助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   western印迹 的翻译结果: 查询用时:0.076秒
图标索引 在分类学科中查询
所有学科
生物学
基础医学
泌尿科学
肿瘤学
植物保护
消化系统疾病
临床医学
心血管系统疾病
妇产科学
更多类别查询

图标索引 历史查询
 

western印迹     
相关语句
  western blot
     10μmol/L and 20μmol/L lactacystin were chosen for treating SH-SY5Y cells,and the cellular polyubiquitinated proteins were detected by Western blot.
     选取10μmol/L和20μmol/L lactacystin处理SH-SY5Y细胞,Western印迹法检测细胞内多泛素化蛋白的含量。
短句来源
     Western blot method was used to detect the effects of TGF-β 1 on the expression of ERK 1 /ERK 2proteins.
     Western印迹法观察不同剂量TGF β1对ERK1/ERK2 蛋白表达的影响。
短句来源
     The mRNA and protein expressions of h/mTIMP-1, mTIMP-1, TIMP-2, TIMP-3, MMP-9, MMP-2, and COLⅣα5 mRNA were detected by Northern blot and Western blot.
     采用Northern杂交、Western印迹方法检测两组小鼠肾组织内h/mTIMP-1、mTIMP-1、TIMP-2、TIMP-3、MMP-9、MMP-2和COLⅣα5mRNA及蛋白质表达;
短句来源
     The expression of Kv1.3 and Kir2.1 channels were examined by immunocytochemistry, RT-PCR and Western blot.
     采用免疫细胞化学、RT-PCR和Western印迹检测人单核细胞源性巨噬细胞和泡沫细胞上Kv1.3和Kir2.1的表达。
短句来源
     RT-PCR, Western blot method were used to examine the expression changes of ICAM-1 and VCAM-1 after 12 weeks.
     用RT-PCR及Western印迹的方法检测12周后ICAM-1、VCAM-1表达的改变。
短句来源
更多       
  western blotting
     Establishment of IgE mediated Western blotting in identifying IgE BF/sCD23
     鉴定IgE BF/sCD23的IgE介导的间接Western印迹技术的建立
短句来源
     The expressions of Fas,FasL, Bcl-2,Bax,caspase-3,caspase-8, cytochrome c and phosphorylations of JNK,p38 and extracellular signal-regulated kinase(ERK)1/2 were analysed by Western blotting.
     Fas,FasL,Bcl-2,Bax,caspase-3,caspase-8,细胞色素c的表达以及JNK,p38,细胞外信号调节激酶(ERK)1/2的磷酸化水平用Western印迹检测。
短句来源
     Methods 6A8α-manosidase expression was detected by Western blotting.
     方法Western印迹检测6A8α-甘露糖苷酶的表达。
短句来源
     Methods HIT-T15 cells were incubated with palmitate(0, 0.25, 0.5, 1.0mmol/L) (presence of 11.1mmol/L glucose) for 24 hours before the cells were stimulated with 100nmol/L insulin for 5 min,Western blotting was used to assess the levels of PKB threonine phosphorylation.
     方法 HIT-T15细胞分别与0,0.25,0.5,1.0mmol/L的软脂酸(同时存在11.1mmol/L葡萄糖)孵育24h后,以100nmol/L的胰岛素急性刺激5min,用Western印迹法检测PKB苏氨酸磷酸化水平。
短句来源
     Western blotting showed that differentiation resulted in a 2.08-fold,3.15-fold(P<0.05) and 1.24fold(P>0.05) increment in the expression of SR-BI protein compared with U937 monocytes.
     W estern印迹法中诱导分化组SR-B I蛋白表达量分别是未分化组的2.08(P<0.05)、3.15(P<0.05)和1.24倍(P>0.05)。
短句来源
更多       
  by western blot
     10μmol/L and 20μmol/L lactacystin were chosen for treating SH-SY5Y cells,and the cellular polyubiquitinated proteins were detected by Western blot.
     选取10μmol/L和20μmol/L lactacystin处理SH-SY5Y细胞,Western印迹法检测细胞内多泛素化蛋白的含量。
短句来源
     The expression of Smad7,α-SMA, E-cadherin and ColⅠprotein was detected by western blot.
     Western印迹方法检测不同时间点Smad7、α-SMA、E-钙黏蛋白(cadherin)和ColⅠ蛋白的表达水平。
短句来源
     10μmol/L and 20μmol/L lactacystin were also chosen for treating SH-SY5Y cells transfected transiently by parkin gene,then the cellular viability was evaluated by the mono-nuclear cell direct cytotoxicity(MTT)assay,the cellular polyubiquitinated proteins were detected by Western blot,and the cellular ubiquitin-positive inclusions were detected by immunofluorescence.
     瞬时转染parkin至SH-SY5Y细胞,并以MTT法检测10μmol/L和20μmol/L lactacystin作用下的细胞活力,Western印迹法检测细胞内多泛素化蛋白的含量,以及用免疫荧光法检测泛素阳性包涵体的形成。
短句来源
     Active caspase 3 and bcl-2 degradation were assessed by Western blot.
     以Western印迹检测caspase 3的活化以及bcl-2和bcl-2的降解产物。
短句来源
     Expression of a-SMA, E-cadherin and collagen I proteins was detected by Western blot.
     Western印迹检测α-SMA、 E-cadherin和Col I蛋白的表达。
短句来源
更多       
  western blot assay
     Results The proteins purified in culture supernatant was confirmed to be CD28-Ig fusion protein by RT-PCR, SDS-PAGE and Western blot assay.
     结果经RT-PCR,SDS-PAGE及Western印迹鉴定,CHO细胞上清中纯化的蛋白是CD28与Ⅰg的融合蛋白,并获得纯度较高的融合蛋白。
短句来源
     After the induction by 2% methanol for 48 hours, the expression of dimeric hTFF3 came up to 45% of total proteins in medium, as identified by SDS PAGE and Western blot assay.
     2 %甲醇诱导酵母表达 48h后 ,上清经SDS PAGE和Western印迹证明重组蛋白质以双体的形式分泌表达 ,占总蛋白质的 45 % ,能被抗hTFF3抗体所识别。
短句来源
     Quantitative reverse transcription PCR(RT-PCR) was used to detect the mRNAs of Smad3 and Smad4. The expressions of Smad3 and Smad4 protein were detected by Western blot assay.
     RTPCR检测LNCaP细胞中Smad3、Smad4mRNA水平,Western印迹法检测Smad3、Smad4蛋白表达情况。
短句来源
     Western blot assay was adopted to examine the expression level of survivin.
     通过Western印迹法检测Survivin表达量。
短句来源
     Western blot assay confirmed that the expression of caveolin-1 in pulmonary squamous cell carcinoma and adenocarcinoma was lower than in surrounding non-neoplastic lung tissues (P<0.01).
     Western印迹结果进一步证实caveolin-1在肺鳞癌、肺腺癌组织中的表达均显著低于癌旁正常肺组织,P<0.01。
短句来源
更多       

 

查询“western印迹”译词为其他词的双语例句

 

查询“western印迹”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  western blot
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis revealed that T800/T1300 were highly expressed in the form of an inclusion body induced by isopropylthiogalactoside.
      
Western blot showed that the xooR gene was successfully expressed in vitro in E.coli as a XooR-GFP fusion protein with a size of 54 ku.
      
harveyi TS-628 strain isolated from infected groupers were extracted and Western blot analysis was used to detect the antigenicity of these extractions.
      
Results of the Western blot assay reveal that there are four positive flagellin bands: 35 kDa, 38 kDa, 43 kDa, and 52 kDa, of which the 43 kDa and 52 kDa bands displayed the strongest positive reaction.
      
HIF-1α protein was measured by Western blot method.
      
更多          
  western blotting
Western blotting analysis showed that TM3 protein was purified with affinity purification.
      
The recombinant virus was confirmed using PCR, Southern blotting and Western blotting.
      
The cultural supernatant was collected and tested by SDS-PAGE and Western blotting.
      
Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity.
      
Western blotting demonstrated that the pool of 26S proteasomes in ascitic carcinoma Krebs-II was twice that in control lung cells and did not significantly differ by total 26S proteasome quantities from the spleen and liver.
      
更多          
  by western blot
HIF-1α protein was measured by Western blot method.
      
The expression of JWA protein was determined by Western blot analysis.
      
The influence of inhibition of cubilin on albumin-induced expressions of monocyte chemoattractant protein 1 (MCP-1) and regulated upon activation normal T-cell expressed and secreted (RANTES) was investigated by Western blot.
      
The cDNA fragment was then cloned in a bacterial expression vector and expressed in Escherichia coli as a ~57 kD fused protein, and its presence was further confirmed by Western blot analysis.
      
Mitochondrial porin was identified in Rickettsia prowazekii by Western blot analysis of whole cells and membrane fractions with monoclonal antibody against porin VDAC 1 of animal mitochondria.
      
更多          
  western blot assay
Results of the Western blot assay reveal that there are four positive flagellin bands: 35 kDa, 38 kDa, 43 kDa, and 52 kDa, of which the 43 kDa and 52 kDa bands displayed the strongest positive reaction.
      
SDS-PAGE and Western blot assay indicated that fusion protein GST-IE86 with molecular weight of 92 ku is soluble in the supernatant of cell lysate.
      
A recombinant virus expressing HIV-1 protein MEGp24 was screened by genome PCR and Western blot assay.
      
Western blot assay showed that z-ajoene Inhibited proto-oncogene bcl-2 expression in the three different kinds of tumor cells, suggesting that z-ajoene may be a potential agent for tumor chemotherapy.
      
RT-PCR was used to determine the mRNA of NF-κB in the cells, Western Blot Assay was used to determine the protein content of NF-κB in the cells.
      
更多          
  其他


Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V- Ⅲ by ELISA. Four patients ( 22.2% ) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS. Two Chinese haemophiliacs treated with locally produced factor Ⅷ was sero-negative.The first AIDS patient from abroad died in Beijing in 1985 was also sero-positive.In conclusion ,the data indicated...

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V- Ⅲ by ELISA. Four patients ( 22.2% ) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS. Two Chinese haemophiliacs treated with locally produced factor Ⅷ was sero-negative.The first AIDS patient from abroad died in Beijing in 1985 was also sero-positive.In conclusion ,the data indicated that the Chinese haemophilics were exposed to LAV/HTLV-Ⅲ via imported factor Ⅷ from USA.

对浙江省1982~1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者,用酶联免疫吸附法(ELTSA)检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒(LAV/HTLV-Ⅲ)抗体,发现4例阳性,并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。一例从美国来华旅游死于艾滋病者,LAV/HTLV-Ⅲ抗体阳性。本研究证明,LAV/HTLVⅢ病毒巳通过美国生产的Ⅷ因子制剂传入中国。

Stored donor and recipient sera from prospective studies of post-transfusion hepatitis were analysed for the presence of human T-cell lympbotropic virus type-III/lympha-denopathy associated virus(HTLV-Ⅲ/LAV)

取自输血后肝炎的前瞻性研究中贮存的供者血清及受者血清用酶连接免疫吸附试验(ELISA)测定人T细胞趋淋巴性病毒Ⅲ型/淋巴腺病病毒(HTLV-Ⅲ/LAV)抗体的存在。在输与461名病人的3,961份供者血标本中,经用抗生物素蛋白和生物素加强的Western印迹法测定,仅有2份(0.0%)血标本含有特异性HTLV-Ⅲ/LAV抗体。在输血前,输血后3个月及6个月,分别对以下名类受者测定抗HTLV-Ⅲ/LAV抗体:抗HTLV-Ⅲ阴性血的受者295人,ELISA测定为阳性血(Western印迹法检查为阴性)的受者7人,ELISA测定为阳性血(经Western印迹法证实为特异)的受者2人.以上诸人中,仅最后2名受者经血清抗体转阳试验(P<0.0001)证明感染HTLV-III/LAV,2人血清转化发生于早期(分别为输血后6周及8周),其特征是首先出现抗p24抗体,以后出现抗p41抗体.上述两名病人都未发生获得性免疫缺损综合征(AIDS),但是其中一人Ta/T。比率为。,4,促细胞分裂素的反应也减弱。另一名病人在接触后4年未查见免疫机能不良的证据.这一研究证实HTLV-IB/LA V的感...

取自输血后肝炎的前瞻性研究中贮存的供者血清及受者血清用酶连接免疫吸附试验(ELISA)测定人T细胞趋淋巴性病毒Ⅲ型/淋巴腺病病毒(HTLV-Ⅲ/LAV)抗体的存在。在输与461名病人的3,961份供者血标本中,经用抗生物素蛋白和生物素加强的Western印迹法测定,仅有2份(0.0%)血标本含有特异性HTLV-Ⅲ/LAV抗体。在输血前,输血后3个月及6个月,分别对以下名类受者测定抗HTLV-Ⅲ/LAV抗体:抗HTLV-Ⅲ阴性血的受者295人,ELISA测定为阳性血(Western印迹法检查为阴性)的受者7人,ELISA测定为阳性血(经Western印迹法证实为特异)的受者2人.以上诸人中,仅最后2名受者经血清抗体转阳试验(P<0.0001)证明感染HTLV-III/LAV,2人血清转化发生于早期(分别为输血后6周及8周),其特征是首先出现抗p24抗体,以后出现抗p41抗体.上述两名病人都未发生获得性免疫缺损综合征(AIDS),但是其中一人Ta/T。比率为。,4,促细胞分裂素的反应也减弱。另一名病人在接触后4年未查见免疫机能不良的证据.这一研究证实HTLV-IB/LA V的感染可由输血传播,也支持了对所有供血者做抗HTLV-111/LAV试验是适宜的。研究也证实Western印迹法测定HTLV-III/LAV的特异性是确实可靠的,并提示ELISA试验结果阳性而Western印迹法测定结果为阴性的血液可能没有传染性.

The electrophoretic whole-cell proteinprofiles and McAb recognized moleculesof pathogenic Leptospira interrogans serovarLai strain 017, L. interrogans serovar au-tumnalis strain 606 and nonpathogenic L.biflexa serovar patoc strain patoc I wereanalysed by SDS-PAGE and Western blottechniques. Whole-cell proteins of strains were allseparated into at least 40 distinguishablebands on 10% gel and, the protein profilesof two pathogenic strains were very similar(average similarity = 90%), but were widelydifferent from...

The electrophoretic whole-cell proteinprofiles and McAb recognized moleculesof pathogenic Leptospira interrogans serovarLai strain 017, L. interrogans serovar au-tumnalis strain 606 and nonpathogenic L.biflexa serovar patoc strain patoc I wereanalysed by SDS-PAGE and Western blottechniques. Whole-cell proteins of strains were allseparated into at least 40 distinguishablebands on 10% gel and, the protein profilesof two pathogenic strains were very similar(average similarity = 90%), but were widelydifferent from those of nonpathogenicstrain patoc I (average similarity = 32% and31%). After being transferred from gels tonitrocellulose filters (0.45μm pore size),about 10 to 12 distinct bands of each strainproteins were revealed as antigens bystaining with McAb LB_1(IgG_(2b)) againstL. interrogans serova Lai strain 017 inELISA. The antigenic profiles of bothpathogenic strains were basically identical,but were different at all from that ofnonpathogenic strain with the exceptionof one common molecular of 45000. Our results indicated that characteri-zation of the whole-cell protein profilesand McAb recognized antigen moleculescan reflect the homologous relationshipbetween leptospiral strains, and it may bevaluable in the indenfication of pathogenicand nonpathogenic leptospires.

作者用SDS-PAGE和Western印迹分析了三株钩体全细胞蛋白电泳图谱及McAb识别的抗原分子。致病性问号钩端螺旋体017株、606株蛋白电泳图谱平均相似性为90%,与非致病性双曲钩端螺旋体patoc Ⅰ株相比,分别为32%,31%;与McAb LB_1 识别的抗原图谱相比,017株与606株基本一致,与patoc Ⅰ株均完全不同。提示该分析方法在区分致病性与非致病性钩体上具有一定意义。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关western印迹的内容
在知识搜索中查有关western印迹的内容
在数字搜索中查有关western印迹的内容
在概念知识元中查有关western印迹的内容
在学术趋势中查有关western印迹的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社