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蛋白
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  protein
    Protective Role of Ginkgolide B and Praeruptorin on Atherogenesis and Screening Scavenger Receptor Cytoplasmic Domain Binding Protein Using Yeast Two Hybrid System
    银杏内酯B和前胡丙素对动脉粥样硬化的防护机制及酵母双杂交实验体系筛选清道夫受体胞浆域结合蛋白
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    The Experimental Study on Jianpi and Shugan Prescription Regulating Th Cells Differentiation and Expression of Signaling Protein CaN
    健脾、疏肝方药对Th细胞分化及其信号蛋白钙调神经磷酸酶转录表达的影响
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    Effects of RuHuang Pill on Rat Astroglia Structure and Protein Expressions with Hepatic Encephalopathy of Type A
    乳黄片对A型肝性脑病大鼠星形胶质细胞结构与蛋白的影响
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    Effect of Qiangshengubenkeli on CGRP and Mrna Protein Expression of Nos Isoform in Corpus Cavernous Body of Castrated Rats
    中药“强肾固本颗粒”对ED大鼠海绵体勃起神经递质CGRP及NOS亚型mRNA蛋白表达水平影响的实验研究
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    Dynamic Changes of Smad Protein in the Development of Experimental Liver Fibrosis in Rat and Effects of GanxianrongGranule on Liver Fibrosis
    Smad蛋白在实验性肝纤维化过程中的动态变化及肝纤溶颗粒的干预作用
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  proteinum
    Intervention Effect of Puerarin on Expressions of HSP70 and Fas Proteinum in Rats with Acute Cerebral Ischemia Injury
    葛根素对急性脑缺血模型大鼠脑细胞损伤后HSP70及Fas蛋白表达的干预作用研究
短句来源
    We also examined the expression of BGP and BMP-2 gene with the method of RT-PCR and the proteinum expression with the method of WESTERN-BLOT in the bone tissue of osteoporosis rats due to Kidney-Deficiency.
    以RT-PCR法检测大鼠右侧股骨骨钙素BGP基因和骨形成蛋白BMP-2基因表达;
短句来源
    3. apoptosis: compared with Blank group, area density of bcl-2 proteinum descended, area density of bax proteinum ascended(P<0.01) .
    3.细胞凋亡:模型组与空白组比较bcl-2蛋白面密度值降低,bax蛋白面密度值升高(p均<0.01);
短句来源
    Treat group and prevend group compared with model group, area density of bcl-2 proteinum ascended, area density of bax proteinum descended (P<0.01)4. morphology change: sum of neuron of model group was less than that of blank group.
    治疗组、预防组与模型组比较bcl-2蛋白面密度值升高,bax蛋白面密度值下降(p均<0.01)。
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    OBJECTIVE:To study the intervention mechanism of puerarin on expressions of HSP70 and Fas proteinum in rats with acute cerebral ischemia injury.
    目的:探讨葛根素对急性脑缺血模型大鼠脑细胞损伤后热休克蛋白70(HSP70)及Fas蛋白表达的干预作用机制。
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    Therapeutical Effect and Mechanism of Momordicin on BALB/C CVB3 Myocarditis
    苦瓜蛋白对BALB/C小鼠CVB3心肌炎的治疗作用及其机制研究
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    Relationship between Effects of Glucosides of Chaenomeles Speciosa on Collagen-induced Arthritis in Rats and G Proteincoupled Signal Transduction Pathway of Synoviocytes
    木瓜苷对大鼠胶原性关节炎的作用及其与滑膜细胞G蛋白偶联信号转导的关系
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    Biochemical Characteristics and Physiological Functions of Earthworm Fibrinolytic Isozyme III-1 & II from Eisenia Fetida
    蚯蚓蛋白同功酶III-1、II结构与功能的研究
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    DETERMINATION OF SOME ANDROGENS AND ANABOLIC STEROIDS IN HUMAN URINE BY HPLC
    人尿中几种雄激素及蛋白同化激素的HPLC测定
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    Study of the Binding of Glycyrrhizin to Albumin
    甘草甜素与蛋白结合的研究
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  protein
Polyamines May Modulate Both G Protein-Coupled Receptors and G Proteins
      
Heterodimerization of G-Protein-Coupled Receptors Reveals an Unexpected Level of Pharmacological Diversity
      
The Activation Mechanism of Class-III G-Protein Coupled Receptors
      
Hepatic glutathione, lipid peroxides, glutathione peroxidase, alcohol dehydrogenase, aldehyde dehydrogenase, glycogen and total protein in liver were also significantly altered.
      
3D QSAR STUDIES OF INHIBITORS OF CHOLESTEROL ESTER TRANSFER PROTEIN (CETP) BY CoMFA, CoMSIA AND GFA METHODOLOGIES
      
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The "Compound Mixture of Two Fairies" (Ⅰ) has been used as a hypotensive drug. One of its chief ingredients is Phellodendron chinense (Ⅱ). Berberis spp. (Ⅲ)contains also much berbcrine. Hence Ⅱhas been substituted by Ⅲ in making the Compound Mixture Ⅳ. In this paper Ⅱand Ⅲ were studied in the form of fluidextracts. The dosages used were expressed in terms of crude drugs. The intraperitoneal LD_(50) in mice were 2.7 g/kg for Ⅱ and 3.1 g/kg for Ⅲ. Intraperitoneal injections in anesthetized cats produced, in...

The "Compound Mixture of Two Fairies" (Ⅰ) has been used as a hypotensive drug. One of its chief ingredients is Phellodendron chinense (Ⅱ). Berberis spp. (Ⅲ)contains also much berbcrine. Hence Ⅱhas been substituted by Ⅲ in making the Compound Mixture Ⅳ. In this paper Ⅱand Ⅲ were studied in the form of fluidextracts. The dosages used were expressed in terms of crude drugs. The intraperitoneal LD_(50) in mice were 2.7 g/kg for Ⅱ and 3.1 g/kg for Ⅲ. Intraperitoneal injections in anesthetized cats produced, in 90 minutes, an average “% of hypotensive areas”of -30%for Ⅰ(4g/kg),—30% forⅡ(2g/kg),—42% for Ⅲ(2g/kg), and —32% for Ⅳ(4g/kg). Six renal hypertensive dogs were fed Ⅳ 15—30 g/kg/day for 20 days. There were no remarkable changes in blood pressure, EKG, BSP retention, and blood NPN. Three normal dogs were fed daily Ⅳ 45 g/kg for 3 weeks. Abnormal alteration was not found in EKG, liver and kidney function tests, nor was toxic sign noticed in the microscopic examinations. Sixteen water-soluble portions from different fractions of Ⅲ were screened over by single intravenous injections in anesthetized cats, 2—3 cats for each portion. Six of them yielded an average“%of hypotensive areas” exceeding —30%. Since Ⅲ is abundant in China and low in toxicity, it seems to be a promising source of hypotensive drugs.

复方二仙合剂降压有效,其中以黄蘗的降压作用較強。三顆針是小蘗科植物,与黄蘗同为小蘗碱的資源植物,来源充沛。本文試驗其毒性与疗效。小白鼠腹腔注射1次三顆針或黃蘗流浸膏,观察3天的LD_(50)各为3.1与2.7克生药/公斤,相差不显著。麻醉猫腹腔注射三顆針或黄蘗流浸膏2克/公斤,90分钟內平均降压面积百分比:三顆針組为-42%,黃蘗組为-30%,相差显著。而注射針仙合剂(三顆針代黄蘗加入二仙合剂)或二仙合剂4克/公斤,90分钟內的平均降压面积百分比均为-30%左右。 腎型高血压狗每天灌服針仙合剂15—30克/公斤,共20天,血压变化不明显。心电图、肝对磺溴酞鈉的排泄机能及全血非蛋白氮量的測定,无明显改变。正常狗每天灌服針仙合剂45克/公斤,3周后,检查心电图、肝及腎机能无异常,其中1狗內脏作組織切片,无明显中毒現象。对麻醉猫靜脉注射由三顆針提取的不同部位的水溶液2—10毫克/公斤,篩过16种成分,其中6种的平均降压面积百分比超过-30%。三顆針药源广、口服毒性小,是一种較有希望的降压药源。

Thirteen vegetable drugs used in Chinese traditional medicine or folk medicine were screened for antiphlogistic activity in animals. Irritation arthritis produced in rats by subcutaneous injections of either formalin or egg white to the ankle joint of the hind leg was employed for the test. The decoctions and (or) alcoholic extracts made from the drugs were administered orally at dosages corresponding to 10 gm crude drug per kg 30—40 minutes prior to the injection of egg white or 5 gm (crude drug)/kg once daily...

Thirteen vegetable drugs used in Chinese traditional medicine or folk medicine were screened for antiphlogistic activity in animals. Irritation arthritis produced in rats by subcutaneous injections of either formalin or egg white to the ankle joint of the hind leg was employed for the test. The decoctions and (or) alcoholic extracts made from the drugs were administered orally at dosages corresponding to 10 gm crude drug per kg 30—40 minutes prior to the injection of egg white or 5 gm (crude drug)/kg once daily for 5 days starting 24 hours after the injection of formalin. The results indicate that pretreatment of the animals with preparations of Pottsia laxiflora O. Ktze, Elephantopus tomentosus L., Zingiber officinale Rose, and Serissa sp. markedly inhibited the development of egg white irritation arthritis. Ledebouriella seseloides Wolff and Asarum sieboldi Miq. were less active. Although the development of egg white irritation arthritis was not inhibited by Coix lackryma-jobi L., Dipsucus sp., Achyranthes bidentata Bl. (alcoholic extracts), Eupatorium stoeckadosmum Hance (alcoholic extract), and Elephantopus scaber L., the swelling of the ankle joints regressed more rapidly under the influence of these preparations. Of the 6 preparations tested for the treatment of formalin arthritis, alcoholic extracts of A. bidentata and E. scaber were shown to be more effective than A. sieboldi, C. lackryma-jobi and Serissa sp., while L. seseloides was inactive.

用大鼠蛋白性及甲醛性关节炎的病理模型,对十三种中医临床已用或祖国医学文献上记载的中药和从地方搜集的民间单方进行了抗关节炎作用的研究.发现对大鼠蛋白性关节炎的抑制作用,以腰骨藤茎叶及白花地胆草为最突出,生姜、六月雪及腰骨藤根等次之,防风及细辛等则稍弱.对大鼠甲醛性关节炎的治疗作用则以怀牛膝酒剂及地胆草酒剂为最明显,细辛、薏仁及六月雪亦有一定作用.

Forty-nine adult male albino rats, weighing between 140--210 gms were selectedfor study. Among them, 9 served as controls and the rest were injected daily with 25 mgof cortisone or hydrocortisone into the gluteal muscles and they were sacrificed after 1,2, 3, and 6 injections and 4, 5, and 9 days after the 6th injection. Liver blocks fromboth experimental and control groups were taken immediately after death and were pro-cessed with routine histological and histochemical techniques. After the administration...

Forty-nine adult male albino rats, weighing between 140--210 gms were selectedfor study. Among them, 9 served as controls and the rest were injected daily with 25 mgof cortisone or hydrocortisone into the gluteal muscles and they were sacrificed after 1,2, 3, and 6 injections and 4, 5, and 9 days after the 6th injection. Liver blocks fromboth experimental and control groups were taken immediately after death and were pro-cessed with routine histological and histochemical techniques. After the administration of cortisone, the eosinophilic granules, protein-bound tyro-sine, histidine and tryptophan, sulfhydryl and disulfide groups, and ribonucleic acid con-spicuously decreased in amount in liver cells. Hence these cells were filled with clearvacuoles of various sizes in the cytoplasm and assumed the name of "balloon cells." Cortisone caused an immediate rise of liver glycogen and phosphorylase activity. The activity of acid and alkaline phosphatases increased in the liver tissues in thecarly stages of treatment and the change of the latter enzyme was more apparen.Later, it was reduced and did not recover to the normal level even on the 9th day afterthe last injection. The activity of adenosine triphosphatase and 5-nucleotidase, elevatedat the beginning of the treatment, returned to normal afterwards. The activity of nonspecific phosphatase in the liver tissues of all the animals of theexperimental group was kept at a lower level than in the normal controls. Nonspecificesterase reacted in the same but more exaggerated manner. Cytochrome oxidase activity in the liver cells apparently decreased after the adminis-tration of cortisone, but it gradually recovered after the injection was suspended. The influence of cortisone and hydrocortisone upon glycogen, ribonucleic acid, andvarious enzymes was the same, but they showed different actions on proteins and pro-tein-bound sulfhydyl and disulfide groups. Maximum depletion of the latter substanceswas attained after a single injection of cortisone, it required 6 injections of hydrocor-tisone to get a similar effect apparently the action of the former compound is more im-mediate and more pronounced than that of the latter.

1.实验用49只成年雄性大白鼠,体重为140—210克。其中9只作为对照。实验动物分别经臀部肌肉注射可的松或氢化可的松25毫克/每日一次。分注射一次、三次、六次以及注射六次后恢复4、5和9日四组。取材后,按组织学及组织化学常规处理,进行观察。 2.注射可的松后,肝细胞中嗜伊红性颗粒和含酪氨酸、组氨酸及色氨酸的蛋白貭、结合-SH和结合-S-S-化合物以及RNA的含量均显著减少。因此胞貭中出现大小不等的明亮空泡,或称之为气球样细胞。 3.可的松促使肝糖原急剧增加,与其相伴磷酸化酶的活性也增强。 4.ACP和ALP的活性在注射初期增强,ALP更加明显。之后酶的活性下降,到注射停止后9日尚未完全恢复。ATP酶和5-核甙酸酶的活性在注射初期略有升高,以后则逐渐恢复正常。非特异性磷酸酶的活性,在实验动物中,始终低于正常。而酯酶活性则显著下降,注射停止后9日,仍未恢复正常。注射后肝细胞中细胞色素氧化酶的活性显著降低,注射停止后则逐渐恢复。 5.实验结果表明可的松和氢化可的松对肝糖原和RNA的含量以及对各种酶的影响是一致地。然而对蛋白貭、结合-SH和结合-S-S-化合物含量的影响却不完全相同。注射可的...

1.实验用49只成年雄性大白鼠,体重为140—210克。其中9只作为对照。实验动物分别经臀部肌肉注射可的松或氢化可的松25毫克/每日一次。分注射一次、三次、六次以及注射六次后恢复4、5和9日四组。取材后,按组织学及组织化学常规处理,进行观察。 2.注射可的松后,肝细胞中嗜伊红性颗粒和含酪氨酸、组氨酸及色氨酸的蛋白貭、结合-SH和结合-S-S-化合物以及RNA的含量均显著减少。因此胞貭中出现大小不等的明亮空泡,或称之为气球样细胞。 3.可的松促使肝糖原急剧增加,与其相伴磷酸化酶的活性也增强。 4.ACP和ALP的活性在注射初期增强,ALP更加明显。之后酶的活性下降,到注射停止后9日尚未完全恢复。ATP酶和5-核甙酸酶的活性在注射初期略有升高,以后则逐渐恢复正常。非特异性磷酸酶的活性,在实验动物中,始终低于正常。而酯酶活性则显著下降,注射停止后9日,仍未恢复正常。注射后肝细胞中细胞色素氧化酶的活性显著降低,注射停止后则逐渐恢复。 5.实验结果表明可的松和氢化可的松对肝糖原和RNA的含量以及对各种酶的影响是一致地。然而对蛋白貭、结合-SH和结合-S-S-化合物含量的影响却不完全相同。注射可的松一次后上述成分减少的最明显,而氢化可的松,则以注射六次后改变的最显著,就是说,前者较后者对蛋白貭、结合-SH和-S-S-化合物的作用迅速而强烈。

 
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