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重组杂交
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  recombinant hybrid
     A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10 ̄6u/mg protein and 1.4 × 10 ̄6u/mgprotein respectively.
     采用国产的微孔玻璃珠吸附层析法分离纯化人干扰素γ/肿瘤坏死因子β重组杂交蛋白,它具有抗病毒和抗肿瘤的双重功能活性,其抗病毒活性为1.7×10 ̄6u/mg蛋白,细胞毒活性为1.4×10 ̄6u/mg蛋白。
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  “重组杂交”译为未确定词的双语例句
     AIM To explore the amplification of cyclin D1 and its correlation to HBV infection and pathology during the development of human hepatocellular carcinoma (HCC). METHODS Forty specimens of fresh paired tumor and nontumor liver tissues from surgical resections were used for detection of genetic alterations of cyclin D1 , CD K 4 and HBV infection by the Southern hybridization. The samples were also examined histologically for pathological correlation with the genetic changes.
     目的探讨癌基因cyclinD1在肝癌发生发展中的作用以及与HBV感染和临床病理之间的关系.方法取手术切除的肝细胞癌标本,用Southern杂交的方法对cyclinD1和CDK4癌基因以及HBV的感染状态进行了检测,切片同时行病理检查.结果肝癌39例中9例cyclinD1存在2~30倍的扩增(237%),其中1例检出重组杂交带;
短句来源
  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     e-Hybrid Rice
     e杂交
短句来源
     The culture conditions of recombinant E.
     对重组E.
短句来源
     This recombinant plasmid pRLB1 was identified by digestion and Southern analysis.
     通过酶切、Southern杂交鉴定重组质粒.
短句来源
     northern hybridization.
     Northern杂交
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  recombinant hybrid
It was demonstrated that both the recombinant hybrid proteins and the native Gl7ACA acylase produced in E.
      
Recombinant hybrid genotypes are intermediate in fitness, with M.
      
Using the same amount of nutrients as in the batch fermentations, it was possible to increase the final biomass concentration to 20 g/l, plasmid-bearing cells in the population to 90% and recombinant hybrid protein to 1.21 g/l.
      
The disruption of those coadapted gene complexes would cause the lethality of the recombinant hybrid clones.
      
We used recombinant hybrid plants generated by crossing taxa in the Piriqueta caroliniana complex to assess the effects of individual leaf traits and trait plasticities on growth in a temporally variable environment.
      
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A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10 ̄6u/mg protein and 1.4 × 10 ̄6u/mgprotein respectively.The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than...

A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10 ̄6u/mg protein and 1.4 × 10 ̄6u/mgprotein respectively.The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than that of single IFNγor TNFβ.The anti-tumor mechanism of this hybrid protein had been investigated.

采用国产的微孔玻璃珠吸附层析法分离纯化人干扰素γ/肿瘤坏死因子β重组杂交蛋白,它具有抗病毒和抗肿瘤的双重功能活性,其抗病毒活性为1.7×10 ̄6u/mg蛋白,细胞毒活性为1.4×10 ̄6u/mg蛋白。该杂交蛋白在体外对人宫颈癌细胞株(ME180),人胃癌细胞株(7901)和人肺癌细胞株(G6)均有明显的杀伤作用,并都显著地高于单一的IFNγ或TNFβ对这些肿瘤细胞的杀伤。本文还对γTNFβ的抑瘤机理进行了初步探讨。

AIM To explore the amplification of cyclin D1 and its correlation to HBV infection and pathology during the development of human hepatocellular carcinoma (HCC). METHODS Forty specimens of fresh paired tumor and nontumor liver tissues from surgical resections were used for detection of genetic alterations of cyclin D1 , CD K 4 and HBV infection by the Southern hybridization. The samples were also examined histologically for pathological correlation with the genetic changes. RESULTS Two to thirty...

AIM To explore the amplification of cyclin D1 and its correlation to HBV infection and pathology during the development of human hepatocellular carcinoma (HCC). METHODS Forty specimens of fresh paired tumor and nontumor liver tissues from surgical resections were used for detection of genetic alterations of cyclin D1 , CD K 4 and HBV infection by the Southern hybridization. The samples were also examined histologically for pathological correlation with the genetic changes. RESULTS Two to thirty fold amplifications of cyclin D1 gene were detected in 9 of 39 HCC samples (23 7%) and a structural aberration of the gene was found in one of those samples with amplification. The amplifications of CD K 4 gene were found in 5 of 37 HCC cases. HBV DNA was detected in 37 of 39 HCC samples (94 9%) and the integration of HBV DNA in 29 of 39 HCC samples (74 4%). By correlation analysis, cyclin D1 amplification significantly correlated with amplification of CD K 4, the integration of HBV DNA, the differentiation degree and size of tumors. The correlation coefficients were 0 51, 0 31,0 39 and 0 55 respectively, P <0 05 for all. CONCLUSION The amplification of cyclin D1 is related to HBV DNA integration and plays an important role in the development and progression of HCC.

目的探讨癌基因cyclinD1在肝癌发生发展中的作用以及与HBV感染和临床病理之间的关系.方法取手术切除的肝细胞癌标本,用Southern杂交的方法对cyclinD1和CDK4癌基因以及HBV的感染状态进行了检测,切片同时行病理检查.结果肝癌39例中9例cyclinD1存在2~30倍的扩增(237%),其中1例检出重组杂交带;37例中5例(135%)有CDK4的扩增;HBVDNA在肝癌组织中检出的阳性率为947%,HBVDNA整合率为744%.相关分析发现,cyclinD1的扩增与CDK4扩增,HBVDNA整合,肿瘤的分化高低和肿块大小等之间有相关关系,相关系数r分别为051,031,039和055,全部P<005.结论cyclinD1的扩增与HBVDNA的整合密切相关,并在肝癌的恶化进展中起着重要作用.

In order to investigate the possible role of mesenchyme forkhead 1 (MFH 1) in osteogenesis and osteoblast differentiation, the gene recombination and hybridization methods are used to produce anti mouse MFH 1 monoclonal antibody. The expression of MFH 1 induced by bone morphogenetic protein 2 (BMP 2) in myoblasts C2C12 was examined by Western blot and Northern blot analysis. The alkaline phosphatase (ALP) activity and osteocalcin were used as the markers of the osteoblasts lineage, and also were measured....

In order to investigate the possible role of mesenchyme forkhead 1 (MFH 1) in osteogenesis and osteoblast differentiation, the gene recombination and hybridization methods are used to produce anti mouse MFH 1 monoclonal antibody. The expression of MFH 1 induced by bone morphogenetic protein 2 (BMP 2) in myoblasts C2C12 was examined by Western blot and Northern blot analysis. The alkaline phosphatase (ALP) activity and osteocalcin were used as the markers of the osteoblasts lineage, and also were measured. The results showed that the anti mouse MFH 1 monoclonal antibody was able to identify specifically the mouse MFH 1 protein which was expressed in human bladder carcinoma HTB 9 cell transfected with CX MFH 1 plasmid by Western blot analysis. The myoblasts C2C12 could express the endogenous MFH 1 protein in its nucleus. MFH 1 protein and MFH 1 mRNA both increased markedly in C2C12 cells after treatment with BMP 2; after lowering the endogenous MFH 1 level by stably transfecting C2C12 cells with antisense MFH 1 sequence, the alkaline phosphatase(ALP) activity and production of osteocalcin induced by BMP 2 were significantly lowered in antisense MFH 1 cell lines than in control cell lines. It can be concluded that the results suggest that the BMP 2 induced MFH 1 protein may play an essential role in regulating the osteoblastic differentiation of myoblasts C2C12.

为了研究中胚叶叉头 1(MFH 1)基因在骨骼形成和细胞分化中的作用 ,利用基因重组、杂交瘤技术制作MFH 1单克隆抗体 ,利用蛋白质印迹和RNA印迹分析观察了骨成形蛋白 2 (BMP 2 )诱导小鼠肌胚细胞C2C12表达MFH 1、产生碱性磷酸酶和骨钙蛋白 .小鼠肌胚细胞C2C12低水平地表达内源性MFH 1蛋白以及导入小鼠MFH 1cDNA的人膀胱癌细胞HTB9也表达小鼠MFH 1蛋白 ,这种蛋白质定位于细胞核中 .用BMP 2处理后 ,MFH 1蛋白和mRNA在C2C12细胞中的表达显著地增加 .用反义MFH 1序列转染小鼠肌胚细胞C2C12可降低内源性MFH 1水平 ,BMP 2不能诱导导入反义MFH 1序列的肌胚细胞C2C12产生MFH 1蛋白 ,也不能诱导碱性磷酸酶 (ALP)活性和骨钙蛋白量的增加 .结果表明 ,BMP 2诱导的MFH 1蛋白在调节肌胚细胞C2C12向成骨细胞分化方面起关键作用 .

 
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