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重组bhk细胞
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  “重组bhk细胞”译为未确定词的双语例句
     Effects of Temperature on Growth,Metabolism and vWF Expression of Recombinant BHK Cells
     温度对重组BHK细胞生长、代谢和vWF蛋白表达的影响
短句来源
     Effects of temperature on the growth,metabolism and vWF expression of recombinant baby hamster kidney(rBHK) cells were investigated in batch and pulse cultures.
     通过重组幼仓鼠肾细胞(BHK细胞)在不同温度下的批培养和脉冲培养,研究了温度对重组BHK细胞生长、代谢以及血管性血友病因子(vWF)蛋白表达的影响。
短句来源
     A new serum-free medium SFMA was formulated using HADAMARD statistical design for the cultivation of rBHK cells and the expression of rec-vWF.
     采用HADAMARD统计设计方法建立了适于重组BHK细胞培养和vWF蛋白表达的无血清培养基SFMA。
短句来源
  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Expression of Recombinant Plasmid pcDNA3.1/P12X3C with Multi-genes of Foot-and-mouth Disease Virus in BHK-21 Cells
     口蹄疫病毒多基因重组质粒在BHK-21细胞中的表达
短句来源
     The plasmid could be expressed by in vitro transfection of BHK cells.
     体外转染BHK细胞可检测到重组质粒的转录表达;
短句来源
     Effects of Temperature on Growth,Metabolism and vWF Expression of Recombinant BHK Cells
     温度对重组BHK细胞生长、代谢和vWF蛋白表达的影响
短句来源
     The construction and in vitro expression of the human growth hormone gene-containing recombination vector
     重组人生长激素基因细胞移植
短句来源
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  recombinant bhk cell
A similar result was obtained for suspended aggregate cultures of both recombinant BHK cell lines.
      


A new serum-free medium SFMA was formulated using HADAMARD statistical design for the cultivation of rBHK cells and the expression of rec-vWF. Comparing with that in (DMEM+FBS(φ_(FBS)=0.05)) and SFM-Ⅱ, rBHK cells grew better in SFMA, and the maximum cell density increased by (16.7%) and 40%, respectively. Moreover, the expression of rec-vWF in SFMA was 18.7% higher than that in (DMEM+FBS(φ_(FBS)=0.05)), and the concentration of rec-vWF in SFMA was also 50% higher than that in SFM-Ⅱ in maintaining cultures of...

A new serum-free medium SFMA was formulated using HADAMARD statistical design for the cultivation of rBHK cells and the expression of rec-vWF. Comparing with that in (DMEM+FBS(φ_(FBS)=0.05)) and SFM-Ⅱ, rBHK cells grew better in SFMA, and the maximum cell density increased by (16.7%) and 40%, respectively. Moreover, the expression of rec-vWF in SFMA was 18.7% higher than that in (DMEM+FBS(φ_(FBS)=0.05)), and the concentration of rec-vWF in SFMA was also 50% higher than that in SFM-Ⅱ in maintaining cultures of rBHK cells in serum-free media.

采用HADAMARD统计设计方法建立了适于重组BHK细胞培养和vWF蛋白表达的无血清培养基SFMA。用SFMA进行重组BHK细胞的批培养,细胞生长明显优于有血清培养(DMEM+FBS(φFBS=0.05))和无血清培养基SFM- 的培养,最大细胞密度可分别提高16.7%和40%。同时,在培养的96h,蛋白的表达水平比有血清培养提高了18.7%,SFMA维持培养48h的vWF蛋白的表达水平比SFM- 提高了50%。

Effects of temperature on the growth,metabolism and vWF expression of recombinant baby hamster kidney(rBHK) cells were investigated in batch and pulse cultures.It was demonstrated that both higher and lower temperatures could retard rBHK cell growth compared with usual 37 ℃,more severe(under) lower temperature.The growth retardation by temperature change is attributed mainly to the prolonging of lag phase,and the specific growth rate of rBHK cells was almost independent on the variation of temperature in exponential...

Effects of temperature on the growth,metabolism and vWF expression of recombinant baby hamster kidney(rBHK) cells were investigated in batch and pulse cultures.It was demonstrated that both higher and lower temperatures could retard rBHK cell growth compared with usual 37 ℃,more severe(under) lower temperature.The growth retardation by temperature change is attributed mainly to the prolonging of lag phase,and the specific growth rate of rBHK cells was almost independent on the variation of temperature in exponential phase.Temperature change had little effect on the cell percentage in S phase of cell cycle,but the cell percentage increased in G_0/G_1 phase and decreased in G_2/M phase obviously.Moreover,the apoptotic cell number of cell population increased with the rising of temperature.The lowering of temperature could remarkably increase vWF expression per cell,the specific production rate of vWF(increased) by 45% at 33 ℃ compared with 37 ℃.

通过重组幼仓鼠肾细胞(BHK细胞)在不同温度下的批培养和脉冲培养,研究了温度对重组BHK细胞生长、代谢以及血管性血友病因子(vWF)蛋白表达的影响。相对于37℃,升高和降低温度的培养都降低了细胞的生长速率和密度,降温的作用更明显,主要表现为延长了细胞生长的迟滞期。在不同温度的脉冲培养中,细胞的比生长速率变化不大,表明进入对数生长期后,细胞生长对一定范围的温度变化不敏感。培养温度对细胞群体中处于S期的细胞比例影响不大,但在33℃和39℃下培养,细胞群体中处于G0/G1期的细胞比例有明显升高,处于G2/M期的细胞比例下降。当培养温度降低到33℃时,细胞群体中发生凋亡的细胞数随着温度升高而增加。温度的降低能显著提高单个细胞的vWF蛋白表达能力,在33℃下培养,vWF平均比生产速率提高了45%。

 
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