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荧光表达
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  fluorescent expression
     The concentration of diluted RV virus solution was determined by counting the NIH3T3 cells with fluorescent expression.
     将稀释的RV病毒液感染NIH3T3细胞,计数NIH3T3荧光表达细胞的数量来确定病毒的浓度;
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     2 When the addictive amount of AS was 200i M in cocultivated medium, the fluorescent expression rate of callus is the highest3 the optimum temperature was 22 C for the agrobacterium-mediated transformation.
     (2)农杆菌共培养培养基中添加AS200μM时,抗性愈伤组织荧光表达率最高。 (3)22℃时共培养,农杆菌转化率最高。
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     Objective This research study was to develop a construct for assay of viral specific expression without affection by high mutation based on the high affinity between the less mutated regulatory protein Rev and Rev response element (RRE) of HIV. Methods Molecular cloning technique was used to synthesize Rev dependant, fluorescent expression plasmid that contained RRE and EGFP. The expression of the new plasmid was further tested by flow cytometry.
     目的 利用人类免疫缺陷病毒 (HIV)低变异的调节蛋白Rev与Rev反应区 (RRE)的高亲和性 ,尝试合成一种不受变异影响的特异性病毒表达检测结构。 方法 采用分子克隆技术合成含有RRE和增强的绿荧光蛋白 (EGFP)的Rev依赖性荧光表达质粒 ,采用流式细胞仪检测新质粒的表达功能。
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     Fluorescent expression of TIMP-1 siRNA was observed in ice tissues pieces of liver,kidney,heart,lung and brain after 48 h,6 d and 12 d.
     结果2d后,P组肝、肾、肺、脑、心肌组织内均有少量TIMP-1siRNA绿色荧光表达,组间无显著差异,P>0.05;
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     Methods In order to construct recombinant attenuated salmonella typhimurium X8786/pEGFP-C3, plasmid pEGFPC3 was translated to attenuated salmonella typhimurium SL8786 by electricity instrument. The recombinant X8786/pEGFP-C3 was fed to six weeks old mice. The EGFP-C3 expression and the organization fluorescent expression in mice were detected by flow cytometry and fluorescent microscopy, respectively.
     方法将质粒pEGFP-C3电转化到减毒鼠伤寒沙门菌SL8786,构建重组减毒鼠伤寒沙门菌X8786(pEGFP-C3),分别灌胃饲服昆明种小鼠,流式细胞仪检测脾脏细胞荧光表达,利用荧光显微镜检测小鼠组织荧光表达
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  fluorescence expression
     the infections of viruses were detected by GFP fluorescence expression;
     rAAV-GFP感染体外培养的兔IPECs,以荧光表达检测病毒的感染情况。
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     Methods After fluorescence expression plasmid pGFP-GR transfected rat alveolar macrophges (AMs), nuclear translocation of GFP-GR following to lipopolysaccharide (LPS) and SNP treatment was observed through fluorescence microscope.
     方法 将GR荧光表达质粒pGFP GR转染大鼠肺泡巨噬细胞,经LPS和SNP作用后,荧光显微镜观察质粒表达产物GFP GR核移位情况;
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     The fluorescence expression in Dorsal Root Ganglia and Anterior Root of the spinal nerves after paraverbural block with
     家兔阿霉素椎旁阻滞后背根神经节及前根运动神经的荧光表达
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     An intense green fluorescence expression was observed in the cells transfected with the plasmid of 5'UTR-EGFP.
     逆转录聚合酶链反应结果与之相符,5′UTR-Luc(+)检测到虫荧光素酶基因 mRNA,而5′UTR- Luc(-)则未检测到。 5′UTR-EGFP(+)观察到较强绿色荧光,而5′UTR-EGFP(-)无荧光表达
短句来源
     Methods rAAV-GFP and rAAV-NAP(containg NT4-NAP fusion gene )were constructed; rabbit IPECs were cultured and infected with rAAV-GFP and rAAV-NAP; transfection of viruses was detected by GFP fluorescence expression;
     方法分离培养兔IPECs,采用非对称互补引物/模板方法构建神经短肽NAP和神经营养因子NT4的融合基因,构建重组腺相关病毒(rAAV)-绿色荧光蛋白(GFP)和rAAV-NAP,并以此转染体外培养的兔IPECs,以荧光表达来检测病毒的转染情况。
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  “荧光表达”译为未确定词的双语例句
     Objective:To study the expression, localization and its relationship with ERK1/2 phosphorylation of Cox7a2 by construction of cox7a2-pEYFP-N1 in TM3 mouse Leydig cells.
     目的:研究Cox7a2的荧光表达载体在TM3睾丸Leydig细胞中的表达和定位及对ERK1/2磷酸化的影响。
短句来源
     Construction and Expression of ATP50 Fluorescent Protein in TM3 Mouse Leydig Cells
     ATP50荧光表达载体构建及其在TM3小鼠睾丸Leydig细胞定位研究
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     N gene coding nucleocapsid (N) protein of PRRSV VR2332 resp strain was amplified by PCR, and cloned in the green fluorescence vector pEGFP-N1. Vector named as "PEGFP-N1-ORF7" with PRRSV VR2332 N gene was obtained by kanamycin screening.
     利用PCR方法扩增出了PRRSV VR2332 resp株的核衣壳蛋白基因(N基因),并克隆进荧光表达载体pEGFP-N1中,利用卡那抗性筛选得到重组阳性克隆,经双酶切鉴定成功后,命名为pEGFP-N1-ORF7。
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     Area of connexin43 expression was higher in group A [(5 325.62±598.90)μm2]than in group B [(4232.33±484.43)μm2]and was correlated to QT interval (r=-0.775). Density of Cx43 expression was not significantly different between two groups.
     Cx43荧光表达面积B组[(4232.33±484.43)μm2]明显于A组[(5 325.62±598.90)μm2],下降约20.5%,与校正Q—T间期相关(r=-0.775),并存在侧边偏移现象。
短句来源
     Methods Cox7a2-pEYFP-N1 plamid was constructed and transfected back to TM3 mouse Leydig cells for the overexpression of Cox7a2.The testosterone secretion was tested by ELISA and the expression of STAR,P450scc and 3β-HSD was investigated by western blotting.
     方法构建Cox7a2荧光表达载体,转染TM3小鼠睾丸Leydig细胞,融合蛋白表达及LH诱导刺激后,ELISA测定细胞上清液中睾酮含量,Western blot检测StAR蛋白、P450scc和3β-HSD酶的表达变化。
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  fluorescent expression
Only cells that fell within the defined gate in the light scatter plot were subsequently analyzed for fluorescent expression.
      
  fluorescence expression
The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection on CD2AP distribution in HEK293 cells.
      
We analyzed the fluorescence expression of structural antigens CD41a (GPII-IIIa) and CD42b (GPIb-V-IX), and of the activation-dependent antigens CD62p (P-selectin, PADGEM, GMP-140) and CD63 (GP53).
      
Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the Selection of Site-Directed Knockout/Knockin
      
After transfection, EGFP fluorescence expression was observed under a confocal laser scanning microscope.
      
To directly confirm GFP expression in intestinal tissues, we next analyzed large intestines for fluorescence expression using immunochemical analysis.
      
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Characteristics of laser induced autofluorescence spectra of tumors have been studied clinically with a detecting system which consists of an argon laser and an optical multichannel analyzer, Through the analysis of endogenous porphyrin specific peaks, 70 cases of malignant tumors, 30 cases of severe dysplasia, 103 cases of benign tumors of chronic inflammation and 600 cases of normal tissue in oral cavity or skin were diagnosed without any fluorescent drug The results showed that the positive rate of malignant...

Characteristics of laser induced autofluorescence spectra of tumors have been studied clinically with a detecting system which consists of an argon laser and an optical multichannel analyzer, Through the analysis of endogenous porphyrin specific peaks, 70 cases of malignant tumors, 30 cases of severe dysplasia, 103 cases of benign tumors of chronic inflammation and 600 cases of normal tissue in oral cavity or skin were diagnosed without any fluorescent drug The results showed that the positive rate of malignant tumors and dysplastic lesions were 86%(60/70) and 97%(29/30), respectively, while all benign lesions in 103 cases and normal mucosa or skin in 600 persons were negative Two cancer foci smaller than 2mm were detected by this procedure During following up by fluorescence analysis for dysplastic lesions, squamous cell carcinoma occurred in two cases, whose specific fluorescence were positive

本文以氩离子激光为激发光源,以光学多道分析仪(OMAⅢ)为光谱分析手段研究恶性肿瘤的激光荧光谱特征。研究中,不给患者任何药物,直接检测病变组织的内源性卟啉荧光,并以这种荧光谱作为特征谱,诊断良恶性疾病。经对203例口腔颌面部、皮肤的良恶性病变和600例正常组织荧光谱检测分析,结果显示:恶性肿瘤中有特征荧光表达的占86%(60/70),中、重度不典型增生性病变中有特征荧光表达的占97%(29/30),而14种良性病变(103例)和600例正常精膜、正常皮肤均未见特征荧光。随访中、重度不典型增生性病变患者,发现2例癌变均为特征荧光阳性者。病例检测中,可以清楚地定位2mm直径的微小癌灶。结果表明本检测方法对提高恶性肿瘤早期诊断率是很有意义的。

Objective To explore the regulating effects of radiation inducible gene on the expression of hematopoiedtic growth factor genes Methods The human FLT3 Ligand (FL) cDNA and EGFP cDNA were linked together with IRES and then inserted into the eukaryotic expression vector pCIEgr, which was constructed by substituting CMV promoter in pCIneo with the Egr1 promoter(EgrEF) The expression of FL in HFCL/EF cells were confirmed with RTPCR、ELISA、FACS and...

Objective To explore the regulating effects of radiation inducible gene on the expression of hematopoiedtic growth factor genes Methods The human FLT3 Ligand (FL) cDNA and EGFP cDNA were linked together with IRES and then inserted into the eukaryotic expression vector pCIEgr, which was constructed by substituting CMV promoter in pCIneo with the Egr1 promoter(EgrEF) The expression of FL in HFCL/EF cells were confirmed with RTPCR、ELISA、FACS and cell culture Results The activity of EGFP in transfected cells increased after exposure to 25Gy The amounts of secreted FL in serumfree supernatants of HFCL/EF were significantly higher than the control group FL cDNA was successfully expressed in the cells by ELISA and RTPCR analysis At day 10 of culture the number of CD34+ cells in HFCL/EF culture supernatants was significantly higher than that of nonradiation group ConclusionThese results showed that radiation can enhance the ability of the supernatants containing FL of HFCL/EF to expand early hematopoietic progenitor cells and protect hematopoietic cells from radiationinjury effects [

目的探索辐射诱导基因调控序列启动造血生长因子基因表达及观察其对造血恢复的作用。方法本实验将带有 Egr- 1调控序列启动的 FL T3配基 ( FL )和 EGFP双顺反子基因表达载体 ( Egr- EF)转染骨髓基质细胞系 HFCL (称 HFCL /EF) ;用 RT— PCR鉴定细胞内目的基因的 m RNA表达 ;采用 FACS观察 EGFP绿色荧光表达的阳性细胞 ;用 EL ISA方法检测 HFCL / EF细胞培养上清 FL的含量 ;观察 HFCL / EF培养上清液对 CD34+细胞的增殖作用。结果在 HFCL / EF细胞中证实有外源性基因 EGFP和 FL 的整合和表达 ,在辐照 16 h后的 HFCL/ EF细胞培养上清液中表明 FL 含量较照射前明显增高( P<0 .0 1) ;同时证实辐射 10 d后 HFCL/ EF培养上清液对 CD34+造血祖细胞的作用较辐射前具有明显的扩增作用 ( P<0 .0 1)。结论 Egr- 1调控序列启动的造血生长因子基因在辐射后表达明显增高并促进造血祖细胞增殖作用

Purpose:To study the establishment of stable,high level green fluorescent protein (GFP) expressing cell line of hepatocellular carcinoma(HCC) and pass on its culture contiuously.Methods:7721 cell line of HCC was transfected by retrovirus vector with integrated GFP cDNA. GFP expressing cancer cells were detected by fluorescent microscopy through G418 selecting and cloned culture.Results:Most of the cancer cells were dead 5 days after being transfected by GFP, scattered or clustered green fluorescence can be...

Purpose:To study the establishment of stable,high level green fluorescent protein (GFP) expressing cell line of hepatocellular carcinoma(HCC) and pass on its culture contiuously.Methods:7721 cell line of HCC was transfected by retrovirus vector with integrated GFP cDNA. GFP expressing cancer cells were detected by fluorescent microscopy through G418 selecting and cloned culture.Results:Most of the cancer cells were dead 5 days after being transfected by GFP, scattered or clustered green fluorescence can be seen by microscopy. In culture for 65 days,almost all of the clone 28 cells expressed high intensity GFP fluorescence and stability.Conclusions:7721 GFP cell line can provide a basis for establishing an ideal animal model for research of tumor invasion and metastasis.

目的 :研究建立稳定高表达绿色荧光蛋白 (GFP)并能连续传代培养的肝癌细胞株。方法 :将携带GFPcDNA的重组逆转录病毒载体转染肝癌细胞株 772 1,经过G418筛选和克隆化培养 ,用荧光显微镜检测癌细胞荧光表达。结果 :转染GFPcDNA的癌细胞 5d后大部分死亡 ,荧光显微镜下有散在或小团状的点状荧光。继续用G418筛选及克隆化培养 6 5d后第 2 8培养孔的癌细胞几乎均见荧光表达并可连续稳定传至 15代以上的培养。结论 :GFP是较好的细胞报告基团 ,肝癌 772 1 GFP细胞株的建立有助于观察和了解肿瘤侵袭和转移发生及其规律。

 
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