于正常家兔之血浆纤维蛋白元无明显影响,但能使病兔增高之血浆纤维蛋白元回复正常。(六)酒石酸锑钾的治疗可以使病兔的丙种球蛋白在一定时期内保持在接近正常值范围内,这一抑制作用与感染对照组相比,甚为明显,而氨苯氧烷却无此作用,这一点可能与疗效是有关系的。(七)酒石酸锑钾治疗后的病兔之体重较感染对照组病兔显著增高,而经氨苯氧烷治疗后之病兔,其体重仍日见减轻。(八)氨苯氧烷有较高的毒性,但疗效甚低,它对病兔的血沉及纤维蛋白元所出现的抑制作用,究竟属于对血吸虫或其虫卵的作用,抑系对于病兔机体的毒性作用,目前还不能肯定。

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶胰蛋白酶及结晶胰凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

 
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