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斑点杂交结果
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  dot blot hybridization test
     The total RNA was 14 μg in the test group, while 22.5 μg in the control group. In both groups, 14 μg RNA was used for dot blot hybridization test with EGFcDNA probe.
     从对照组小梁细胞提取RNA 2 2 5 μg ,地塞米松组提取RNA 14μg ,取 14μg两组等量RNA ,用EGFcDNA探针进行斑点杂交 ,结果阳性。
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  “斑点杂交结果”译为未确定词的双语例句
     RESULTS: Titer of rAAV-CD151 was 2×1011 pfu/ml, titer of rAAV-antiCD151 was 1×1011 pfu/ml.
     结果:斑点杂交结果显示rAAV-CD151和rAAV-antiCD151病毒滴度分别为2.0×1011pfu/ml,1.0×1011pfu/ml;
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     RESULTS: HPV16 L1/L2/E6/E7 genes were integrated into vaccina virus DNA;
     结果经斑点杂交结果显示重组病毒基因组中有L1、L2、E6、E7基因插入;
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     Both u P Aand u P A R m R N A were expressed by the four celllines .
     斑点杂交结果示4 株细胞中均有u P A m R N A 和u P A R m R N A 的表达。
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     ②IL6 mRNA in human fetal islet exposed to rhTNFα is higher than control in dot hybridization;
     ②斑点杂交结果显示rhTNFα刺激的胰岛RNA中IL6mRNA含量高于对照组;
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     (3)IL-6 mRNA in human fetal islet exposed to rhIL-1β is higher than control in dot hybridization;
     (3)斑点杂交结果显示rhIL 1β刺激的胰岛RNA中IL 6mRNA含量升高 ;
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  相似匹配句对
     RNA dot hy- bridization.
     RNA斑点杂交
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     The results mentioned above are also proved by dot blot.
     同时斑点杂交结果也印证了上述结果
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     Results:1.The morphologic observation of HE coloration of cardiac muscle tissues.
     结果:
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     As a result, 2000 pcs.
     结果是;
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A DHBV preS/S expression plasmid(D F.W.29)was constructed with vector PEX34a. SDS-PAGE and laser scanning showed a unique band of fusion protein (MW 30000)accounting for 16% of total bacterial proteins.Antibody against the fusion protein was prepared by immunizing rabbits with the gel band and testified with Western blot analysis. The oc-currence of DHBsAg in duck serum samples was determined by Westera blot. Our data seem to be mostly consistent with those obtained by dot hybridization of DHBV-DNA. The antibody...

A DHBV preS/S expression plasmid(D F.W.29)was constructed with vector PEX34a. SDS-PAGE and laser scanning showed a unique band of fusion protein (MW 30000)accounting for 16% of total bacterial proteins.Antibody against the fusion protein was prepared by immunizing rabbits with the gel band and testified with Western blot analysis. The oc-currence of DHBsAg in duck serum samples was determined by Westera blot. Our data seem to be mostly consistent with those obtained by dot hybridization of DHBV-DNA. The antibody against the fusion protein therefore, can be usad to reveal DHBV carrier conditions and tin relationship between duck hepatitis B and liver cancer.

用重组DNA技术构建了鸭乙型肝炎病毒(DHBV)的Pre S/S的表达质粒——D.F.W.29,表达的融合蛋白分子量为30kd。用SDS-PAGE分离细菌蛋白,经考马斯亮蓝染色、激光光密度扫描定量分析表明,DHBV的pre S/S表达的融合蛋白占细菌总蛋白的16字。将此融合蛋白免疫家兔,可获得相应抗体。免疫印迹法(Western blot)测抗体灵敏度,证明和该抗体呈现阳性反应的最低抗原量为3μg。用此抗体检测鸭血清中DHBsAg的初步结果表明,它与鸭血清DHBV DNA斑点杂交结果基本相符。因此,该抗体可以用作检测鸭乙型肝炎病毒携带状态并可用于研究鸭肝癌与肝炎的关系。

The c-myc, Ha-ras, v-sis and v-erbB oncogenes are used as proles to detect the HindⅢ, EcoR Ⅰ or BamHI digested brain DNA of 2 cases of normal brain and 8 cases of brain tumors (2 astrocytomas, 2 meningiomas, 1 ependymoma and 2 glioblastoma multiform) by Southern blot analysis. The results show that there is a 10.0kb (HindⅢ) c-myc gene band in both normal and malignant brain DNA samples. In one case of ependymoma (HG-2) and one case of meningi-oma (HG-3), an extra 7.0kb (HindⅢ) c-myc gene band is found. The dot...

The c-myc, Ha-ras, v-sis and v-erbB oncogenes are used as proles to detect the HindⅢ, EcoR Ⅰ or BamHI digested brain DNA of 2 cases of normal brain and 8 cases of brain tumors (2 astrocytomas, 2 meningiomas, 1 ependymoma and 2 glioblastoma multiform) by Southern blot analysis. The results show that there is a 10.0kb (HindⅢ) c-myc gene band in both normal and malignant brain DNA samples. In one case of ependymoma (HG-2) and one case of meningi-oma (HG-3), an extra 7.0kb (HindⅢ) c-myc gene band is found. The dot blots show an amplification of c-myc gene (2-8 folds) in HG-2 and HG-3. In addition to the rearrangement and amplification of c-myc gene, an amplification of erbB gene (8 folds) is also observed in HG-2 and HG-3. With Ha-ras gene probe, anormal 6.6kb (BamH Ⅰ) band is observed in each brain sample, but no aberrantband. There is no v-sis gene band in all of the brain DNA samples digested byBamH Ⅰ .The results of this study suggest that activation of c-myc gene may be involved in the occurrence of ependymoma and meningioma, and also demonstrate that more than one oncogenes are involved in carcinogement.

用c-myc、Ha-ras、v-sis及v-erbB四种癌基因为分子探针,对8例人脑原发性肿瘤和两例正常人脑DNA进行Southern吸印杂交分析。结果显示:以c-myc基因为探针进行分子杂交时,所有被检测肿瘤和正常对照都可见一条相同的10.0kb(HindⅢ酶切)人c-myc基因区带。其中一例室管膜瘤和一例脑膜瘤还出现另一条异常的7.0kb(HindⅢ酶切)c-myc基因区带。斑点杂交结果表明,上述两例肿瘤中有c-myc基因的扩增(2—8倍),同时还具有erbB基因的扩增(8倍)。Ha-ras及v-sis基因杂交,未见异常区带。上述结果提示室管膜瘤和脑膜瘤的发生可能与c-myc基因的激活有关;c-myc基因的异常变化与erbB基因扩增同时出现,可能为癌基因之间的协同作用提供证据。

The expression of 5 oncogenes was examined in 4 developmental stages (embryo, newborn, sexual maturity and adult)of CWE mouse brain tissue.Brain RNA was detected with Ha-ras, c-fos, c-myc v-erb B and mos oncogene probes by dot blot hybridization. The expression of these oncogenes was demonstrated.Ha-ras, c fos, c-myc were expressed at high level in the embryo and newborn brain, but erbB showed higher expression at the stage of sexual maturity. In contrast, mos oncogene showed no expression at all.At the same...

The expression of 5 oncogenes was examined in 4 developmental stages (embryo, newborn, sexual maturity and adult)of CWE mouse brain tissue.Brain RNA was detected with Ha-ras, c-fos, c-myc v-erb B and mos oncogene probes by dot blot hybridization. The expression of these oncogenes was demonstrated.Ha-ras, c fos, c-myc were expressed at high level in the embryo and newborn brain, but erbB showed higher expression at the stage of sexual maturity. In contrast, mos oncogene showed no expression at all.At the same time the DNA of brain tissue was studied by dotblot hybridization, the result showed that there was no oncogene amplification in any developmental stage; by Southern blot transfer of DNA digested with BamH I, 4 c-fos oncogene bands of 5.4, 3.4, 1.8, 1.0kb and 3 c-myc oncogene bands of 7.4, 4.3, 3.4kb were observed, but there was no difference in these 4 stages.

本文以Ha-ras.c-fos、c-myc、v-erbB、mos五种癌基因片段为分子探针,应用斑点杂交技术,对CWE纯系小鼠脑组织发育的四个时期(胚胎期、新生期、性成熟期、体成熟期)的RNA进行分子杂交分析。发现:在实验各期中这几种癌基因的表达有较明显的变化。Ha-ras、c-fos、c-myc基因的表达在胚胎期和新生期脑组织中较高;v-erbB基因的表达在性成熟期脑组织中较高;mos基因在实验各期均无表达。DNA斑点杂交结果:小鼠脑组织发育各期均未观察到癌基因扩增现象。Southern杂交结果:CWE小鼠发育的各期均显示:5.4kb、3.4kb、1.8kb、1.0kb四条c-fos基因区带;7.4kb、4.5kb、3.4kb三条c-myc基因区带。结果表明:不同细胞癌基因在小鼠胚胎发育和胚后发育中有不同的表达。

 
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