Exposed to high dosage of DDVP (6.72 mg/L) for 10 d and 20 d, the growth rates of tadpole reduced by 27.0% and 38.8%, respectively, the EST belts also altered and the SOD activity was obviously lower than that of control.
The isozyme proteins of est 1, 2 and est 3, 4, in esterase isozyme loci Est A and Est B, were purified and segregated from mycelia of Agaricus bisporus with polyacrylamide slab gel electrophoresis (PAGE).
Exposed to low dosage of DDVP (2.24 mg/L) for 10 d and 20 d, the growth rates of tadpoles reduced by 24.9% and 9.5%, respectively compared to the control group, the number of esterase isozymes (EST) belts altered, the activity of EST was restrained and the SOD activity changed little.
The difference of LD_(50) value for malathion between the two populations was consistent with the difference of esterase activities, suggesting that general esterases contributed to the malathion resistance of the two populations.
Between them,no significant difference was found in banding patterns of general esterases when α-naphthyl acetate was used as substrate in nondenaturing polyacrylamide gel electrophoresis. However,enzyme kinetics suggested that esterase activity of the females was respectively,1.40-fold,1.30-fold,and 1.39-fold higher than that of the males when α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate were used as substrates.
The results showed that, feeding on clone 7, the activity of esterase, carboxylesterase, and mixed-function oxidases in the midgut of the larvae was very much decreased.
Because carboxyl esterase does not exhibit specificity for these esters, no adaptation period was required for switching the biofilter between biodegradation of methyl acetate and ethyl acetate.
The membrane potential, esterase activity, intracellular pH and production of superoxide anion production were in all four strains considerably modified at high H2O2 concentrations.
The extended pattern of multiple esterase forms has been revealed in the hemolymph of wax moth Galleria mellonellalarvae infected by the fungi Metarhizium anisopliae, Beauveria bassianaor Paecilomyces fumoso-roseus.
The total esterase activity of the hemolymph also increases during mycosis.
Expressed sequence tags (EST) analysis of all putative peroxidase genes of A.
Effects of Genotypes at the Unspecific Esterase (Est) and Leucine Aminopeptidase (Lap) Loci on the Temporal Dynamics of Morpholo
A polymorphism of the total protein (MY) and nonspecific esterase (EST) patterns was revealed in the musculocutaneous sac of earthworm Lumbricus rubellus by polyacrylamide gel electrophoresis.
The revealed polymorphism is attributed to genetically determined codominant systems, each of which is controlled by the corresponding locus, My and Est, represented by two and four alleles, respectively.
Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro