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表达载体     
相关语句
  expression vector
     The Studies on Constructing Expression Vector of Nematode Resistance Gene and Genetic Transformation
     抗线虫基因表达载体质粒构建和遗传转化研究
短句来源
     The Construction of Expression Vector of Fas/RAR Alpha Fusion Gene Containing Retinoic Acid Response Elemennt, and Its Effect Induced by Retinoic Acid on the Apoptosis of Tumor Cells
     视黄酸依赖Fas/RARα融合基因表达载体构建及其诱导肿瘤细胞凋亡的研究
短句来源
     The Study on High-level Expression of Hepatitis B Virus Surface Antigen (PreS2+S) and Immunnogenicity of Products Expressed in Silkworm/BmNPV Expression Vector System
     家蚕/BmNPV表达载体系统高效表达人乙肝表面抗原(PreS2+S)及表达产物免疫原性的研究
短句来源
     Construction of a Light Inducible Expression Vector and Its Application in Transformation of Dunaliella Bardawil
     光诱导表达载体的构建及其在盐藻除草剂抗性转化中的应用
短句来源
     Construction of Prokaryotic Expression Vector of MTB lhp-esat6 Fusion Gene and Preliminary Application of Recombinant CFP10-ESAT6 Fusion Protein
     结核分枝杆菌lhp-esat6融合基因原核表达载体的构建及重组CFP10-ESAT6融合蛋白的初步应用
短句来源
更多       
  expression vectors
     The Studies of the Construction of Specific Expression Vectors of Human DAF, CD59 Gene and Their Expression in Pig Endothelial Cells
     人DAF、CD59基因特异表达载体的构建及其在猪血管内皮细胞表达的研究
短句来源
     The effects of antisense oligonucleotide for c-myc, antisense eukaryotic expression vectors for c-myc and antisense recombinant adenoviral vector for c-myc on the proliferation of rat airway smooth muscle cells
     反义c-myc寡核苷酸、反义c-myc真核表达载体和反义c-myc重组腺病毒载体在大鼠气道平滑肌细胞增殖中的作用
短句来源
     Construction of adenovirus expression vectors of HCV structural proteins and study of T-cell vacclnes on clearance of HCV
     HCV结构蛋白腺病毒表达载体的构建及T细胞疫苗对HCV清除作用的研究
短句来源
     Construction of Highly Efficient Expression Vectors of HMW-GS 1By15 and 1Dx1.5~t Genes for Transformation of Wheat
     小麦(Triticum aestivum L.)高分子量麦谷蛋白1By15和1Dx1.5~t基因高效表达载体的构建及转化研究
短句来源
     Effects of Antisense EGF and Antisense EGFR Retroviral Expression Vectors on the Cell Growth of Human Pancreatic Cell Line
     人反义EGF和反义EGFR逆转录病毒表达载体对人胰腺癌细胞系细胞生长的影响
短句来源
更多       
  expression plasmid
     Objective To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin-neuraminidase(HN)of newcastle disease virus(NDV)oncolytic strain Italien,and then to express the protein in eukaryotic cell.
     目的构建天然溶瘤型新城疫病毒(NDV)Italien株血凝素-神经氨酸酶(HN)基因的真核表达载体并进行产物表达。
短句来源
     The secreted expression plasmid pPIC9K-man1 of Pichia pastoris was constructed and digested with SacⅠand transformed into Pichia pastoris GS115 by PEG (polyethylene glycol). PCR and phenotype analysis showed man1 has been integrated into P.pastoris genome.
     构建了重组表达载体pPIC9K-man1,重组质粒SacⅠ线性化后用PEG(聚乙二醇)法导入毕赤酵母(Pichia pastoris)菌株GS115中,通过PCR和表型鉴定表明,man1基因已经整合到毕赤酵母染色体上。
短句来源
     Methods The heparinaseⅡ gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum, and it was linked into expression plasmid pET-28a after sequencing, then the recombined plasmid was expressed in E.
     方法通过PCR从黄杆菌基因组DNA中扩增出黄杆菌HepⅡ的基因,经验证后插入到表达质粒pET-28a上构建表达载体,重组质粒转化E.
短句来源
     By using pEGFP-N_3 vector,the MIF-GFP expression plasmid,pEGFP-MIF,was constructed with the same Kozak consensus translation initiation site and start code ATG for MIF-EGFP coding sequence.
     构建拥有同一Kozak共有翻译启始序列、翻译启始密码子ATG的MIF-GFP融合表达载体pEGFP-MIF.
短句来源
     The 3 candidate MIF siRNA expression plasmids were also co-transfected with MIF expression plasmid into HEK293 cells, respectively, and the MIF mRNA expressions were determined by real-time quantitative PCR.
     同时,将MIFsiRNA表达质粒分别与MIF表达载体共转化HEK293细胞,用荧光定量PCR检测HEK293细胞中MIF mRNA的表达水平.
短句来源
更多       
  expressing vector
     To construct double expressing vector pLXSN-mB7-1-IRES-mB7-2 with murine B7-1 and B7-2 gene and package them into PA317 cell.
     目的构建含小鼠B7-1和B7-2基因的双表达载体pLXSN-mB7-1-IRES-mB7-2,并包装到PA317细胞中。
短句来源
     2. Fusion expressing vector of pGEX-4T-1/ TRP-1 was successfully constructed;
     2.成功构建了GST融合表达载体pGEX-4T-1/TRP-1;
     ②Fusion expressing vector of pGEX 4T 1/TRP 1 was successfully constructed;
     ②成功构建了GST融合表达载体 pGEX 4T 1/TRP 1;
短句来源
     Vector was constructed successfully which was proved by cutting, PCR and sequencing, and packaged into PA317. Double expressing vector pLXSN-mB7-1-IRES-mB7-2 and PA317/mB7-1-mB7-2 cells were obtained successfully.
     结论成功得到了双表达载体pLXSN-mB7-1-IRES-mB7-2和包装后的PA317/mB7-1-mB7-2细胞。
短句来源
     Construction and Identification of Eukaryotic Expressing Vector of pcDNA3.1(+)/NspA
     pcDNA3.1(+)/NspA真核表达载体的构建及鉴定
短句来源
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  expression vector
The cDNA fragment was inserted into the expression vector pET-28a, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3) by isopropylthiogalactoside induction.
      
In this study, XooR was expressed as a fusion protein along with green fluorescent protein (GFP), using pET-24a-d (+), a prokaryotic expression vector.
      
The genes xyl1 and xyl2 were placed under the control of promoter GAL in vector pYES2 to construct the recombinant expression vector pYES2-P12.
      
The murine IL-23 cDNA was sub-cloned into a dual-expression vector.
      
The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZα-B and transformed into P.
      
更多          
  expression vectors
Sense and antisense expression vectors of PtAP3 were constructed by PCR and restriction enzymes digestion identification, and transformed into tobacco (Nicotiana tabacum) by Agrobacterium GV3101 and LBA4404.
      
Several constructs of the expression vectors pKK OmpA and pET-3A with or without bacterial, leech, or yeast signal peptides (SP) were used.
      
Eukaryotic expression vectors and immunoconjugates for cancer therapy
      
These include transcriptional activation of tissue-and tumor-specific promoters in eukaryotic expression vectors and use of antitumor-lirected immunoconjugates.
      
Cotransfection of vectors containing these sequences linked to a reporter with p53 expression vectors resulted in stimulation of transcription.
      
更多          
  expression plasmid
The expression plasmid were constructed by inserting T800/T1300 into plasmid pET-28a/c respectively and were transformed into Escherichia coli BL21 (DE3).
      
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
      
The rCR gene could express in recombinant strain Escherichia coli JM109, and the expression plasmid could produce (R)-1-pheny-1,2-ethanediol (100% e.e., 80.14% yield) from β-hydroxyacetophenone without any additive to regenerate NAD+ from NADH.
      
In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins.
      
The gene of human interleukin-6 (hIL-6) with an additional 20 amino acids on the N-end, including six histidine residues, was cloned into the expression plasmid pET28b(+).
      
更多          
  expressing vector
coli BL21 (DE3) using pET-32a (+) as expressing vector.
      
Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis
      
We transfected Sf9 cells with an expressing vector p35IE1Neo containing antiapoptotic p35 gene and neomycin-resistant gene (as a selection marker).
      
Eukaryotic expressing vector containing transforming growth factor beta 1 (pcDNA3-TGFβ1) was encapsulated by the system.
      
Construction of eukaryotic expressing vector pCH503 of CH50 and its chemotaxis and anti-tumor function by expressionin vivo in m
      
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