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发酵条件研究
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  study on fermentation conditions
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Thrombolysis enzyme produced by Rhizopus sp. was able to dissolve fibrin but not lyse haemocyte. The enzyme acitvity was the highest when Rhizopus sp 12# was aerated in the medium containing bran and peptone (pH 5.1) at 30℃ for 48-60hr, and the end pH value is about 7.4.

通过对根霉12号发酵液的分析及发酵条件的研究,发现其发酵液中含有能溶解血栓的物质,但不能分解血细胞。它在麸皮胰蛋白胨培养基(pH5.1)上30℃振荡培养48~60h,pH值达到7.4左右时产生血栓溶解酶的活力最高。

The hexanoic acid generated from hexanoic acid bacterium is the essential component ofthe basic frangrance of spirits. lt can improve the frangrance and quality of spirits if it is ap-plied to production of spirits.A strain high-yielded hexanoic acid had been separated from cellar mud of FuYu brew-ery of HeiLongjiang province. It had been found that the highest output of the hexanoic acidcan reach 54omg/1O0ml by the research on fermentation conditions of this strain.

己酸菌所生成的己酸是生产浓香型白酒主体香气己酸乙酯的主要成分,将其应用于白酒的生产,可提高白酒的香气和质量.从我省富裕酒厂的窖泥中分离到一株高产己酸的菌株,通过发酵条件的研究,该菌株己酸最高产量可达540mg/100ml。

The culture and expression coditions of secretory recombinant E. coli W3100/pGM-CSF were optimized during the pilot fermention process. More than 25 % (about 1. 5g/L) of total somatic proteins of secrtory GM-CSF were expressed in W3100 culture in 30L fermenter under the improved conditions. The specific activities of product reached over 2.0×107/ mg protein after being purified,

通过对GM-CSF工程菌W3100发酵条件的研究,优化了培养及表达条件,使工程菌W3100在30L发酵罐可表达分泌型GM-CSF1.5g/L左右,占菌体总蛋白的25%以上,纯化后的比活大于2.0×107。

 
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