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大鼠移植肝
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  rat liver graft
     Gene Transfer of huCTLA4-Ig to Induce Immune Tolerance of Rat Liver Graft
     基因转移huCTLA4-Ig诱导大鼠移植肝免疫耐受的实验研究
短句来源
     Change of transforming growth factor-IS expression in the bUiary epithelial cells of rat liver graft
     大鼠移植肝内胆管上皮细胞转化生长因子-β表达的变化
短句来源
     Conclusions NTG, a nitric oxide donor, attenuates the preservation reperfusion injury of rat liver graft through the NO/cGMP pathway.
     结论 一氧化氮供体药NTG通过NO/cGMP途径减轻大鼠移植肝保存再灌注损伤。
短句来源
     Objective To investigate the protective effect and mechanisms of NF-κB decoy oli- godeoxynucleotides(ODNs)on rat liver graft following ischemia-reperfusion injury.
     目的探讨抑制枯否(Kupffer)细胞核因子κB(Nuclear factor-kappaB,NF-κB)活性对减轻大鼠移植肝缺血/再灌注损伤(IRI)的作用和机制。 方法建立大鼠肝移植缺血/再灌注损伤模型。
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     Objective To observe the effect of ischemic postconditioning on apoptosis of hepatocytes and expression of caspase-3 gene protein of rat liver graft,and to elucidate the possible mechanisms.
     目的:观察缺血后处理(ischemic postconditioning,Post-con)对大鼠移植肝细胞凋亡及 Caspase-3蛋白表 达的影响,探讨在体内条件下,缺血后处理对大鼠移植肝脏凋亡的保护机制。
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  “大鼠移植肝”译为未确定词的双语例句
     Results CD4~+CD25~+ Tr cells developed significantly greater in the TOL group than in the REJ group.
     结果自发耐受组大鼠移植肝内CD4+CD25+Tr细胞含量显著高于急性排斥组。
短句来源
     The Construction of Ad.VSG-hBCL-2 and Its Protective Effect on Grafts of Rat Liver Transplantation Against Ischemia Reperfusion Injury
     Ad.VSG-hBCL-2的构建及其在大鼠移植肝缺血再灌注损伤中的保护作用
短句来源
     The Relationship between ATP Contents of Graft Liver and Early Function of Donor Liver after Transplantation
     大鼠移植肝中ATP含量与术后早期功能的关系
短句来源
     Induced differentiation of bone marrow stem cells in transplanted rat liver
     骨髓干细胞在大鼠移植肝中的诱导分化
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     ObjectiveTo observe the expression of iNOS in the transplanted liver tissue and the effec t of aminoguanidine/FK506 on acute rejection following liver transplantation.
     目的 观察大鼠移植肝组织中一氧化氮合酶 (iNOS)的表达及iNOS抑制剂氨基胍和免疫抑制剂他克莫司 (FK50 6)对肝移植术后急性排斥反应的影响。
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  相似匹配句对
     Anesthesia in Rat Orthotopic Liver Transplantation
     大鼠移植的麻醉
短句来源
     Lebertransplantation
     移植
短句来源
     Prevention of Complications of Liver Transplantation in Rats
     大鼠移植并发症的预防
短句来源
     Auxiliary partial orthotopic liver transplantation in rats
     大鼠原位辅助性部分移植
短句来源
     ONE HUNDRRD ORTHOTOPIC LIVER TRANSPLANTATIONS IN RATS
     100例大鼠原位移植
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  rat liver graft
Time-dependent changes in the viability of rat liver graft during cold preservation with Euro-Collins solution were evaluated with NADH fluorometry.
      
High-Na + low-K + UW cold storage solution reduces reperfusion injuries of the rat liver graft
      
The effects of vascular bed expansion in steatotic rat liver graft viability
      
The beneficial effect of superoxide dismutase on the rat liver graft
      
These results indicate the beneficial effect of SOD on the rat liver graft and may implicate oxygen free radicals in the pathogenesis of ischemia/reperfusion injury in liver grafts.
      


This stduy was aimed to investigate the effects of intrathymically transplanted donor splenocytes on the establishment of donor-specific unresponsiveness to the hepatic allografts. Male TJR/1 and SD rats were used as liver donor and recipients respectively. Isolated splenocytes from TJR/1 rat spleen were inoculated into the thymus of 8 SD rats pretreated with oral cyclosporine 40 rng/kg daily for 2 days and given 2- 5mg/kg dexamethasone subcutaneously at inoculation. Ten days later the liver from the TJR/1 donor...

This stduy was aimed to investigate the effects of intrathymically transplanted donor splenocytes on the establishment of donor-specific unresponsiveness to the hepatic allografts. Male TJR/1 and SD rats were used as liver donor and recipients respectively. Isolated splenocytes from TJR/1 rat spleen were inoculated into the thymus of 8 SD rats pretreated with oral cyclosporine 40 rng/kg daily for 2 days and given 2- 5mg/kg dexamethasone subcutaneously at inoculation. Ten days later the liver from the TJR/1 donor used to provide spleen cells was transplanted orthotopically to the SD rat that had received intrathymic splenocytes. Donor-specific unresponsiveness allowed 6 to 8 hepatic allografts to survive indefinitely without further immunosupression. Eight control SD rats treated without in-trathymical splenocyte transplantation rejected their hepatic allografts at 9+1. 15 days post operative-ly. All the rats in another control group intrathymically inoculated with third party spleen cells died at 8. 13 + 2. 23 days after liver transplantation. These results showed that the pretreatment with intrathymic splenocyte inoculation could induce donor-specific immunologic unresponsiveness to hepatic allograft and make it possible for the hepatic allograft to survive indefinitely without immunosup-pressants.

探讨大鼠胸腺内移植供体脾细胞在建立特异性供体不反应性中的作用。TJR/1大鼠作供体,SD大鼠为受体。切取供体鼠脾脏制成睥细胞悬液,注入到受体鼠胸腺内,此前两天受体鼠口服环孢素40mg·kg~(-1)·d~(-1),胸腺内脾细胞移植前皮下注射地塞米松25mg/kg。10天后取同一供体肝脏进行原位肝移植,术后不用免疫抑制剂。处理组半数以上大鼠存活超过100天,对照组存活9±1.51天。胸腺内移植了第3品系大鼠脾细胞的受体鼠,在接受了JR/1大鼠的移植肝后也短期死亡。表明这种供体不反应性是特异的。组织病理学及超微结构也证实脾细胞预处理的移植肝受损轻,排斥反应不明显。本研究提示了肝移植抗排斥反应的新途径。

Objectives To measure tumor necrosis factor α (TNFα) in rats on the 3rd, 6rd days after orthotopic rat liver transplantation, and detect the expression of TNFα mRNA in the grafts. Method Reverse transcription polymerase chain reaction (RT PCR) was used. Results The TNFα serum levels on postoperative day 3, 6 were significantly higher in allograft group than in isogeneic graft group. RT PCR analysis revealed that TNFα mRNA was present in allografts, while no TNFα mRNA expression occurred in isogeneic...

Objectives To measure tumor necrosis factor α (TNFα) in rats on the 3rd, 6rd days after orthotopic rat liver transplantation, and detect the expression of TNFα mRNA in the grafts. Method Reverse transcription polymerase chain reaction (RT PCR) was used. Results The TNFα serum levels on postoperative day 3, 6 were significantly higher in allograft group than in isogeneic graft group. RT PCR analysis revealed that TNFα mRNA was present in allografts, while no TNFα mRNA expression occurred in isogeneic grafts. Our findings supported that TNFα plays an important role in the pathogenesis of acute allograft rejection, and suggested that sequential determinations of TNFα after operation may be of value in the prediction and diagnosis of acute rejection.

测定原位肝移植大鼠术后血清肿瘤坏死因子α(TNFα)浓度,探讨其在原位肝移植急性排斥反应中的作用。方法利用RT-PCR检测大鼠移植肝组织内TNFαmRNA的表达,并观察移植肝病理形态变化。结果术后3、6天异基因大鼠血清TNFα浓度都显著高于同期同基因肝移植大鼠和正常对照鼠血清TNFα浓度。在异基因原位肝移植大鼠术后第6天,肝标本中检测到TNFαmRNA表达,而在同基因原位肝移植大鼠同期肝标本和正常大鼠肝标本中均未检测到TNFαmRNA的表达。结论TNFα在排斥反应中发挥重要作用,术后监测TNFα浓度变化对急性排斥反应的发生有一定预测和早期诊断作用。

Objective To investigate the effects of nitroglycerin (NTG), a nitric oxide donor, on preservation reperfusion injury of rat liver graft. Methods The graft was washed and preserved with lactated Ringer′s solution containing or not containing 10 μg/ml NTG. Then the following parameters were determined: 1) The contents of aspartate amino transferase (AST), alanine amino transferase (ALT) and lactic dehydrogenase (LDH) in the recipient serum at 10 min and 2 h after reperfusion. 2) The bile output at the 1st...

Objective To investigate the effects of nitroglycerin (NTG), a nitric oxide donor, on preservation reperfusion injury of rat liver graft. Methods The graft was washed and preserved with lactated Ringer′s solution containing or not containing 10 μg/ml NTG. Then the following parameters were determined: 1) The contents of aspartate amino transferase (AST), alanine amino transferase (ALT) and lactic dehydrogenase (LDH) in the recipient serum at 10 min and 2 h after reperfusion. 2) The bile output at the 1st and 2nd h after reperfusion. 3) The cyclic guanosine monophosphate (cGMP) of the liver preserved for 4 h and reperfused for 2 h. 4) The survival rate of over 1 and 7 d. Results 1) The levels of AST and LDH were significantly lower in NTG group than in the control after reperfusion for 10 min. 2) The bile output was markedly higher in NTG group than in the control ( P<0 05 & 0 01). 3) After preservation for 4 h and reperfusion for 2 h, the cGMP level was remarkably higher in NTG group than in the control (P<0 01). 4) One week survival rate was significantly higher in NTG group than in the control (P<0 01). Conclusions NTG, a nitric oxide donor, attenuates the preservation reperfusion injury of rat liver graft through the NO/cGMP pathway.

目的 研究一氧化氮供体药硝酸甘油 (NTG)对大鼠移植肝再灌注损伤的影响。方法 用 0~ 4℃含或不含NTG 10 μg/ml的乳酸林格液灌洗及保存肝脏 4h后行原位肝移植 ,观察再灌注10min ,2h受体鼠血清氨基丙酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、乳酸脱氢酶 (LDH) ;再灌注 1h ,2h的胆汁分泌量 ;再灌注 2h肝组织环磷酸鸟苷 (cGMP)含量 ;大于 1d和 7d的大鼠生存率。结果 再灌注 10min血清AST ,LDH对照组明显高于实验组 (P <0 0 5 ) ;再灌注 1h ,2h的胆汁分泌量两组间差异有显著意义 (分别为P <0 0 5 ,<0 0 1) ;经NTG保存 4h及再灌注 2h的肝组织cGMP含量比对照组增高 (P <0 0 1) ,1周生存率NTG组比对照组增高 ( 6 2 .5 %vs 0 % ,P =0 0 13)。结论 一氧化氮供体药NTG通过NO/cGMP途径减轻大鼠移植肝保存再灌注损伤。

 
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