Lymphocytes taking from 6 healthy individuals were divided into 6 groups and irradiated by￣(60)Coγ-rayin 0,6,10,15,20,and 25Gy respectively, then the mixed lymphocyte culture was performed.
Methods Mixed lymphocyte culture reaction was used as the experimental model. The expressions levels of IFN-γ and TNF-α mRNA were measured by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
After the mitomycin C treated HC were cocultured with the spleen cells of BAC mouse (H 2 dxb ) as a one way mixed lymphocyte culture (OWMLC ) for 6 days, the mixed cell were labeled by Annexin V/PI kit and the apoptosis rate was assayed by FCM.
GL-A (16-250 μg·ml~(-1)), GL-B (62-250 μg·ml~(-1)) andGL-C (16-250 μg·ml~(-1)) markedly increased the rate of [~3H] TdR uptake by spleen cells inmixed lymphocyte culture (MLC) and the effects were positively correlated with concentrationsof the drugs.
The changes of MLC and serological HLA antibodies in the recipients were observed before blood transfusions (T0), one month (T1), two months (T2) and three months (T3) after transfusions, respectively.
Results p140 molecule is expressed selectively on the activated T cells generated from MLC,whereas PBMCs activated by anti CD3 mAb,PHA,PMA or PMA plus A23187 didn't express this membrane molecule.
Koumine (Kou) inhibited, to some extent, mixed lymphocyte response and concanavalin A (2μg/ml) or lipopolysaccharide (10μg/ml) induced proliferation of murine splenocytes in vitro and the minimum concentrations were 10, 5 and 40μg/ml respectively.
Methods An one-way mixed lymphocyte reaction system characterized by labeling responder cells with CFSE and stimulator cells with SNARF-1 was established to measure the alloresponse mediated by syngeneic(BALB/c×BALB/c) and allogeneic(BALB/c×C57) pregnant mice,called syn-BALB/c and allo-BALB/c respectively,to C57 alloantigens at 10.5 days post coitus.
The mixed lymphocyte culture (MLC) is an established clinical method for bone marrow transplantation, as it serves as an in vitro model for allogenic reaction and transplantation.
The level of CD8+ cells was and remained within the normal range, although suppressive activity generated in mixed lymphocyte culture was deficient prior to therapy.
For example, they proliferated in response to allogeneic mixed lymphocyte culture (MLC); in addition, MLC-derived TCR gamma/delta+ cells specifically lysed PHA-induced blast cells bearing the stimulating alloantigens.
Purified human anti-hIL-2 antibodies can interfere with lymphocyte proliferation both in the lymphokine activated killer cell assay and in the mixed lymphocyte culture.
Instead, they can inhibit the expression of HLA-DR antigens induced by IFN-γ on U937 cells and interfere, in mixed lymphocyte culture, with the proliferation of lymphocytes and the generation of cytotoxic lymphocytes.
Synthesis of the main contractile proteins, primarily the components of myosin molecule-heavy chain (MHC) and individual isoforms of light chains (MLC1, MLC2, and MLC3)-are encoded by different genes during different ontogenetic stages.
The definitive fast muscle contained three light chain types, MLC1, MLC2, and MLC3; slow muscle, MLC1 and MLC3; while the larval muscle fibers included a specific larval MLCL in addition to MLC3.
mRNAs of all three types of myosin light chain proteins are expressed in skeletal muscles of both larval and adult stages of loach Misgurnus fossilis (Cobitidae) and these proteins are encoded by different genes (mlc1, mlc2, and mlc3).
Mixed lymphocytes cultures (MLC) and mixed skin epidermal cells-lymphocytescultures (MSLC) were done in 8 pairs and mixed fibroblasts and lymphocytes cultures(MFLC) in 4 pairs of non-related individuals.By quantitative determinations,the valueof ~3H-TdR incorporation of MSLC far exceeded that of the MLC,the average stimula-tion index of MSLC was 6.9 times that of MLC.All the MFLC revealed negativeresponse.The data indicate the extremely strong antigenicity of the skin epidermal cells,but on the contrary,the...
It has been shown that cell-free liver-extract from embryonic, newborn and adult mice livers contains a factor which can suppress in vitro the proliferative response of splenic and lymph node lymphocytes to mitogens (phytohemagglutinin, PHA; concanavalin A, Con A; lipopolysaccharide, LPS) and allogeneio antigens. The immunosuppressive activity of this factor was nonstrain-specific since the sup-pressive action on the response of lymphocytes from the donor mice and other strains as well was effective. Eesult...
The efficacy of different inducers used to induce immune interferon (IFN-γ)from ionsillar cells of onsillectomized normal children was sfudied.Tonsillar cells were made in suspension of 1×107 cells/ml treated with antibiotics,and cultured for 2-3 days and then supernants were assayed for IFN-r.The results show that cells of individual human tonsil could produce IFN-γ spo ntaneously owing to the induction by pharyngeal normal flora.Esculentoside (Es) alone or .pokeweed mitogen (PWM) alone,their combinatian o...
混合淋巴细胞培养
mixed lymphocyte culture
查询“混合淋巴细胞培养”译词为用户自定义的双语例句
相关查询:
2008 CNKI－中国知网