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家兔阴茎海绵体
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  rabbit corpus cavernosum
     Isolated segments of rabbit corpus cavernosum were exposed to increasing concentration of Ber, the accumulation of cGMP were determined in the tissue samples by means of ()~(125)I radioimmunoassay.
     应过125I放射免疫测定法,测定不同浓度的Ber对离体家兔阴茎海绵体平滑肌组织中cGMP水平的影响。
短句来源
     Methods: Isolated segments of the rabbit corpus cavernosum were exposed to different concentrations of Ber, and the dosage-dependent accumulations of cGMP and cAMP were determined in the tissue samples by means of ~ 125I radioimmunoassay.
     方法:采用125I放射免疫测定法,以西地那非(sildenafil,Sil)为阳性对照,测定不同浓度的Ber对离体家兔阴茎海绵体平滑肌组织中cGMP和cAMP水平的影响。
短句来源
     Objective: To further investigate the action mechanisms of berberine (Ber), and assess the effects of Ber on the in vitro formation of cGMP and cAMP in the isolated rabbit corpus cavernosum.
     目的:观察小檗碱(berberine,Ber)对离体家兔阴茎海绵体平滑肌组织中环磷酸鸟苷(cGMP)和环磷酸腺苷(cAMP)水平的影响。
短句来源
     Lot and zaprinast(Zap)enhanced the cyclic GMP level in rabbit corpus cavernosum in concentration dependent manner. This effect of Lot was much weaker than that of Zap.
     在家兔阴茎海绵体 ,Lot和扎普司特 (Zap)可剂量依赖性地增加海绵体cGMP含量 ,Lot的作用远弱于Zap。
短句来源
     Culture and Identification of Human and Rabbit Corpus Cavernosum Smooth Muscle Cells
     成人及家兔阴茎海绵体平滑肌细胞培养和鉴定
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  “家兔阴茎海绵体”译为未确定词的双语例句
     Results The basal concentration of cAMP in rabbit's corpus carvernosum tissue is(5.67±0.97) pmol/mg.
     结果家兔阴茎海绵体组织中cAMP基础含量为(5.67±0.97)pmol/mg。
短句来源
     The basal concentration of cGMP in rabbit's corpus carvernosum tissue is(0.44±0.09)pmol/mg.
     家兔阴茎海绵体组织中cGMP基础含量为(0.44±0.09)pmol/mg。
短句来源
     Effects of Telramethylpyraze on Cytosolic Free Calcium Concentration in Penis Corpus Cavernsum Smooth Muscle Cells in Rabbits
     川芎嗪对家兔阴茎海绵体平滑肌细胞游离钙浓度的影响
短句来源
     Objective: To study the effects of Chinese medicine telramethylpyraze (TMP) on intracellular free calcium (\[Ca 2+ \] i ) in cultured penis corpus cavernsum smooth muscle cell (PCSMC) in rabbits.
     目的 :研究中药川芎嗪 (TMP)对体外培养家兔阴茎海绵体平滑肌细胞 (PCSMC)胞质内游离钙浓度([Ca2 + ] i)的影响。
短句来源
     Methods: Observations were made on the relaxing effect of Chuanxiongzine on phenylephrine (PE) precontractedcorpus cavernosum and cavernosal strips preincubated with L NAME, and ODQ, using endothelium removal and experimental method of smooth muscle strips.
     方法 :采用离体家兔阴茎海绵体肌条张力记录法 ,观察川芎嗪对去氧肾上腺素 (phenylephrine,PE)诱导收缩的阴茎海绵体肌条的舒张作用 ; 应用亚硝基左旋精氨酸甲酯 (L NAME)、ODQ预处理和去除内皮 ,分别记录川芎嗪对阴茎海绵体肌的舒张作用。
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  相似匹配句对
     Effect of Icarrin on cGMP levels in penile corous cavernosum of rabbit
     淫羊藿苷对家兔阴茎海绵体cGMP浓度的效果
短句来源
     Relaxant Effects of Icariin on the Rabbit Corpus Cavernosum and Its Mechanism
     淫羊藿苷对家兔阴茎海绵体的舒张作用及机制
短句来源
     Effects of Icariin on Cyclic GMP and Cyclic AMP Levels in the Rabbit Corpus Cavernosum in vitro
     淫羊藿苷对家兔阴茎海绵体环核苷酸水平的影响
短句来源
     Culture and Identification of Human and Rabbit Corpus Cavernosum Smooth Muscle Cells
     成人及家兔阴茎海绵体平滑肌细胞培养和鉴定
短句来源
     Comparison of rabbit corporal smooth muscle cell culture methods in vitro
     家兔阴茎海绵体平滑肌细胞体外培养方法对比研究
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  rabbit corpus cavernosum
Effects of ligustrazine on the contraction of isolated rabbit corpus cavernosum strips
      
To investigate the role of ligustrazine on relaxation of the isolated rabbit corpus cavernosum tissuein vitro, the effects of ligustrazine on the corpus cavernosum were observed by using experimental method of smooth muscle strips.
      
To further investigate the mechanisms of action of icariin (ICA), we assessed the effects of ICA on the in vitro formation of cGMP and cAMP in isolated rabbit corpus cavernosum.
      
Isolated segments of rabbit corpus cavernosum were exposed to increasing concentrations of ICA and the dose-dependent accumulation of cGMP and cAMP was determined in the tissues samples by means of 125I radioimmunoassay.
      
For the ex-vivo study, the relaxation effects of resveratrol on isolated New Zealand white rabbit corpus cavernosum, precontracted by phenylephrine (5×10-5 M) were measured.
      
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AIM To investigate the effect of Icarrin on penile erection and cyclic GMP levels in rabbit corpus cavernosum(RCC). METHODS Effects of various concentration of Icariin on cGMP level in RCC were measured by 125 I radioimmunoassay procedure with Sildenafil and papaverine as the positive controls RESULTS In the presence of SNP, Icariin increased cGMP concentration in rabbit corpus cavernosum in a dose dependent manner ( P <0 01). EC 50 of Icariin was 5 13 μmol·L -1 (while EC 50...

AIM To investigate the effect of Icarrin on penile erection and cyclic GMP levels in rabbit corpus cavernosum(RCC). METHODS Effects of various concentration of Icariin on cGMP level in RCC were measured by 125 I radioimmunoassay procedure with Sildenafil and papaverine as the positive controls RESULTS In the presence of SNP, Icariin increased cGMP concentration in rabbit corpus cavernosum in a dose dependent manner ( P <0 01). EC 50 of Icariin was 5 13 μmol·L -1 (while EC 50 of sildenafil and papaverine was: 0 31 μmol·L -1 and 3 15 μmol·L -1 ) respectirely. CONCLUSIONS The effect of Icariin on penile erection may be mediated by increasing cGMP levels in CC for enhancing the relaxation response of CC.

目的 观察淫羊藿苷对家兔阴茎海绵体组织中cGMP浓度的影响。方法 应用12 5I放射免疫法 ,测定不同浓度的淫羊藿苷对阴茎海绵体内PDE5酶底物cGMP浓度的影响 ,并以西地那非和罂粟碱作为阳性对照。结果 淫羊藿苷能提高阴茎海绵体内cGMP浓度 ,具有浓度依赖性 (P<0 0 0 1) ,其半数有效浓度 (EC50 )为 5 13μmol·L-1,而西地那非 0 31为 μmol·L-1,罂素碱为 3 15 μmol·L-1。结论 淫羊藿苷对阴茎勃起的作用机制与其能提高海绵体平滑肌cGMP浓度而增强阴茎海绵体平滑肌松弛作用有关

AIM To study the inhibitory effect and selectivity of lotusine (Lot), an alkaloid extracted from Nelumbo nucifera Gaertn., on phosphodiesterase Ⅲ or Ⅴ. METHODS To use radioimmunoassay and recording of contractile force. RESULTS Lot (0.1-3 μmol·L -1 ) and amrinone (Amr, 0.1-10 μmol·L -1 ) concentration dependently increased the cyclic AMP level of the rat myocardium. The effect of Lot was stronger than that of Amr. Lot and zaprinast(Zap)enhanced the cyclic GMP level in rabbit corpus cavernosum in...

AIM To study the inhibitory effect and selectivity of lotusine (Lot), an alkaloid extracted from Nelumbo nucifera Gaertn., on phosphodiesterase Ⅲ or Ⅴ. METHODS To use radioimmunoassay and recording of contractile force. RESULTS Lot (0.1-3 μmol·L -1 ) and amrinone (Amr, 0.1-10 μmol·L -1 ) concentration dependently increased the cyclic AMP level of the rat myocardium. The effect of Lot was stronger than that of Amr. Lot and zaprinast(Zap)enhanced the cyclic GMP level in rabbit corpus cavernosum in concentration dependent manner. This effect of Lot was much weaker than that of Zap. Lot, papaverine (Pap),Zap and Amr obviously inhibited the rabbit corpus cavernosum contractile response induced by phenylephrine (10 μmol·L -1 ). The -lg EC 50 (mol·L -1 )of them was Zap(5)>Pap(4.9)>Amr(4.6)>Lot (3.7) respectively. CONCLUSION Lot selectively inhibits phosphodiesterase(PDE)Ⅲ activity and maybe a novel PDE Ⅲ inhibitor.

目的 研究洛土辛 (Lot)对磷酸二酯酶 (PDE)Ⅲ或Ⅴ亚型的抑制作用及选择性。方法 采用放射免疫测定法及收缩张力记录。结果 Lot和氨力农(Amr)剂量依赖性地增加大鼠心肌cAMP含量 ,且Lot的作用强于Amr。在家兔阴茎海绵体 ,Lot和扎普司特 (Zap)可剂量依赖性地增加海绵体cGMP含量 ,Lot的作用远弱于Zap。Lot,罂粟碱 (Pap)、Zap及Amr可显著抑制预先用苯肾上腺素诱发的阴茎海绵体血管收缩 ,其EC50 分别依次为Zap >Pap >Amr >Lot。结论 提示洛土辛的作用机理可能与选择性的抑制PDEⅢ有关。

Objective To apply series of convenient and effective methods to produce large amount of pure corporal smooth muscle cells in vitro in terms of experimental requirement.Methods The explant cell culture and digestion cell culture methods were compared.Results The cell yield and purity were significantly higher in digestion cell culture compared with explant cell culture;cell growth velocity was more rapid in digestion cell culture also;but the cell culture success rates were higher in explant cell culture...

Objective To apply series of convenient and effective methods to produce large amount of pure corporal smooth muscle cells in vitro in terms of experimental requirement.Methods The explant cell culture and digestion cell culture methods were compared.Results The cell yield and purity were significantly higher in digestion cell culture compared with explant cell culture;cell growth velocity was more rapid in digestion cell culture also;but the cell culture success rates were higher in explant cell culture compared with digestion cell culture and the explant cell culture method was much easier to be mastered.Conclusions Each method had individual merit and shortcoming.The best one of the two methods could be chosen according to experimental requirement.The SMC cultured in vitro are proved to be used to evaluate and investigate the effect of some medicine on penile erection.

目的 探索更好、更方便的家兔阴茎海绵体平滑肌细胞体外培养方法。方法 分别采用组织块法、酶消化法、组织块 +酶消化法对家兔阴茎海绵体平滑肌细胞进行培养 ,在倒置显微镜下对培养过程的细胞分别作了生长情况及形态学观察。用HE染色、MTT法分别描绘原代培养细胞的生长曲线、细胞分裂指数曲线 ;用倒置显微镜观察活细胞的贴壁过程 ,计数法测定细胞贴壁率。结果 组织块法及酶消化法培养的细胞其生长状况和形态学各有自己的特点。组织块法的细胞生长慢 ,培养时间相对较长 ,纯度相对较高 ,原代培养细胞生长速度快 ,但由于该法消化时间不易掌握 ,常影响其成功率 ,多数细胞呈梭形 ,细胞的密度高 ,原代培养时间为 7~ 10d。结论 每种培养方法都有各自的优缺点。我们可根据实验的需要而选择不同的方法或联合培养

 
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