The effects of antisense oligonucleotide for c-myc, antisense eukaryotic expression vectors for c-myc and antisense recombinant adenoviral vector for c-myc on the proliferation of rat airway smooth muscle cells
Objective To construct an eukaryotic expression plasmid containing gene coding for the hemagglutinin-neuraminidase(HN)of newcastle disease virus(NDV)oncolytic strain Italien,and then to express the protein in eukaryotic cell.
The secreted expression plasmid pPIC9K-man1 of Pichia pastoris was constructed and digested with SacⅠand transformed into Pichia pastoris GS115 by PEG (polyethylene glycol). PCR and phenotype analysis showed man1 has been integrated into P.pastoris genome.
Methods The heparinaseⅡ gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum, and it was linked into expression plasmid pET-28a after sequencing, then the recombined plasmid was expressed in E.
The 3 candidate MIF siRNA expression plasmids were also co-transfected with MIF expression plasmid into HEK293 cells, respectively, and the MIF mRNA expressions were determined by real-time quantitative PCR.
Vector was constructed successfully which was proved by cutting, PCR and sequencing, and packaged into PA317. Double expressing vector pLXSN-mB7-1-IRES-mB7-2 and PA317/mB7-1-mB7-2 cells were obtained successfully.
Sense and antisense expression vectors of PtAP3 were constructed by PCR and restriction enzymes digestion identification, and transformed into tobacco (Nicotiana tabacum) by Agrobacterium GV3101 and LBA4404.
Several constructs of the expression vectors pKK OmpA and pET-3A with or without bacterial, leech, or yeast signal peptides (SP) were used.
Eukaryotic expression vectors and immunoconjugates for cancer therapy
These include transcriptional activation of tissue-and tumor-specific promoters in eukaryotic expression vectors and use of antitumor-lirected immunoconjugates.
Cotransfection of vectors containing these sequences linked to a reporter with p53 expression vectors resulted in stimulation of transcription.
The expression plasmid were constructed by inserting T800/T1300 into plasmid pET-28a/c respectively and were transformed into Escherichia coli BL21 (DE3).
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
The rCR gene could express in recombinant strain Escherichia coli JM109, and the expression plasmid could produce (R)-1-pheny-1,2-ethanediol (100% e.e., 80.14% yield) from β-hydroxyacetophenone without any additive to regenerate NAD+ from NADH.
In this study, we have cloned the Chlamydia trachomatis genes incB and incC into the expression plasmid vectors from pET series for the subsequent isolation of recombinant proteins.
The gene of human interleukin-6 (hIL-6) with an additional 20 amino acids on the N-end, including six histidine residues, was cloned into the expression plasmid pET28b(+).