Methods ICAM 1 expression of primary cultured human hepatocyte was induced in vitro by IFN γ,TNF α and IL 1, measuring by cellular enzyme linked immunosorbent assay(ELISA).
As a sort of multipotential stem cell, the bone marrow stromal stem cells have been found to be able to differentiate into myogenic cells or cardiomyocytes after induction in vitro.
The production of IL-2 of human peripheral blood mononuclear cells (PBM) stimulated by PHA was studied and the method of IL-2 induction in vitro has been successfully established. IL-2 content was assayed by IL-2 dependent cell-CTLL cell line.
Results BMSCs of rabbits were capable of growth in all 3 groups. After induction in vitro, a calcium node formed on the surface of the scaffold material, and the capacity for adhesion and proliferation were stronger in both experiment group A and B were than that in control group C (P<0.05).
Objective To investigate the feasibility of in vitro inducing differentiation of bone marrow stromal cells(BMSCs) into neural cells by comparison of these two cells.
Objective To study the feasibility of in vitro inducing different of rat bone marrow stromal cells(MSCs) into neurons,and to offer reference for transplantation MSCs for the theaphy of ischemic cerebrovascular disease.
CONCLUSION: In vitro separation, cloning human pancreatic stem cell and inducing pancreatic stem cells into functional islet transplantation for diabetes are effective method of islet donator shortage. But there was no report of human pancreatic stem cells in vitro inducing islet stem cell transplantation for human diabetes.
Leptin response was estimated by measuring the in vitro induction of tis11, a primary response gene activated by STAT3-dependent cytokines in hypothalamic explants after leptin stimulation.
The molecular mechanism of mitochondrial damage under conditions of oxidative stress induced in vitro with low concentrations of FeSO4 and ascorbate (vitamin C) was studied.
We hypothesize that oxidative stress induced in vitro with FeSO4 and millimolar concentrations of ascorbate damages mitochondria by inducing the cyclosporin A-sensitive Ca2+-dependent pore in the inner mitochondrial membrane.
Then, by addition of hydrogen chloride (HCl), self-assembly of IKVAV-PA was induced in vitro and nano-fibers formed as shown by transmission electron microscopy (TEM).
The possibility of hepatotropic infection was also ruled out in that anti-IFNα/β antibody enhanced hepatic but not splenic LAK cell induction in vitro in both conventional and germfree C3H/HeN mice.
After 10?days induction in vitro, prediferentiated ASCs were seeded in an alginate gel system and implanted subcutaneously into the dorsum of nude mice.
MEN 11300, MEN 11301, and MEN 11303 are three recombinant human hybrid proteins that, as has recently been described, induce in vitro erythroid differentiation.
We identified 12 good binders to HLA-DP4 and investigated the capacity of the seven best binders to induce in vitro specific CD4+ T cell lines from HLA-DP4 healthy donors.
In an attempt to induce in vitro differentiation we exposed cells from patients with chronic lymphocytic leukemia (CLL) and with immunocytoma (IC) to 12-0-tetra-decanoylphorbol-13-acetate (TPA) at 160 nM.
Crosslinking membrane immunoglobulin M receptors by F2μ resulted in elevated human leukocyte antigen-DR expression but did not induce in vitro proliferation of PPBL cells.
体外诱导
in vitro induction
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