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抑制杂交
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  suppression hybridization
     CHROMOSOME in Situ SUPPRESSION HYBRIDIZATIONAPPLIED TO THE STUDY OF ACUTE PROMYELOCYTIC LEUKEMIA
     染色体原位抑制杂交在急性早幼粒细胞白血病研究中的应用
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  “抑制杂交”译为未确定词的双语例句
     Construction of MPTP-induced differentially expressed gene librarys of C57BL mice brain by suppression subtractive hybridization
     利用消减抑制杂交技术构建MPTP诱导的C57BL小鼠全脑差异表达基因文库
短句来源
     By means of a suppression subtraction hy bridization (SSH) method the subtracted cDNA of 5 day-old adult worm of Trichinella spiralis was prepared using cDNA of the 5 day-ol d adult worm as Tester and cDNA of the muscle larvae + 3 day-old adult worm as Driver and ligated to pT-Adv vector and then was transformed into Esche richia coli TOP 10F.
     利用差减抑制杂交 (SSH)技术 ,以旋毛虫 5日龄成虫的cDNA为试验方 (tester) ,以肌幼虫 + 3日龄成虫的cDNA为驱动方 (driver) ,制备 5日龄成虫差减cDNA ,并与 pT Adv载体相连接 ,转入大肠埃希氏菌TOP 10F。
短句来源
     Suppression Subtractive Hybridiza tion and Its Application in Study of Tumors
     消减抑制杂交及其在肿瘤研究中的应用
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     And the difference may be the reason of the attenuation of A16R. Tester DNA is defined as that in which the differences are being sought relative to a driver DNA. In this study, B. anthracis A16 was taken as tester and A16R as driver.
     本实验以炭疽杆菌A16为tester,A16R为driver,进行消减抑制杂交,得到了炭疽杆菌A16与A16R消减PCR的混合物,与T载体相连接后,转入大肠杆菌,构建了消减文库。
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     The results show that chloroform-ethanol can reduce the foliar SOD activities of hybrid rice in part and Mn SOD exists in hybrid rice leaves probably and spraying Cu 2+ and Zn 2+ may promote the SOD activities during the earing period.
     试验结果表明 :氯仿 -乙醇能够抑制杂交水稻抽穗期SOD的部分活性 ,有可能在杂交水稻叶片中存在MnSOD ; 抽穗期叶片喷施Cu2 +、Zn2 +混合液可提高SOD活性
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  相似匹配句对
     Inhibition
     抑制
短句来源
     Suppression Subtractive Hybridization and It’s Application
     抑制消减杂交及其应用
短句来源
     Methods: Suppression subtractive hybridization (SSH).
     方法 :抑制消减杂交法 (SSH)。
短句来源
     inhibit the apoptosis;
     抑制细胞凋亡;
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     northern hybridization.
     Northern杂交
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  suppression hybridization
Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific
      
The differentially expressed gene clones between the two rape lines were isolated by subtractive suppression hybridization (SSH).
      
Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombi
      
Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics
      
Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus
      
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This work began with chromosome preparation and microdissection. Booster IRS-PCRwas developed to amplify DNA dissected form human chromosome 1. The accuracy of microdissection was demonstrated by chromosomal in situ suppression (CISS)-hybridization.The amplified DNA fragments were ligated to pUC 18 vector at Smal site and the averagesize of the inserts was about 450bp.

本实验从染色体标本制备开始,引进强化的IRS-PCR方法,成功地扩增了染色体切割片段,并用原位抑制杂交法验证切割及扩增结果。同时,对扩增出的DNA片段进行克隆和鉴定。

The results of morphology ,karyotype and chromosome in situ suppression (CISS) hybridizaion to diagnose the 42 patients with acute promyelocytic leukemia (APL,M3) were reported. The translocation t (15;17)was detected in 39 (93%) of 42 patients by standard G-banding method and CISS-hybridization analysis. It was found that 3 patients had a normal karyotype (NN) and they did not carry out the CISS hybridization amalysis, 22 patients had mixture of normal and abnormal mitosis (AN) and 17 patients had only abnormal...

The results of morphology ,karyotype and chromosome in situ suppression (CISS) hybridizaion to diagnose the 42 patients with acute promyelocytic leukemia (APL,M3) were reported. The translocation t (15;17)was detected in 39 (93%) of 42 patients by standard G-banding method and CISS-hybridization analysis. It was found that 3 patients had a normal karyotype (NN) and they did not carry out the CISS hybridization amalysis, 22 patients had mixture of normal and abnormal mitosis (AN) and 17 patients had only abnormal mitosis (AA). Two patients had translocation t(15;17) and additional chromosome abnormalities and one patients a variaty tanslocation t(5;15). In the present ,37 patients were diagnosed with FAB M3a and 5 patients with M3b. Among 5 patients with M3b,4 patients were mistaken for the other types of acute myeloid leukemia. After chromosome and CISS-hybridization analysis ,t( 15 ; 17) was detected and then they obtained a correct diagnosis. The clinical data of 21 patients wre evaluated. 19(95%) patients achieved a complete remission (CR) after treatment by all-trans rentinal acid (ATRA).Two patients with a very complex abnirmalities and a variaty translocation did not obtained CR and they die from AIDS and cerebral hemorrhage,The significance to detect the minimal residual disease (MRD) by combination of the cytogenetic with molecular methods were discussed.

对42例急性早幼粒白血病(APL,简称M3)患者在形态学检测的基础上,采用骨髓染色体G显带检测结果:t(15;17)检出率93%,12例同时进行了染色体原位抑制杂交(CISS)检测,结果:10例初诊患者t(15;17)的检出率100%,在42例中,NN型(中期分裂相皆正常)3例,AN型(正常和异常克隆嵌合体)22例,AA型(中期分裂相皆正常)17例,简单易位[只有t(15;17)]36例,复杂易位(同时有附加异常)2例,变异易位t(5;15)1例,FAB分型M3a 37例,M3b 5例.通过形态学、细胞遗传学和CISS(MCC)分析:形态学的确诊率88%,5例曾误诊为急性非淋巴细胞白血病(ANLL)的其他类型.另2例核型分析为NN型,后经染色体和染色体FISH分析确诊,5例误诊者4例为M3b(细颗粒型),MCC结合可明显提高确诊率,在临床资料可供评价的21例中19例达到完全缓解(CR),2例具有复杂异常核型和变异易位者未能取得缓解,分别进展为难治性白血病和呼吸窘迫综合征而死亡,核型与其他一些临床参数如:患者初诊时的白细胞数、初诊时DIC发生率、全反式维甲酸(ATRA)综合征的发生率和达到完全缓解的...

对42例急性早幼粒白血病(APL,简称M3)患者在形态学检测的基础上,采用骨髓染色体G显带检测结果:t(15;17)检出率93%,12例同时进行了染色体原位抑制杂交(CISS)检测,结果:10例初诊患者t(15;17)的检出率100%,在42例中,NN型(中期分裂相皆正常)3例,AN型(正常和异常克隆嵌合体)22例,AA型(中期分裂相皆正常)17例,简单易位[只有t(15;17)]36例,复杂易位(同时有附加异常)2例,变异易位t(5;15)1例,FAB分型M3a 37例,M3b 5例.通过形态学、细胞遗传学和CISS(MCC)分析:形态学的确诊率88%,5例曾误诊为急性非淋巴细胞白血病(ANLL)的其他类型.另2例核型分析为NN型,后经染色体和染色体FISH分析确诊,5例误诊者4例为M3b(细颗粒型),MCC结合可明显提高确诊率,在临床资料可供评价的21例中19例达到完全缓解(CR),2例具有复杂异常核型和变异易位者未能取得缓解,分别进展为难治性白血病和呼吸窘迫综合征而死亡,核型与其他一些临床参数如:患者初诊时的白细胞数、初诊时DIC发生率、全反式维甲酸(ATRA)综合征的发生率和达到完全缓解的时间等无相关性(p>0.05).

A study of chromosome 6,9,13,15 and Y specific library probes to identify the chromosome abnormalities was reported,The marker chromosome which derived from the chromosome 15,the chromosome translocation of t(2.9), and the very few mosaic 47,XXY cell line in 46,XX cell were exactly identified respectively in patients.

本文报告作者运用荧光标记的6号、9号、13号、15号、和Y染色体探针,对12名患者的G显带外周血、骨髓或胎儿羊水细胞染色体标本进行染色体原位抑制杂交的研究。

 
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