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发酵条件研究    
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The strain of Pseudomonas SIPI-215 has been screened out from371 strains with a rapid plate method,and its cultural conditions were iuvestiga-ted.The purity and thermostability of this esterase as well as the quality of theenzyme kit prepared with it were better than that formulated with the cholesterolesterase from bovine pancrease.

通过平板初筛和摇瓶复筛,从371株菌中筛得一株有工业生产价值的胆固醇酯酶产生菌,并对它进行了发酵条件的研究。用所获得的胆固醇酯酶配制的三酶试剂具有良好的稳定性,适用于临床检测。

The variant K211 strain was selected and bred from B.Subtilis BF-7658.The fermentatioa condiiion under shaking flask was investigated. The result showed that the optimumconsistency of culture media was 15-19%, the best ratio of carbon source to nitrogen source was two to one. and the activity of produced a-amylase was at 527u/ml. Further tests made in 23m3 fermentation tank showed that above consistency was suitable- the activity of produceda-amylase was at 460u/ml, which was the average value based on twelve...

The variant K211 strain was selected and bred from B.Subtilis BF-7658.The fermentatioa condiiion under shaking flask was investigated. The result showed that the optimumconsistency of culture media was 15-19%, the best ratio of carbon source to nitrogen source was two to one. and the activity of produced a-amylase was at 527u/ml. Further tests made in 23m3 fermentation tank showed that above consistency was suitable- the activity of produceda-amylase was at 460u/ml, which was the average value based on twelve batches. This fermentation process has the advantage of steady operation, and shorter fernemtatjoaperiod, so that better utilization oi equipment could be achieved.

对所选育出的枯草杆菌BF—7658变异株K211菌种,在摇瓶条件下进行了发酵条件的研究。研究结果表明菌种的发酵培养料浓度在15—19%之间,碳氮比以2/1为宜。在此浓度范围内,摇瓶发酵酶活力达527U/ml。经23M~3发酵罐放大试验证实,该配方是适宜的。在23M~3罐上发酵12罐,α—淀粉酶活力平均在460u/ml。用K211菌种生产α—淀粉酶具有生产性能稳定,均35小时)可提高设备利用率等优点。 提高α—淀粉酶生产菌种的产酶活力,是我国目前酶制剂行业中关注的问题之一。为达到提高α—淀粉酶活力的目的,我们对菌种和工艺条件进行了研究。有关枯草杆菌BF—7658变异株K211菌株的选育过程,在1986年第6期《食品与发酵工业》中已作报道。本文主要进行K211菌发酵工艺条件的研究。

This paper presents of studies on conditions for Nocardia sp. 61 fermentation cleavage of cholesterol to produce androsta-1, 4-diene-3, 17-dione (ADD).Optimal studies were undertaken for factors such as: Medium composition, alcohol concentration, pH, capacity of air input, effects of alcohol and substrate induce etc.The investigative efforts resulted in a 35% increase in ADD molar conversion yield under a 1‰ substrate concentration etc.

对诺卡菌SP·61降解胆固醇边链至雄甾1.4-二烯3.17-二酮(ADD)的发酵条件研究,可以选择最佳的发酵条件,提高ADD的转化率。本文就部分培养基成分的改变、通气量、pH、乙醇以及底物诱导等因素进行研究,从而使ADD的克分子转化率提高到35%(底物浓度为1%,转化时间为84h)。

 
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