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高压修复
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  “高压修复”译为未确定词的双语例句
     Results Using pressure cooker, the best signal for ER and PR was observed after retrieval in the EDTA buffer(pH 8.0) for 5 min.
     结果:高压修复时,ER和PR最适的条件是采用pH值8.0的EDTA修复液煮沸喷气后持续5min,其染色的背景清晰,无非特异性着色。
短句来源
     the group of DW showed all negative. 0.55 of HP group was positive, which was higher than 0.28 of MW group and 0.15 of SB group ( P <0.05 and P <0.01).
     高压修复阳性率 0 .5 5 >微波修复 0 .2 8和煮沸修复 0 .12 (P <0 .0 5 ) .
短句来源
     Conclusion AP-LsAB and high pressure cooking retrieval were recommended for use in immunohistochemical staining of pulmonary inflammatory tissue.
     结论 在肺组织免疫组化染色中推荐使用 AP- L s AB法 ,并采用高压修复抗原。
短句来源
     Streptavidin-peroxidase (SP) and high pressure immunohistochemical methods were adopted to examine the PPARγ.
     采用链霉菌抗生物素蛋白-过氧化物酶(SP)法加高压修复的免疫组化法进行检测。
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  相似匹配句对
     Repair of the Cylinders of High Pressure Heat Exchangers in Fabrication
     在制高压换热器筒体的修复
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     Repair of Upper Shell Cover 3203-C in High-pressure Syringe of Urea Production
     尿素高压洗涤器3203-C上封头修复
短句来源
     HIGH PRESSURE HEAT EXCHANGER
     高压换热器
短句来源
     About Repair Books in Library
     论图书修复
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     The Ways Repairing Dies
     修复模具的途径
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  pressure method
By means of maximal bubble pressure method, the dynamic surface tensions of aqueous octanoyl-N-methylglucamine (Mega-8) solutions at 25°C were measured.
      
The adsorption kinetics of MEGA-12 was studied by the maximum bubble pressure method.
      
The density and surface tension of melts of the systems CaO-FeO-Fe2O3 MgO at the temperature 1623 K, CaO-FeO-Fe2O3-ZnO at 1573 K, and CaO-Fe2O3-Cu2O at 1573 K were determined using the maximum bubble pressure method.
      
The mechanism of materials modification of HPCH is explained from the microscopic point of view by testing the pore structure and pore distribution via the mercury intrusion pressure method.
      
High pressure method was used for the first time to produce rectorite clay (REC)/phenolic resin (PF) and organic rectorite clay (OREC)/phenolic resin and montmorillonite (MMT)/phenolic resin (PF) nanocomposites.
      
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AIM To study the ideal method of retrieving the antigenic activity of BCL 6 in paraffin sections and thepossible mechanism of the antigen masking. METHODS Paraffin embedded sections from 11 cases of lymphoid reactive hyperplasia were retrieved respectively by four retrieving solutions with different combining power with calcium: EDTA, citrate, sodium carbonate (SC) and deionized water (DW), which with different retrieval methods: high pressure (HP), microwaving (MW) and stove boiling (SB), and the antigenic...

AIM To study the ideal method of retrieving the antigenic activity of BCL 6 in paraffin sections and thepossible mechanism of the antigen masking. METHODS Paraffin embedded sections from 11 cases of lymphoid reactive hyperplasia were retrieved respectively by four retrieving solutions with different combining power with calcium: EDTA, citrate, sodium carbonate (SC) and deionized water (DW), which with different retrieval methods: high pressure (HP), microwaving (MW) and stove boiling (SB), and the antigenic activities were detected by immunohistochemistry. RESULTS The positive percentage in sections retrieved by EDTA, citrate and SC were 0.73, 0.45 and 0.12 respectively. The difference among the groups was significant ( P < 0.01 ); the group of DW showed all negative. 0.55 of HP group was positive, which was higher than 0.28 of MW group and 0.15 of SB group ( P <0.05 and P <0.01). The positive incidences of sections retrieved by HP with EDTA and HP with citrate were 1 and 0.82 respectively, which were higher than HP with SC (0.36) and HP with DW (0) ( P < 0.01). 0.82 of HP with EDTA was intensive positive, the difference in positive intensity between HP with EDTA group and above mentioned groups was significant ( P < 0.01 ). CONCLUSION HP with EDTA is the ideal method for retrieving antigenic activity of BCL 6 by formalin fixative, suggesting that calcium may be involved in the antigen masking.

目的 探讨石蜡切片中BCL 6抗原失活机制及修复的最佳方法 .方法 淋巴组织反应性增生 11例石蜡切片分别用二乙胺四乙酸 (EDTA)、柠檬酸、碳酸钠及去离子水 4种与钙离子结合力不同的修复液 ,结合高压、微波和煮沸 3种不同方法修复后 ,比较检出的BCL 6阳性率及强度 .结果 BCL 6阳性率由高至低依次为 :EDTA 0 .73>柠檬酸 0 .45 (P<0 .0 1) >碳酸钠 0 .12 (P <0 .0 1) ,去离子水组无 1例阳性 .高压修复阳性率 0 .5 5 >微波修复 0 .2 8和煮沸修复 0 .12 (P <0 .0 5 ) .不同修复液与高压结合修复检出BCL 6阳性率及强度 :EDTA与柠檬酸阳性率分别为 1,0 .82 (P >0 .0 5 ) ,高于碳酸钠组 (0 .36 )和去离子水组 (0 ) (P <0 .0 1) .EDTA组强阳性占 0 .82 ,显著高于其他各组 (P <0 .0 1) .结论 EDTA结合高压是修复BCL 6抗原的理想方法 ,钙离子的参与可能是甲醛固定后BCL 6抗原失活的原因之一

Objective To compare two kinds of labeled streptavidin biotin(LsAB) methods and provide the optimal method of antigen retrieval. Methods The alkaline phosphatase(AP) LsAB method and the horseradish peroxidase(HRP) LsAB method were used to stain anti-tryptase in paraffin embedding tissue of patients with chronic obstructive pulmonary disease(COPD) and their staining effects were compared. The antigens were repaired by high pressure cooking,tryptin digestion and microwave,and the repairing effects were compared....

Objective To compare two kinds of labeled streptavidin biotin(LsAB) methods and provide the optimal method of antigen retrieval. Methods The alkaline phosphatase(AP) LsAB method and the horseradish peroxidase(HRP) LsAB method were used to stain anti-tryptase in paraffin embedding tissue of patients with chronic obstructive pulmonary disease(COPD) and their staining effects were compared. The antigens were repaired by high pressure cooking,tryptin digestion and microwave,and the repairing effects were compared. Results The backgrounds of stained sections were more distinct and the color distinction of nucleus and positive signal were brighter by AP-LsAB, compared with those by HRP-LsAB; the repairing effect of high pressure cooking was better than the repairing effect of tryptin digestion and than that of microwave. Conclusion AP-LsAB and high pressure cooking retrieval were recommended for use in immunohistochemical staining of pulmonary inflammatory tissue.

目的 比较两种标记生物素链亲和素法 (L s AB) ,并探讨抗原修复的最佳方法。方法 采用碱性磷酸酶 (AP) L s AB法和辣根过氧化物酶 (HRP) L s AB法染色肺癌合并慢性阻塞性肺疾病 (COPD)患者肺组织石蜡切片中类胰蛋白酶 (tryptase)的抗肥大细胞类胰蛋白酶抗体 (AA1,小鼠抗人 ) ,并比较染色效果 ;用高压、胰蛋白酶和微波三种方法进行抗原修复 ,比较修复效果。结果  AP- L s AB法染色比 HRP- L s AB法背景清晰、颜色对比鲜明 ;高压修复效果优于胰蛋白酶和微波修复。结论 在肺组织免疫组化染色中推荐使用 AP- L s AB法 ,并采用高压修复抗原。

OBJECTIVE:Tissue microarray(TMA) is a novel array-based high-throughput technique that facilitates analysis of very large numbers of tumor tissues with hith-efficiency. The authors recently developed the TMA technology and its applications with immunohistochemistry (IHC). METHODS: Optimizing of IHC and immunofluorescence (IF) with TMA technology and comparison with traditional pathology slides.RESULTS: Tissue dots of TMA we orderly arrayed and uniformed in size with good H&E staining. The rate of incomplete...

OBJECTIVE:Tissue microarray(TMA) is a novel array-based high-throughput technique that facilitates analysis of very large numbers of tumor tissues with hith-efficiency. The authors recently developed the TMA technology and its applications with immunohistochemistry (IHC). METHODS: Optimizing of IHC and immunofluorescence (IF) with TMA technology and comparison with traditional pathology slides.RESULTS: Tissue dots of TMA we orderly arrayed and uniformed in size with good H&E staining. The rate of incomplete tissue dots and corresponding positive detection were 23.81%、68.25%,20.63%、77.78%,11.11%、87.30%,11.11%、92.06% respectively. The positive detection rate for tumor cell nucleus antigens was higher by high pressure antigen retrieve method than that by microwave method,P<0.05. Similar results of TMA also was obtained for cell nucleolus and cytoplasm antigens by IF . No significant difference tumor markers detection rate was obtained between TMA and traditional pathology slides,P>0.05. There were different expression level of various tumor markers of TMA. CONCLUSIONS:TMA antigen retrieve protocols can be chosen according to individual antigen. Better results can be obtained with high pressure antigen retrieve in IF. Pathological information of TMA basically represents traditional slides. TMA in situ analysis of gene and protein expression significantly save time and cost and increase efficiency.

目的 :组织芯片是新的高通量分析技术 ,具有样本量大、高效、快速等特点。本研究建立组织芯片技术及其相关的免疫组化实验方法 ,并探讨其实际应用价值。方法 :在组织芯片技术的基础之上进行免疫组化和免疫荧光染色 ,同时对各种实验条件进行选择和优化 ,以优化的实验方法检测各种肿瘤标志物的并与传统病理切片技术进行了比较。结果 :制做的组织芯片排列整齐 ,组织点大小一致 ,HE染色均匀。用煮沸、酶消化、微波和高压法进行抗原修复其残点缺点率和检测阳性率分别为2 3 81% ( 15 / 63 )、 68 2 5 % ( 4 3 / 63 ) ,2 0 63 %( 13 / 63 )、77 78% ( 4 9/ 63 ) ,11 11% ( 7/ 63 )、87 3 0 %( 5 5 / 63 ) ,11 11% ( 7/ 63 )、92 0 6% ( 5 8/ 63 )。高压修复法对细胞核抗原的检测阳性率高于微波修复法 ,P <0 0 5。免疫荧光法检测组织芯片上的细胞核或浆抗原也可以得到较好的检测结果。用组织芯片检测多种肿瘤标志物其表达阳性率与大病理切片的检测结果基本相同 ,P >0 0 5。不同或...

目的 :组织芯片是新的高通量分析技术 ,具有样本量大、高效、快速等特点。本研究建立组织芯片技术及其相关的免疫组化实验方法 ,并探讨其实际应用价值。方法 :在组织芯片技术的基础之上进行免疫组化和免疫荧光染色 ,同时对各种实验条件进行选择和优化 ,以优化的实验方法检测各种肿瘤标志物的并与传统病理切片技术进行了比较。结果 :制做的组织芯片排列整齐 ,组织点大小一致 ,HE染色均匀。用煮沸、酶消化、微波和高压法进行抗原修复其残点缺点率和检测阳性率分别为2 3 81% ( 15 / 63 )、 68 2 5 % ( 4 3 / 63 ) ,2 0 63 %( 13 / 63 )、77 78% ( 4 9/ 63 ) ,11 11% ( 7/ 63 )、87 3 0 %( 5 5 / 63 ) ,11 11% ( 7/ 63 )、92 0 6% ( 5 8/ 63 )。高压修复法对细胞核抗原的检测阳性率高于微波修复法 ,P <0 0 5。免疫荧光法检测组织芯片上的细胞核或浆抗原也可以得到较好的检测结果。用组织芯片检测多种肿瘤标志物其表达阳性率与大病理切片的检测结果基本相同 ,P >0 0 5。不同或同一种分子标志物在各种不同肿瘤组织中的表达有差异。结论 :用组织芯片检测不同的抗原时应选择不同的抗原修复方法。用免疫荧光法检测组织芯片上的细胞核或质抗原时最好选择高压修复法。组织芯片上的组织点基本可以代表传统病理?

 
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