The DNA were extracted from buffy coat with phenol chloroform. Cytochrome P450(CYP)2E1 genotype were analyzed with restriction enzyme DraI for the sixth intron and Rsa I for the 5' flanking region.
1. PCR-RFLP analysis was used to detect the variations within intron 6 of the porcine NRAMP1 gene on 165 Large White (LW) pigs and 109 Zhongxu Black (ZB) pigs population which were reared in the same conditions.
Genotype and Allele Frequency of the Variable Number Tandem Repeat Polymorphism in the VNTR6-1st of the of Human Telomerase Reverse Transcriptase (hTERT) Gene in Chinese Population
In contrast with KM mouse, bysystematic studies on phenotype, histopathology, ultrastructure, intron 6 of hairless gene, cDNA cloning,gene sequencing and functional analysis, we identified and reported a novel hairless mutant gene and fivepolymorphisms in hairless gene.
Examination of the S3 chromatin structure in thymocytes, a dynamic population that undergo substantial proliferation, apoptosis, and differentiation, revealed an additional DNase I HS site mapping to the sixth intron of the genomic sequence.
Analysis of polymerase chain reaction amplified products from the sixth intron of the human interleukin-1α gene reveals a high polymorphism (polymorphism information content = 0.51) in a Caucasian population.