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  level expression
     Conclusion These results suggest there is high level expression of P-selectin in patients with acute asthma,the level is negatively correlated with its pulmonary function, P-selectin has a potent effect on pathogenesis of asthma.
     结论 哮喘急性发作患者有P- selectin的高表达,表达水平与肺功能成负相关,P- selectin在哮喘的发病中起着一定的作用。
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     Conclusion:The anti CD3/anti CD20 BsF(ab') 2 was first recast into the Diabody format and succeeded to obtain high level expression. The results of some biological activity experiments indicated that the Diabody could bind to Jurkat cells and Daudi cells.
     结论 :利用Diabody形式 ,首次成功地构建了抗CD3/抗CD2 0微型双功能抗体 ,并获得较高表达 ,表达产物具有与相应 2个靶抗原结合的活性
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     The kanamycin resistance gene, which as the marker gene, was also subcloned into this expression system to obtain the expression vector pEUTMT 1. Induced by 42℃ heat shock for 30 min, the level of UB Tα 1 protein reached a value of 8.7% of the total soluble protein in the transformants of Synechococcus sp. PCC7942. The result of the experiment demonstrates that the heat shock induced high level expression system of Synechococcus sp. PCC7942 was constructed successfully.
     经转化蓝藻Synechococcussp . PCC794 2细胞 ,在 4 2℃热诱导 30min后 ,目的基因UB Tα1得到较高水平表达 ,表达量约占可溶性蛋白的 8.7%
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  espressed
     Methods The constructed plasmid pPIC9K-IL18 aslinarized with Sac I and transformed into Pichia. pastoris GS115. His+Mut+ transformants was screened outand expressed in BMGY and BMMY media. The espressed products were analyzed by SDS-PAGE andWestern blot .
     方法 用Sac I 将pPIC9K-IL18线性化后,电转化毕氏酵母GS115,筛选后得到His+Mut+转化子,在BMGY和BMMY培养基中进行诱导表达,表达产物进行SDS-PAGE和Western blot 分析。
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  “表达表达”译为未确定词的双语例句
     A total of 19 CYP450 genes expression were detected in NPC a nd non-cancerous nasopharynx tissues, and the expression levels of CYP1B1, CYP2 C8, CYP2C9, CYP2D6, CYP3A5, and CYP4B1 were higher than those of the other genes .
     总共有19个CYP450基因在鼻咽癌及鼻咽非癌组织表达,表达较高的基因有CYP1B1、CYP2C8、CYP2C9、CYP2D6、CYP3A5和CYP4B1。
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     The expression rate of COX 2 mRNA , MMP2 and MMP9 were 62.69 %、 56.70 % and 58.20 %.
     结果  COX- 2 mRNA及 MMP2、MMP9在星形细胞瘤中高度表达,表达阳性率分别为62.69%、56.70%和58.20%;
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     Results: The recombinant expression vectors with two regulatory elements combination(CHEF1-α5′UTR+3′ UTR 1.65 kb,CHEF1-α5′UTR +3′ UTR 2.7 kb) increased the expression efficiency by 65.9% and 54.5% respectively.
     结果:CHEF1-α5′UTR及启动子+3′UTR 1.65 kb、CHEF1-α5′UTR及启动子+3′UTR 2.7 kb这2种组合均可有效提高目的基因tPA的表达,表达效率与对照载体相比分别提高了65.9%和54.5%。
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     Meantime, no expression of E cad protein was observed in 7 of 7 OC with metastasis but in 6 of 13(46.2%)of those without metastasis ( P =0.044).
     无转移卵巢癌原发灶 E- cad蛋白表达阳性率为 46 .2 % (6 / 13) ,而有转移者 7例均无阳性表达 ,表达率为 0 ,P=0 .0 44。
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     The positive rate of Tyr-397 FAK protein expression in the cancer tissues was 90%, while the positive rate in the corresponding normal tissues was only 20% (c2 = 17.1, P <0.01).
     20例结肠癌组织18例有FAKTyr397磷酸化蛋白表达,表达率为90%,而对应癌旁组织仅有4例有FAKTyr397磷酸化蛋白表达,表达率为20%(c2=17.1,P<0.01);
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  相似匹配句对
     Abnormal expression of TGF- ?
     的表达
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     Pretreatment of L-Arg or L-NAME potentiated or reduced formalin-induced increased expression of Fos.
     S的表达
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  level expression
Cloning, high-level expression, and characterization of the somatotropin (ST) gene of an indigenous Nili-Ravi breed of water buffalo Bubalus bubalis (BbST) are described.
      
High-level expression could be obtained by synthesizing a codon-optimized ST gene and expressing it in the form of inclusion bodies.
      
On recent evidence, this LCR is necessary for high-level expression of the globin genes, but not for the active state of the domain.
      
The Context Organization of Functional Regions in Yeast Genes with High-Level Expression
      
Genotype CC, which determines low-level expression of IL-6, occurred at a frequency of 0.35 in patients with low-progressing MM and was absent from patients with aggressive MM.
      
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A cDNA fragment of myosin light chain kinase (MLCK) containing SalⅠsite and ATG was generated by polymerase chain reaction (PCR). The pBKrsv MLCK was constructed by inserting MLCK cDNA into pBKrsv polylinker and identified by restriction mapping sequence analysis of SalⅠ site. This work provides the foundation for further investigation of MLCK exprssion MeSH polymerase chain reaction (PCR); myosin light chain kinase (MLCK); pBKrsv; restriction endonuclease mapping assay; DNA

用聚合酶链反应(polymerasechainreaction,PCR)方法扩增出含有SalⅠ位点和转录起始点ATG的肌球蛋白轻链激酶(myosinLightchainki-nase,MLCK)cDNA片段,并插入质粒pBKrsv中,构建成能在宿主细胞中表达的表达载体,并通过酶切图谱、SalⅠ位点DNA序列分析得到证实。为进一步研究MLCK的表达奠定基础。

In order to study the signficance of laminin(LN), type Ⅳ collagen(Col Ⅳ)and fibronectin(FN) in breast cancer progress, we determined immunohistochemically their expressions in the tissues of 113 primary breast carcinomas and 37 lymph node metastasis by LSAB mothed. The expressions of the three proteins, which looked as a continuous line, was identified on BM in noninvasive breast carcinoma, in which, the integral light density of expression of type Ⅳ collagen and laminin in breast cancer in situ was higher...

In order to study the signficance of laminin(LN), type Ⅳ collagen(Col Ⅳ)and fibronectin(FN) in breast cancer progress, we determined immunohistochemically their expressions in the tissues of 113 primary breast carcinomas and 37 lymph node metastasis by LSAB mothed. The expressions of the three proteins, which looked as a continuous line, was identified on BM in noninvasive breast carcinoma, in which, the integral light density of expression of type Ⅳ collagen and laminin in breast cancer in situ was higher than that in normal breast tissues( P <0 02). The expressions of all the three proteins were found fragmentally around some cell nests in invasive carcinomas, in which, type Ⅳ collagen was associated with laminin ( P <0 05). It was associated with better prognosis ( P <0 01) that FN expression was observed in the stroma of invasive carcinoma. Also, the expression of the three protiens was detected in the cytoplasm of breast cancer cells (19 5% for type Ⅳ collagen, 27 4% for laminin and FN). FN expression rate of breast cancer cells was related to the invasive ductal carcinoma( P <0 02) and bigger tumor side ( P <0 02). The expressions of the three protiens, in which FN expression rate was higher than other two types ( P <0 01), were detected around cancer cell nets and in cytoplasm of carcinoma cells from lymph node metastasis where the FN expression in cytoplasm was associated with that from primary carcinoma ( P <0 02). It was suggested that the BM proteins could be synthesized by cancer cells themselves and the FN expression in stroma was associated with a better prognosis.

用免疫组化法观察了层粘连蛋白(LN)、Ⅳ型胶原(Col)和纤维连结蛋白(FN)在113例浸润性乳腺癌和其中37例淋巴结转移癌组织中的表达。结果表明,这3种蛋白均可在原位癌结构的基底膜处呈连续状表达。其中ColⅣ和LN平均积分光密度均高于它们在正常小叶基底膜的表达(P<0.05)。3种蛋白在浸润性癌巢周均可表达(表达率为LN38.6%,ColⅣ23.9%,FN84.1%),ColⅣ和LN的表达有相关性(P<0.05)。FN在浸润癌间质中表达与患者预后密切相关(P<0.01)。它们在癌细胞内亦可表达(ColⅣ19.47%、LN27.4%、FN27.4%),其中FN与浸润性导管癌和肿瘤体积大小有关。淋巴结转移癌巢旁和细胞内(LN13.5%,ColⅣ8.5%,FN33.3%)也可见到它们表达,FN在转移癌细胞内的表达率明显高于其它2种蛋白(P<0.01),且与原位癌灶细胞内的表达有关(P<0.02)。表明:癌细胞本身有合成基底膜物质的能力;FN在乳腺癌间质中的表达与预后较好有联系。

To discriminate the expression level of the foreign gene in pBV220 vector, a model was established by predicting RNA secondary structure based on helical regions random stacking, calculating the codon adaptation. The model indicated that there existed statistical significance between high(low) expression and the free energy of secondary structure in the regions 30 ̄39 of 5′ end and 30 ̄39 of 3′ end, while the local codon adaptation of 9 bp in the 3′ end also related to the expression level. Guided by this...

To discriminate the expression level of the foreign gene in pBV220 vector, a model was established by predicting RNA secondary structure based on helical regions random stacking, calculating the codon adaptation. The model indicated that there existed statistical significance between high(low) expression and the free energy of secondary structure in the regions 30 ̄39 of 5′ end and 30 ̄39 of 3′ end, while the local codon adaptation of 9 bp in the 3′ end also related to the expression level. Guided by this model, a pair of PCR primers was designed for expressing ricin A chain gene which was amplified and ligated into pBV220 vector. Transforming DH5α with this recombinant plasmid, ricin A chain was high expressed in DH5α transformant with expression level above 20%. The rationalization of the model was verified by another expression system pExSecI.

基于对螺旋区随机堆积的RNA二级结构的预测和密码子偏性计算,建立了pBV220载体中外源基因高效表达的判别模型。该模型认为,外源基因的表达水平,与其5′端3039区域,以及3′端3039区域的二级结构自由能具有显著的统计学意义;其次与3′端9bp的局部密码子偏性相关。据此模型,我们设计了用于克隆表达ricinA链的引物。将PCR扩增的基因克隆入pBV220载体中,在DH5α宿主中获得了高效表达(表达水平在20%以上),而且在pExSecI载体系统中亦获得了高效表达。

 
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