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受鼠
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  recipient mice
     Recipient mice in Groups 2-6 which received lethal irradiation at dose of 9.0 Gy five hours before the transplantation were infused 1×10~5 CD34~+ cells and 1×10~7 TK~+T lymphocytes via tail veins.
     第2~6组移植前5h给予9.0Gy致死量照射,每只受鼠自尾静脉注入1×105个CD34+细胞和1×107个TK+T细胞。
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     Results: All recipient mice in CD34++TK+T group manifested acute GVHD symptoms and survived for 13.3±1.2 days. WBC of recipient mice were 1.76±0.25×109/L before death. Pathological manifestations of CD34+ +TK+T group were quite different from those of irradiated group.
     结果CD34++TK+T细胞组均发生急性GVHD,生存时间13.3±1.2天,受鼠死亡前外周血白细胞1.76±0.25×109/L,病理表现与单纯照射组明显不同。
短句来源
     Both were mixed and transfused into female F1 recipient mice,CD34 + cells and TK + T lymphocytes each F1 recipient mouse received were 1×10 5 and 1×10 7,respectively.
     两者混合移植入雌性F1代受鼠 ,每只受鼠输入CD34+ 细胞和TK+ T细胞数分别为 1× 10 5个和 1× 10 7个。
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     Methods Both BMMSC obtained after three to four weeks of culture and bone marrow cells of donor mice C57BL/6(H-2~b) were transplanted into the recipient mice BalB/c(H-2~d) that were lethally irradiated.
     方法将供鼠C57BL/6(H-2b)的骨髓细胞和体外培养的BMMSC联合输给致死量照射的受鼠BalB/c(H-2d)。
短句来源
     The both were mixed and infused into female F1 recipient mice, CD34+ cells and TK+T lymphocytes each F1 recipient mouse received were 1×105 and 1×107, respectively.
     两者混合移植雌性F1代受鼠,每只受鼠输入CD34+细胞和TK+T细胞分别为1×105和1×107。
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     Methods The murine model of MHC haploidentical BMT was established by using (0576176×BALB/c) BCF_1 (H-2~(d-b)) mouse as the donor, and BALB/c( H-2~d) mouse as the recipient.
     方法以(C257BL/6×BALB/c)BCF_1(H-2~(d/b+))为供鼠,BALB/c(H-2~d)为受鼠建立小鼠MHC半相合BMT模型。
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     The serum concentrations and mRNA expressions of IL-2 and TNFα in spleens decreased significantly(P<0.05);
     同时,雷公藤多甙可降低受鼠血清中IL-2、TNFα浓度和脾组织IL-2、TNFα的mRNA的表达(P<0.05);
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     METHODS: An acute graft versus host disease (aGVHD) mice model was established using C57B/6 mice as donors and BALB/c mice as recipients.
     方法以C57B/6小鼠为供鼠,BALB/c小鼠为受鼠,建立小鼠异基因骨髓移植的aGVHD模型;
短句来源
     (Results) The graft growth in all 29 recipient animals was observed, and the mass of grafts was significantly increased from (0.73)±(0.05) mm in diameter and (5.67)±(0.72) mg in wet weight pre-grafting to (6.75)±(0.73) mm and (113.12)±(78.23) mg post-grafting, respectively.
     结果术后29只受鼠全部存活,在其背部均观察到移植物的生长,移植物直径由移植前的(0.73±0.05)mm增加到(6.75±0.73)mm,湿重由移植前(5.67±0.72)mg增加到(113.12±78.23)mg,受鼠皮肤血管已广泛深入到移植物内;
短句来源
     Donor mice BALB/C female , receptor mice BALB/C , C57 BL/6male ,10-12 weeks , about 20g .
     供鼠BALB/C,雌性,受鼠BALB/C,C57BL/6,雄性,均为8-12周龄,体重20克左右。
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  相似匹配句对
     In this thesis, inspired by J.
     J.
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     Afterwards we observed GVHD of each groups .
     观察各GVHD反应。
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     Every group included 6 recipients.
     每组 6只。
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     ③The disease is typically characterized by replacement of the myocardium of right ventricle wall by the fibrofatty tissue.
     ③?
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     Mice Wandering around the World
     傲江湖
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  recipient mice
Under conditions of tissue therapy of the wound, the formation of muscle fibers from muscle cells of the graft and active regeneration of muscle fibers in the recipient mice were observed, and no structural defects were detected in the thymus.
      
The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F, pups were born.
      
Furthermore, both L3T4+ (CD4) and Lyt2+ (CD8) T cell lines can confer a certain degree of adoptive protection upon naive recipient mice.
      
A more successful strategy is retroviral transduction of BCR/ABL into mouse bone marrow in vitro, followed by transplantation into syngeneic or immunodeficient recipient mice.
      
Recent studies in animal models have demonstrated that isolated testicular germ cells collected from testes may be transplanted into sterile recipient mice to regenerate spermatogenesis.
      
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The diffusion chamber culture in vivo provides a nearly natural condition for the growth of cells. In the present study, the growth process of leukemic cells from 4 strains of leukemic mice (L615, L7212, L1210, P388) was observed dynamically. After a period of exponential growth, cell growth peak can be reached on the 6th or 7th day of culture, and the cell numbers were 5-14 folds of the inoculated cells. If anti-tumor agents were given to the recipients orally or intra-muscularly, the tumor cells would be affected...

The diffusion chamber culture in vivo provides a nearly natural condition for the growth of cells. In the present study, the growth process of leukemic cells from 4 strains of leukemic mice (L615, L7212, L1210, P388) was observed dynamically. After a period of exponential growth, cell growth peak can be reached on the 6th or 7th day of culture, and the cell numbers were 5-14 folds of the inoculated cells. If anti-tumor agents were given to the recipients orally or intra-muscularly, the tumor cells would be affected in accordance with their sensitivity to the anti-tumor agents. The sensitivities of P388 leukemic cells cultured in diffusion chamber to Ⅱ anti-tumor agents were measured, results to simlar with the sensitivety in vivo were obtained.

体内扩散盒(Diffusion chamber)培养可使细胞在较接近自然的情况下生长。本文动态地观察了4株小鼠白血病(L615、L7212、L1210、P388)细胞在扩散盒中生长的过程。培养6~7天,细胞经对数生长期后可达生长高峰,细胞数可为植入时的5~14倍。如果使受鼠口服或肌注抗癌药物,可影响扩散盒内细胞的生长,其作用与肿瘤细胞对抗癌药物的敏感性有关。 本文研究了体内扩散盒培养的P388细胞对11种抗癌药物的敏感性,其中体内给药有效(生命延长率>25%)者7种,无效者4种。结果除6-巯基嘌呤的生命延长率为33%,而扩散盒培养细胞的生长抑制率为13.3%外(>35%为有效),其余均相符合。 本方法将抗癌药物筛选的体内、外实验法的优缺点结合起来考虑,国内外尚未见报道。它具有能综合反映药物对机体毒性、在体内代谢情况以及肿瘤细胞对药物敏感性的特点,并可用于培养人肿瘤细胞。对于寻找人肿瘤细胞敏感的抗癌药物,指导临床用药以及探索药物作用机理具有一定价值,从而为抗癌药物的筛选提供了一个新的途径。

After peritoneal injection of 2×108 syngeneic neonatal thymocytes mixed with neonatal donor heart cells, the mean survival time (MST) of allografted split hearts (C57BL-CFW) was obviously prolonged in the light irradiated (200 Rad 60Co irradiation from a back source) adult CFW host. The MST of allografts in neonatal thymocytes group is 20.0±1.8 days against 12.9 ± 1.5 days in control group and 16.2 ± 3.0 days in adult thymocytes group. The results show that neonatal thymocytes may exert an immunosuppres-sive...

After peritoneal injection of 2×108 syngeneic neonatal thymocytes mixed with neonatal donor heart cells, the mean survival time (MST) of allografted split hearts (C57BL-CFW) was obviously prolonged in the light irradiated (200 Rad 60Co irradiation from a back source) adult CFW host. The MST of allografts in neonatal thymocytes group is 20.0±1.8 days against 12.9 ± 1.5 days in control group and 16.2 ± 3.0 days in adult thymocytes group. The results show that neonatal thymocytes may exert an immunosuppres-sive effect on the allotransplantation rejection.The analysis of various agents given pre-transplantation shows that light irradiation did not affect the MST of allografts too much, if any, while the combination of irradiation and neonatal-donor heart cell injection do prolong MST to a certain degree. The host's immune function may recover in 5-7 days after such an irradiation treatment. Further investigation shows that the combination of irradiation and neonatal donor heart cell injection may be the cause of the prolongation of MST of allografts in adult mice thymocytes group.

经~(60)Co轻度照射(200Rad)之成年CFW小鼠,在接受2×10~8同品系新生早期胸腺细胞及供体新生心肌细胞混悬液腹腔注射后,其对同种半心移植(C57BL CFW)的排异反应明显延缓。新生鼠胸腺细胞组之移植物平均存活期(20.0±1.8天)比对照组(12.9±1.5天)和成年鼠胸腺细胞组(16.2±3.0天)显著延长。提示新生小鼠胸腺细胞对排异反应有抑制作用。 就各种术前处理对移植物存活期影响所作的分析表明,照射对移植物存活期无显著影响,在照射后的5—7天,受鼠免疫系统功能已回复正常水平。而照射联合新生供体心肌细胞注射,则在一定程度上延长移植物的存活期。进一步的分析显示,成年鼠胸腺细胞组移植物的存活期之所以较长,可能是由于受照射联合新生供体心肌细胞注射的影响。

The response of EAC-bearing host to thymus-dependent antigen (TDA) was assayed by plaque-forming cell (PFC) and hemagglutination (HA) test. It was found that the number of antibody-forming cell and titer of antibody to SRBC decreased with EAC development. Transfer of normal thymocytes and spleen cells could enhance the response of recipent to TDA, but not with the EAC-bearing donor lymphocytes. Slpecn cells or thymocytes plus normal bone marrow cells from EAC-bearing mice, contrary to normal donor, were inefficient...

The response of EAC-bearing host to thymus-dependent antigen (TDA) was assayed by plaque-forming cell (PFC) and hemagglutination (HA) test. It was found that the number of antibody-forming cell and titer of antibody to SRBC decreased with EAC development. Transfer of normal thymocytes and spleen cells could enhance the response of recipent to TDA, but not with the EAC-bearing donor lymphocytes. Slpecn cells or thymocytes plus normal bone marrow cells from EAC-bearing mice, contrary to normal donor, were inefficient in immunological reconstitution when they transplanted into lethally γ-rays irradiated recipents. The results suggested that deficiency of host response to TDA might be associated with decline of helper activity in thymus and spleen of tumor-bearing mice.

本文采用溶血空斑(PFC)和血凝(HA)试验探讨 Ehrlich 腹水癌(EAC)带瘤鼠在不同程度胸腺萎缩时对胸腺依赖性抗原(TDA)的反应性。发现接种 EAC后,抗体形成细胞数量和血清抗体效价逐渐下降;用带瘤鼠的胸腺和脾细胞转移不能促进受鼠对TDA的反应性,其胸腺细胞加骨髓细胞或脾细胞移植对致死性γ射线照射小鼠的免疫重建能力,明显低于正常对照组。结果表明:带瘤鼠在胸腺萎缩后,对TDA的反应能力明显下降,其机制可能与宿主胸腺和脾中的辅助细胞活性下降有关。

 
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