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该蛋白
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  the recombinant proteins
     By western-blot the recombinant proteins can be recognized by patient's positive serum while the negative serum cannot resulted in the same blot band.
     Western -blot证实该蛋白能被患者阳性血清所识别 ,用阴性血清则未出现相应印迹。
短句来源
     The recombinant proteins of C. parvum L-dsRNA gene were obtained successfully which would be used to study the function and characteristic of C. parvum LdsRNA.
     成功的在原核表达系统中表达了微小隐孢子虫病毒L-dsRNA基因蛋白,可用于进一步研究该蛋白的功能和特性。
短句来源
     The recombinant proteins were analyzed with SDS-PAGE and Western blot. Results The pET-invA recombinant for Salmonella was successfully constructed and the recombinant InvA protein was expressed in E. coli at a relatively high level.
     结果成功构建了沙门菌pET-invA大肠杆菌表达重组子,实现了该蛋白在大肠杆菌中的高效表达,分离纯化的表达产物纯度达到电泳纯。
短句来源
     Western blotting analysis showed that the recombinant proteins had immunoreactivity and proved that the deleting of transmembrane regions had no significant effect on the antigenicity of the recombinant proteins.
     经Westernblot分析,融合蛋白具有一定的免疫学活性,表明摘除跨膜区对该蛋白的抗原性影响不大。
短句来源
  “该蛋白”译为未确定词的双语例句
     only the 5th instar larvae all had the specific allergen of 30 kD. We purified the protein of 30 kD by ion exchange chromatography and gel isolation and identified it as ectoblast protein by MALDI-TOF-MS.
     但只有5龄家蚕的30kD蛋白为特异性变应原,通过离子交换层析和经切胶纯化出30kD蛋白,再经MALDI-TOF-MS鉴定该蛋白为外膜蛋白。
短句来源
     coli , which could induce splenocytes to produce high activity of IFN-γ and the highest was 1×106 U/mL produced by 250 ng/mL of recombinant chicken IL-18 protein, but could not directly inhibit the growth of VSV in CEF.
     该蛋白能够诱导脾细胞产生IFN-γ,当浓度为250ng/mL时诱导IFN-γ的活性最高,可达1×106U/mL; 但该蛋白不能直接抑制水疱性口炎病毒(VSV)在鸡胚成纤维细胞(CEF)上生长;
短句来源
     The recombinant LI protein was highly expressed at yield about 39.2% of total protein in Escherichia coli BL21(DE3)strain by 1.0 mmol/L IPTG induced and was only present in the inclusion body.
     其重组菌株在37℃条件下,经1.0mmol/L IPTG诱导表达重组LI蛋白,该蛋白以包涵体形式存在,表达量占菌体总蛋白的39.2%。
短句来源
     The reconstructed plasmid pET28a-fnbB was transformed into E. coli BL21(DE3)competent cell. The bacterium was induced by IPTG(1 mmol/L)and analyzed by SDS-PAGE,approximately 165 kDa exogenous protein was observed on the SDS-PAGE.
     coliBL21(DE3)感受态细胞中,经1mmol/L的IPTG诱导和SDS-PAGE分析,在约165ku处出现了与预期目的蛋白相一致的外源蛋白带,Western blot分析结果表明该蛋白具有金黄色葡萄球菌的抗原性。
短句来源
     Results:The purified protein with a band of molecular mass 44kDa were got in SDS-PAGE,and it bind specifically to mAbE4B7 in Western-blot and ELISA assay.
     结果:SDS-PAGE电泳表明纯化蛋白的相对分子量约为44kDa,Western-blot及ELISA鉴定显示该蛋白可与抗γ-精浆蛋白的单抗E4B7特异性结合。
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     Western blotting tested the expressed proteins.
     蛋白表达。
短句来源
     MLL protein;
     MLL蛋白;
短句来源
     TaLEA3 was mainly located in cytoplasm as revealed by the green fluorescent protein (GFP) fused to it.
     蛋白主要定位于细胞质。
短句来源
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  the recombinant proteins
tularensis was analyzed using specific antisera to the recombinant proteins Ag85-(His)6 and ESAT-6-(His)6 isolated from Escherichia coli producer strains created on the basis of the pET23b(+) and pET24b(+) vectors.
      
The recombinant proteins AglB and AglA were purified to homogeneity and characterized.
      
coli and purification of the recombinant proteins with a yield of about 10 mg per liter culture.
      
The recombinant proteins retained the capability of oligomerization, characteristic of their natural analogs.
      
The recombinant proteins were isolated from the culture medium by affinity chromatography.
      
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It was found that the muscle of an Ascidian (Ciona intestinalis Linne), an animal belonging to the subphylum Urochordata in the Phylum Chordata, contained a paramyosin component. Ethanol-dried muscle powder was extracted with a molar KC1 solution containing 5 mMEDTA and 5 mMTris-HCl, pH 7.5, followed by dialysis against water and 0.03 M-phosphate buffer (pH6.1)at 0~4℃. The paracrystals which formed were negatively stained with uranyl acetate and examined under the electron microscope. In addition to a periodicity...

It was found that the muscle of an Ascidian (Ciona intestinalis Linne), an animal belonging to the subphylum Urochordata in the Phylum Chordata, contained a paramyosin component. Ethanol-dried muscle powder was extracted with a molar KC1 solution containing 5 mMEDTA and 5 mMTris-HCl, pH 7.5, followed by dialysis against water and 0.03 M-phosphate buffer (pH6.1)at 0~4℃. The paracrystals which formed were negatively stained with uranyl acetate and examined under the electron microscope. In addition to a periodicity of 1, 500~1, 600A, which remained essentially unchanged through further crystallization, even in the presence of ammonium acetate at pH 8.0, there were also subspaoing of 900, 600, 150, 86 and 81 A.The solubility behavior and the stability towards ethanol treatment resemble those of a typical paramyosin. However, this Ascidian protein crystals showed a different cross-banding pattern as compared to that characteristic of the paramyosin of other sources. No serological cross-reaction was observed between this protein and amphioxus paramyosin.

脊索动物门尾索亚门的海鞘(Ciona intestinalis)肌肉中可抽提出副肌球蛋白,该蛋白对有机溶剂稳定并能在低离子强度环境形成纤维状晶体。海鞘副肌球蛋白纤维状晶体长度可达200微米,用电子显微镜观察其负染后的样品,具有横纹结构,横纹的总周期在150~160毫微米,其中包含有93与60毫微米的亚周期,另有若干细节在8.1与8.6毫微米左右。我们尚未见到海鞘和文昌鱼的副肌球蛋白之间有何免疫交叉反应。

It was found that the muscle of an Ascidian (Ciona intestinalis Linnè), an animalbelonging to the subphylum Urochordata in the Phylum Chordata, contained aparamyosin component. Ethanol-dried muscle powder was extracted with a molar KClsolution containing 5mMEDTA and 5mMTris-HCl, pH 7.5, followed by dialysisagainst water and 0.03 M-phosphate buffer (pH6.1)at 0~4℃. The paracrystalswhich formed were negatively stained with uranyl acetate and examined under theelectron microscope. In addition to a periodicity of...

It was found that the muscle of an Ascidian (Ciona intestinalis Linnè), an animalbelonging to the subphylum Urochordata in the Phylum Chordata, contained aparamyosin component. Ethanol-dried muscle powder was extracted with a molar KClsolution containing 5mMEDTA and 5mMTris-HCl, pH 7.5, followed by dialysisagainst water and 0.03 M-phosphate buffer (pH6.1)at 0~4℃. The paracrystalswhich formed were negatively stained with uranyl acetate and examined under theelectron microscope. In addition to a periodicity of 1,500~1,600(?), which remainedessentially unchanged through further crystallization, even in the presence ofammonium acetate at pH 8.0, there were also subspacing of 900, 600, 150, 86 and81(?).The solubility behavior and the stability towards ethanol treatment resemblethose of a typical paramyosin. However, this Ascidian protein crystals showed adifferent cross-banding pattern as compared to that characteristic of the paramyosinof other sources. No serological cross-reaction was observed between this protein andamphioxus paramyosin.

脊索动物门尾索亚门的海鞘(Ciona intestinalis)肌肉中可抽提出副肌球蛋白,该蛋白对有机溶剂稳定并能在低离子强度环境形成纤维状晶体。海鞘副肌球蛋白纤维状晶体长度可达200微米,用电子显微镜观察其负染后的样品,具有横纹结构,横纹的总周期在150~160毫微米,其中包含有93与60毫微米的亚周期,另有若干细节在8.1与8.6毫微米左右.我们尚未见到海鞘和文昌鱼的副肌球蛋白之间有何免疫交叉反应。

With the aid of cellulose chromatography and preparative electrophore-sis, a purified cofactor-deficient MoFe-protein has been obtained from A. V.mutant strain uw-45 with a yield of 18.4%.After reconstitution of the cofactor-deficient MoFe-protein with the FeMoco,a new active protein was formed with a molecular weight of 220,000.The active protein could reduces CH = CH to CH2=CH3 having a specific activity of 5.85nM/min · mg protein as much as 22.7 times of the extracts of A.V.mutant strain uw-45. Similar to...

With the aid of cellulose chromatography and preparative electrophore-sis, a purified cofactor-deficient MoFe-protein has been obtained from A. V.mutant strain uw-45 with a yield of 18.4%.After reconstitution of the cofactor-deficient MoFe-protein with the FeMoco,a new active protein was formed with a molecular weight of 220,000.The active protein could reduces CH = CH to CH2=CH3 having a specific activity of 5.85nM/min · mg protein as much as 22.7 times of the extracts of A.V.mutant strain uw-45. Similar to the MoFe-protein,the migration of the active protein during the course of electrophoresis was found to be slower than that of the cofactor-deficient MoFe-protein. Elemental analysis showed that the iron content was twice as much as that of the cofactor-deficient MoFe-protein. The visible absorption spectrum of the active protein was found to be identical with that of MoFe-protein. On the basis of the above results we postulate that the cofactor-deficient MoFe-protein can be transformed into MoFe-protein by combining with the FeMoco.

应用DEAE-11~#纤维素柱层析,结合无氧制备电泳从棕色固氮菌变种uw_(45)无细胞抽提液中分离得到了一种电泳纯蛋白质,回收率为18.4%。该蛋白与FeMo_(co)重组可以催化乙炔还原成乙烯,比活可达5.85nM乙烯/分·毫克蛋白,比其无细胞抽提液活性提高22.7倍。该蛋白与FeMo_(co)重组之后,电泳迁移率明显变慢,更接近棕色固氮菌钼铁蛋白,含铁量增加一倍,井含钼,分子量22万,可见光谱与棕色固氮菌钼铁蛋白的相同。因此,我们认为uw_(45)缺辅基铜铁蛋白与FeMo_(co)重组之后形成了类似棕色固氮菌钼铁蛋白的活性蛋白。

 
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