After incubated with DT389-IL13 for 48 h, there were 38% (1 × 10-9 mol/L) or 99% (1 × 10-8 mol/L) U251 cells undergoing apoptotic changes, while 3.63% apoptotic cells in control.
Result There was no difference between 1.25 nmol/ml FB1 group and blank control group in apoptosis rate. The concentration of 2.5-40 nmol/ml FB1 groups could induce obvious apoptosis and the apoptosis rate increased with the raise of FB1 concentration but 15 nmol/ml FB1 reached the peak.
A typical sub diploid apoptosis peak was demonstrated in lymphocytes treated with DON and AFG 1. A significant dose effect response and time effect correlation could be found between apoptosis rates and mycotoxin concentrations (DON:50～2000 μg/L and AFG 1:3 12～2000 μg/L) and the treated time (DON:2～72 hours and AFG 1:2～24 hours).
The liver's detoxifying function has been preserved.
Concerning the in vivo function of the enzyme, new findings on the physiological role of D-amino acid oxidase point to a detoxifying function of the enzyme in metabolizing exogenous D-amino acids in animals.
Our own results and a literature review led us to reconsider the detoxifying function of MTs in living organisms.
This paper deals with the effect of dysentery intoxication on one detoxifying function of the liver-urea formation-in rats.
Glutathione-S-transferase activity also decreased, which attests to impaired detoxifying function of the placenta.
The recovery effects of 4-aminopyridine,guanidine,tetraethylammonium andamphotericin B on the neuromuscular transmission blocked by type A botulinumtoxin were investigated at the isolated phrenic diaphragm preparation of rat.Itwas found that the spike of motor nerve terminal abolished by botulinum toxinreappeared after application of 4-aminopyridine(2.5×10~(-6))or guanidine(3 mM)to the bathing medium.The amplitude of the spike as well as that of the endplate potential restored progressively.Consequently,the...
The recovery effects of 4-aminopyridine,guanidine,tetraethylammonium andamphotericin B on the neuromuscular transmission blocked by type A botulinumtoxin were investigated at the isolated phrenic diaphragm preparation of rat.Itwas found that the spike of motor nerve terminal abolished by botulinum toxinreappeared after application of 4-aminopyridine(2.5×10~(-6))or guanidine(3 mM)to the bathing medium.The amplitude of the spike as well as that of the endplate potential restored progressively.Consequently,the contraction of nerve-muscle preparation to indirect stimulus also reappeared.The effect of tetraethylam-monium(3 mM)was similar to that of 4-aminopyridine and guanidine but not sopotent as the latters.Amphotericin B(1×10~(-5)),in the presence of 6 mM of Ca~(++),could increase the mean quantum content of the end plate potential but could notrestore the spike of the nerve terminal abolished by botulinum toxin.
The postsynaptic response was recorded from the sixth abdominal ganglion of the cockroach Periplaneata futiginosa with the sucrose-gap technique and the actions of thio-cyclam hydrogen oxalate and a-bungarotoxin (a-bgt) were studied. The results are summarized as follows.1. The excitatory postsynaptic potential (EPSP) was considerably suppressed by thiocyclam hydrogen oxalate at concentrations above 1×10-5M which seemed to be the threshold concentration for the suppressive action when applied on the presynaptic...
The postsynaptic response was recorded from the sixth abdominal ganglion of the cockroach Periplaneata futiginosa with the sucrose-gap technique and the actions of thio-cyclam hydrogen oxalate and a-bungarotoxin (a-bgt) were studied. The results are summarized as follows.1. The excitatory postsynaptic potential (EPSP) was considerably suppressed by thiocyclam hydrogen oxalate at concentrations above 1×10-5M which seemed to be the threshold concentration for the suppressive action when applied on the presynaptic (cercal) nerve.2. When a-bgt was applied to the sixth abdominal ganglion, the EPSP was progressively reduced in amplitude, due to the occupation of acetylcholine receptor by a-bgt. The axon conduction and resting potentials were not affected by thiocyclam hydrogen oxalate or a-bgt. Thus it appears that these two drugs are similar in their mode of action.3. It was seen that thiocyclam hydrogen oxalate frequently induced the bursts of the spontaneous EPSP. The amplitude and frequency of the discharges gradually increased to form spikes and finally the discharges were suppressed.
THe effect of membrane toxin-c (MT-C) on Ca++ -binding and transport in rat liver mitochondria has been studied.The Ca++-binding capacity to the rat liver mitochondria is lowered to an extent of 50% at a concentration of 7. 14 nmole MT-C/ mg mitochondrial protein. Linear relationship has been observed on Scatchard plots of Ca++-binding in the presence of MT-C (Kd: 48.2 uM; number of Ca++-binding sites. 341 nmol/ nag mho.), i. e., MT-C inhibited the high-affinity Ca++-binding sites, but did not interfere with...
THe effect of membrane toxin-c (MT-C) on Ca++ -binding and transport in rat liver mitochondria has been studied.The Ca++-binding capacity to the rat liver mitochondria is lowered to an extent of 50% at a concentration of 7. 14 nmole MT-C/ mg mitochondrial protein. Linear relationship has been observed on Scatchard plots of Ca++-binding in the presence of MT-C (Kd: 48.2 uM; number of Ca++-binding sites. 341 nmol/ nag mho.), i. e., MT-C inhibited the high-affinity Ca++-binding sites, but did not interfere with the low-affinity Ca++-binding sites. Our result suggest that the site of action of MT-C on rat liver mitochondria can be mainly ascribed to the high affinity site of Ca++-binding on the inner membrane.
毒素作用
detoxifying function
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