The structure and working principle of motion control system for a three-wheel mobile robot was introduced in this paper. A method of hierarchical controlling was used. AT89S52 was adopted as core-controller of on-board control system.
The single-stage paper-dry-air-filter of 3Y 12/15 tri-wheel roller is remoulded through making a double-stage air filtering system that adopts the upper parts of Dong Fang Hong 60T bulldozer air filter and 135 family wet-air-filter.
In RDA system, the tester amplicon is AKM46 while the driver amplicon from AGZ2. After three rounds of subtractive hybridization, six different products were obtained and identified, and their size was about 100bp~300bp.
Anti chlamydia monoclonal antibody C17 was used to biopan a 12mers phage display peptide library. After three rounds of biopanning and completing sequence analysis,a consensus sequence(L/I)PGGS(P/W) was obtained.
After three rounds biopanning, the positive clones were identificated by sandwich ELISA, competition ELISA and DNA sequencing. Amino acid sequences of 10 positive clones were compared with the JEV E protein.
方法 链亲和素包被塑料平皿 ,加入生物素标记 2 F2与噬菌体随机 15肽库 ,再洗脱 ,扩大培养进行三轮淘筛 ,经夹心 EL ISA、竞争 EL ISA鉴定后 ,挑取 10个阳性克隆 ,DNA测序 ,与 JEV E蛋白同源分析 .
After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M-1 were chosen.
The library was screened using thc BADH cDNA of mountiain spinach as a probe and six positive clones were obtained through three rounds of screening.
I presuppose that the group will want to have three rounds of discussion.
According to the size of the group these three rounds can take place in one long session - with the larger group breaking down in smaller groups and coming back together three times, for instance during an evening - or in three shorter sessions.
These recycle findings suggested that the enzyme remained relatively active for three rounds of recycle.