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蛋白
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  protein
    Analysis and Microsequence of Human Erythrocyte Pyrimidine5'-Nucleotidase Protein and Construction of cDNA Library
    人红细胞嘧啶5’-核苷酸酶蛋白的分析鉴定、微量测序及其cDNA文库的构建
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    Investigation of Production and Immuno1-Protection of Plasmodium falciparum Major Merozoite Surface Protein 1
    恶性疟原虫主要裂殖子表面蛋白1的表达及免疫保护作用的研究
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    Construction and eukaryotic expression of anti-LPS Fab,BPI and Fab-BPI fusion protein
    抗LPS Fab、BPI和Fab-BPI融合蛋白真核表达载体的构建及其在CHO细胞中的表达
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    Cloning of the Gene of Protein Interacting with HCV Polyprotein and Functional Study of Hcbp6
    丙型肝炎病毒蛋白相互作用蛋白基因克隆化及Hcbp6功能研究
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    The Structure and Function of E.coli Era and Its Interactional Protein
    E.coli Era及其相互作用蛋白的结构与功能研究
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  egg albumen
    The myelin basic protein and egg albumen were (all for Sigma).
    髓鞘碱性蛋白、卵白蛋白(均为Sigma)。
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    ② The tiltboard experiment angle in rats in immunity group: Twenty-eight days after spinal cord injury, it was significantly bigger in myelin basic protein group and complete spinal cord homogenate immunity group than that in egg albumen group (65°, 60° vs 35°, P <0.01).
    ②免疫组大鼠斜板实验角度:脊髓损伤后28d,髓鞘碱性蛋白和全脊髓匀浆免疫组均显著大于卵白蛋白组(65°,60°比35°,P<0.01)。
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  “蛋白”译为未确定词的双语例句
    The preparation of the monoclonal antibodis against polycystin-1 and their application in the study of pathogenesis of ADPKD
    抗多囊蛋白-1单克隆抗体的制备及在ADPKD发病机理研究中的应用
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    Experimental Study on the effects of Osteoblast Differentiation and Quantitative Bioassay of rhBMP
    重组人骨形成蛋白成骨分化及其定量测活的研究
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    Expression of the Bioactive NS5 of Dengue Virus in Prokaryocytes and Screening Its Antagonistic Peptide
    登革病毒NS5蛋白在原核细胞中的活性表达及其拮抗肽筛选
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    The Molecular Basis of Neuroglobin and Its Significance in Protecting Brain from Hypoxic Injure
    脑红蛋白(NGB)作用的分子基础及其在脑缺氧损伤防护中的意义
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    Mechanism for Regulation of HLA-I Expression on HepG2 Cells with HBV Wild Type and Nucleocapsid Mutants
    HBV野生株和核壳蛋白变异株在HepG2细胞的HLA-I表达及调节机制
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  protein
Polyamines May Modulate Both G Protein-Coupled Receptors and G Proteins
      
Heterodimerization of G-Protein-Coupled Receptors Reveals an Unexpected Level of Pharmacological Diversity
      
The Activation Mechanism of Class-III G-Protein Coupled Receptors
      
Hepatic glutathione, lipid peroxides, glutathione peroxidase, alcohol dehydrogenase, aldehyde dehydrogenase, glycogen and total protein in liver were also significantly altered.
      
3D QSAR STUDIES OF INHIBITORS OF CHOLESTEROL ESTER TRANSFER PROTEIN (CETP) BY CoMFA, CoMSIA AND GFA METHODOLOGIES
      
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  egg albumen
We analysed CBP in an eider population in the central Baltic Sea 2001-2002, using non-destructive egg albumen sampling combined with protein fingerprinting.
      
Five groups of individually housed albino rats (n=7, initial average weight=48 g) were fed diets based on egg albumen and cornstarch (basal diet 8.2 g Ca, 6.0 g P, 0.7 g Mg, 225 mg Zn, 150 mg Fe, 60 mg Mn, 8 mg Cu, and 5 mg Cd) over a 4-wk period.
      
The activator failed to attack casein, pure fibrin, gelatin and egg albumen.
      
The total 14C radioactivity in egg albumen was 7.29% of the administered dose as compared to 4.67% in egg yolk.
      
Active sensitization was performed by subcutaneous injection of egg albumen.
      
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~~

大白鼠接受1100倫琴X綫全身照射。照射後1—8晝夜時靜脈內注入標記(I~(131)及S~(35)血漿蛋白。注入後5、15、30分鐘及30分鐘後到360分鐘每隔半小時取血,2或6小時後殺死動物取其內臟,製備血液及組織標本,测其放射性。藉以觀察標記血漿白血液排出之速度及其在體內各器官中之分佈。實驗結果發現,照射後標記血漿蛋白自循環血液中排出加速,这一加速尤以4—6晝夜時为著。此時,標記血漿蛋白注入後30—120分鐘间,對照鼠的半排出時達照射鼠的1.44—2.7倍。照射後標記血漿蛋白在各器官中之含量亦見增多,這一增多尤以肺、腸、心、胃等器官为主。作者認为這些变化表明,致死量X綫全身照射引起了大白鼠血管對標記血漿蛋白通透性的增高。

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi...

The cells of the apical ectodermal thickening, neighboring ectoderm and underlyingmesenchyme of the limb buds of mouse embryos, 11--13 days vaginal plug age, wereused for this study. Most of the specimens were fixed in 1% osmic acid in veronalacetate buffer, pH 7.6, 4℃, 1 hour and embedded in methylchrylate. Parts of the speci-mens were either fixed in potassium permagnate or stained in lead hydroxide after osmicacid fixation. Observations and photographs were made under SEM Ⅲ electron micros-cope. The Golgi apparatus appeared to be horse-shoe shaped in the apical ectodermalthickening and S-shaped in the neighboring ectoderm. It consisted of small vesicles andtubules without any large vesicle. The mitochondria in the neighboring ectoderm were usually round or oval in shape,with fewer and irregular cristae and lighter matrix. The mitochondria of the mesenchy-mal cells were even more embryonic in structure with only 2--3 oblique cristae and alarge inner chamber. The mitochondria in the supperficial layers of the apical ectoder-mal thickening were more or less similar to those in the neighboring ectoderm. Rod-shaped and filamentous mitochondria increased in number in the deeper layers of theapical thickening. Such filamentous mitochondria had vertical cristae, denser matrix andoval and light inner chambers. Transitional stages between the very small vesicles withvague cristae and the large mitochondria with distinct cristae were found in our leadstained preparations. Except in the Golgi region, ribosomes grouped into ring or tubular forms weredistributed all over the cytoplasm in the three kinds of the embryonic cells studied.Transitional stages between such ribosome clusters and the granulated vesicles and tubulescould be clearly demonstrated in the lead stained specimens. In the apical ectodermal thickening, granulated endoplasmic reticulum in the formof scattered small vesicles and tubules seemed to be more abundant than in the neighbor-ing ectoderm and underlying mesenchymal cells. But the most striking difference foundin the apical thickening was the expansion of the granulated endoplasmic reticula intolarge saccules, the cisternae of which containing a grayish dense substance. Another interesting phenomenon found in the apical ectodermal thickening was thepresence of dense bodies in the cytoplasm. They were in various sizes and densitieswith dense granules, masses or cords and various forms of vesicles. They might be round,oval or irregular with a complete, partial or entire absence of limiting membrane. Thosewithout membrane were usually irregular in form and could hardly be demarkated from their surrounding cytoplasm whence assembly of ribosome clusters, mitochondria andendoplasmic reticulum from the surrounding cytoplasm to form the dense bodies could befound. Preliminary histochemical studies found them to be positive in alkaline and acid-phosphatases and RNA staining. They were therefore considered to be lysosomes orcytosomes. The significance of the differences in mitochondrial form and structure, abundantand expanded cisternae of the granulated endoplasmic reticulum and the presence of thedense bodies in the apical ectodermal thickening in relation to embryonic differentiationof the limb was discussed.

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的...

用小白鼠胚胎阴栓日龄11—13天的前肢芽和后肢芽,锇酸固定,甲基丙烯酸甲酯及甲基丙烯酸丁酯包埋,在SEM Ⅲ型电子显微镜观察了肢芽尖端增厚外胚层、邻近普通外胚层及间充质细胞。部分材料用过锰酸钾固定或锇酸固定后氢氧化铅染色。各种细胞相互比较的结果如下: 1.两种外胚层的高尔基体极相似,均由小泡及小管组成,无大泡,横切时成为成群的小泡。普通外胚层细胞的高尔基体呈S形,增厚外胚层的马蹄形。 2.线粒体在普通外胚层卵圆形或圆形,嵴不整齐。增厚外胚层的线粒体在浅层细胞少,结构与普通外胚层相似,但愈至深层则细长的线粒体愈多。细长线粒体的嵴较密,亦较整齐,与表面垂直,基貭亦较致密。在尖端增厚外层细胞见到由胞质新形成的小泡过渡到小圆的线粒体。 3.无论在普通外胚层下或在尖端增厚外胚层下的间充质细胞,其线粒体的形状和结构相同,都是圆或卵圆,嵴少而靠近表面作半月状,内室大,基貭密度低,属比较原始的胚胎型。 4.嗜碱质在两种外胚层及间充貭细胞均以弥散的核朊粒为主,由5—10余粒组成小群落。这些群落在尖端增厚外胚层较大、较密,粒亦较多。普通外胚层细胞的有粒内貭网或动貭较间充貭为多,二者均系胚胎型,是分散的小泡或小管。尖端增厚外胚层的动貭有独特形态,即池的一端扩张成大泡,内含网状致密物,显然含有较多的,要不是特异的蛋白貭。这种扩张动貭对于胚胎分化及诱导的关系曾加讨论。 5.动质的形成有证据表明是先从核朊粒群落出现膜成为动貭小泡或小管,再并合成较大的小泡和较长的小管。 6.增厚外胚层细胞的另一特点是比较普逼的存在着形状、大小及密度不同的致密体。有的完全致密,有的泡状,有的是二者的混合体,有的界限清楚,有的处于分散状态。在形态上类似溶酶体和卵子的多泡体。内含类似核朊粒的致密粒、退化线粒体及动质膜。这些致密体和细胞膜、核膜及高尔基体未显有何关系。基膜及细胞膜完好。未见增厚外胚层细胞有排出、吞食或饮液现象,亦未见致密体排至中胚层,故认为这些致密体起于胞貭。对于分化诱导关系曾提出讨论。 7.细胞核及核膜在三种细胞未见有显著不同。核膜有孔,其外膜可与动貭膜相连,核仁小,紧靠核膜,未见有排出现象。氢氧化铅染色的标本有时显示染色貭有微丝,这在胞貭核朊粒群落亦可出现。 8.细胞膜完整,比较直,膜的内侧有一层致密物貭,无桥粒。

It has been already established that the person with erythrocyte glucose-6-phosphate dehydrogenase deficiency is liable to develop favism. It is generally accepted that the heterozygote does not develop the disease. In order to detect heterozy-got.es, the writer has used three methods (namely MR-microhistochemical elution test, MR-mierophotometric test, and glutathione stability test), and found that the MR-microhistochemical elution test is most sensitive, detecting heterozygotes in 92%. Moreover, there are...

It has been already established that the person with erythrocyte glucose-6-phosphate dehydrogenase deficiency is liable to develop favism. It is generally accepted that the heterozygote does not develop the disease. In order to detect heterozy-got.es, the writer has used three methods (namely MR-microhistochemical elution test, MR-mierophotometric test, and glutathione stability test), and found that the MR-microhistochemical elution test is most sensitive, detecting heterozygotes in 92%. Moreover, there are 17 females with history of favism 10 out of 17 females with history of favism are found to be heterozygotes. This indicates clearly that heterozygotes are liable to suffer the disease after ingestion of fava beans.In addition, according to the degree of expressivity shown by the microhistoche-mical elution method, the heterozygotes can be devided into three types: the reactive, the intermediate, and the non-reactive. Out of 44 cases of heterozygote, there are 29 cases of reactive type (65.9%), evidently being more than the other two types. Among 11 cases of heterozygotes with history of favism, there are 10 reactive including the intermediate. This illustrates that the occurrence of favism is more or less related to the degree of expressivity. Therefore, the reactive and intermediate types should be included as an object of prophylactic measures.

蚕豆病是进食蚕豆后引起的一种急性溶血性疾病。己经查明,红细胞中缺乏6-磷酸葡萄糖脱氢酶是对蚕豆敏感的主要原因。这种酶缺乏是按伴性不完全显性规律遗传的。一般认为,携带此病理基因的杂合子不发病。本文探讨鉴定杂合子较敏感的方法,并研究杂合子是否发病。在比较了高铁血红蛋白还原试验微量洗脱法、微量比色法和谷胱甘肽稳定性试验后认为,前法较后二法敏感。 用上述三种方法检查了17例有蚕豆病史的女性,证明其中10例为杂合子,6例为纯合子。说明杂合子可以发病。若将杂合子按微量洗脱法显示的表现度分为三型:即反应型、中间型和非反应型,则有患病史的杂合子绝大多数属于反应型及中间型(11例中之10例)。说明杂合子是否发病与其表现度有关。故建议在预防工作中,将此二型列入预防对象。

 
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