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发酵条件研究    
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  study on fermentation conditions
    STUDY ON FERMENTATION CONDITIONS OF Cephalosporium dongchongxiacae MYCELIUM
    冬虫夏草头孢菌菌丝体液体发酵条件研究
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    Study on Fermentation Conditions Involved in Chitinase Production of Marine Vibro Strain Z010
    海洋弧菌Z010 产生几丁质酶的发酵条件研究
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    Study on Fermentation Conditions of Vinegar with cutting Fat down and Analysis of Active Components
    降脂醋发酵条件研究及活性成分分析
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    Study on Fermentation Conditions of Fibrinolytic Enzyme by Bacillus subtilis HGD107
    豆豉纤溶酶产生菌发酵条件研究
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    Study on Fermentation Conditions in Laccase Synthesis by Neurospora crassa Mutant Strain
    粗糙脉孢菌突变菌株合成漆酶的发酵条件研究
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  studies on fermentation conditions
    STUDIES ON FERMENTATION CONDITIONS OF LACTOCOCCUS LACTIS AL2 WITH HIGH YIELD OF NISIN
    乳链菌肽高产菌株AL2的发酵条件研究
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    Studies on Fermentation Conditions for Key Enzymes in1,3-Propanediol Production with Klebsiella Pneumoniae
    克雷伯杆菌生产1,3-丙二醇关键酶发酵条件研究
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    Studies on fermentation conditions of α-chloropropionic acid dehalogenases
    α-氯丙酸脱卤酶发酵条件研究
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    Breeding of Valine-Producing Strain by Protoplast Fusion and Studies on Fermentation Conditions
    缬氨酸生产菌的原生质体融合育种及发酵条件研究
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    STUDIES ON FERMENTATION CONDITIONS FOR LACCASE PRODUCTION FROM CORIOLUS VERSICOLOR
    杂色云芝产漆酶的发酵条件研究
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  study on fermentation condition
    Study on Fermentation Condition of β-galactosidase by Bacillus sp.
    嗜热芽孢杆菌β-半乳糖苷酶发酵条件研究
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    STUDY ON FERMENTATION CONDITION FOR PCA PRODUCT BY Pseudomonas fluorescens M18
    荧光假单胞菌株M18产PCA发酵条件研究
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    Study on Fermentation Condition of Antagonistic Actinomycetes XA-1
    颉颃放线菌XA-1菌株发酵条件研究
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  studies on fermentation conditions
Studies on fermentation conditions are briefly reviewed.
      
  optimization of fermentation conditions
Optimization of fermentation conditions for P450 BM-3 monooxygenase production by hybrid design methodology
      
Optimization of fermentation conditions for production of glycopeptide antibiotic vancomycin by Amycolatopsis orientalis
      
Overproduction of a recombinant carboxypeptidase inhibitor by optimization of fermentation conditions
      
Here we show that optimization of fermentation conditions by applying statistically designed, multifactorial experiments offers an additional method for potential enhancement of gene expression systems.
      
Optimization of fermentation conditions for production of exopolysaccharide by Bacillus polymyxa
      
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Sisomicin high producer NS8-30 was isolated by conventional mutation breeding. As a result.the production of strain NS8-30 was increased by more than 17-fold,and was investigated in 5-ton fermentor.The blocked mutant 7-25 was obtained. By separating and purifying with IRC-50, antibiotic 7-25 was obtained as major component.It indicates that antibiotic 7-25 is identical with JI-20A by spectroscopy analysis.

用常规诱变育种方法对西索米星小单孢菌进行诱变育种,获得NS8-30菌株,结合发酵条件研究,发酵效价比原始菌株提高17倍以上,并在中试生产中应用。获得1株阻断型突变株7-25,其代谢产物经光谱学分析鉴定为JI-20A,并对其代谢途径进行了讨论。

Dihydropyrimidinase in intact cells of Pseudomonas putida D has high specificity for 5(4hydroxyphenyl)hydantoinOptimum conditions for growth and dihydropyrimidinase formation of Pseudomonas putida D were defined:yeast extract is an essential factor for enzyme formation5Methylthioethylhydantoin was found to be a strong effective inducer as wellSuitable medium consists of yeast extract 10%,glycerol 10%,K2HPO43H2O 02%,NaCl,03%,5methlythioethylhydantoin 01% and MgCl26H2O 005%When the organism was growth at 28 for...

Dihydropyrimidinase in intact cells of Pseudomonas putida D has high specificity for 5(4hydroxyphenyl)hydantoinOptimum conditions for growth and dihydropyrimidinase formation of Pseudomonas putida D were defined:yeast extract is an essential factor for enzyme formation5Methylthioethylhydantoin was found to be a strong effective inducer as wellSuitable medium consists of yeast extract 10%,glycerol 10%,K2HPO43H2O 02%,NaCl,03%,5methlythioethylhydantoin 01% and MgCl26H2O 005%When the organism was growth at 28 for 18h,about 226units/ml of dihydropyrimidinase was otained

通过筛选确定由恶臭假单胞(Pseudomonasputida)D菌产生的二氢嘧啶酶对5-对羟基苯海因的水解能力较强,该产酶菌发酵条件的研究表明酵母膏对产酶十分重要。甲硫乙基海因对二氢嘧啶酶具有强诱导作用。此产酶菌最适培养基组成为(%):甘油1.0,酵母膏1.0,NaCl0.3,K2HPO4·3H2O0.2,甲硫乙基海因0.1,MgCl2·6H2O0.05,28℃,发酵18h,每ml发酵液酶活力为2.26U。

Based on PCR techniques, a gene encoding penicillin G acylase(PGA) was obtained from genomic DNA of Providencia rettgeri(A.TCC29944). The pET24a vector combined with the PGA gene was transformed into BL21(DE3) and the resulting recombinant Escherichia coli gave a high level expression of PGA. Several factors, including IPTG concentration, temperature and carbon source, were optimized for enzyme overproduction. The optimization significantly increased PGA expression by 66-fold compared to the native expression...

Based on PCR techniques, a gene encoding penicillin G acylase(PGA) was obtained from genomic DNA of Providencia rettgeri(A.TCC29944). The pET24a vector combined with the PGA gene was transformed into BL21(DE3) and the resulting recombinant Escherichia coli gave a high level expression of PGA. Several factors, including IPTG concentration, temperature and carbon source, were optimized for enzyme overproduction. The optimization significantly increased PGA expression by 66-fold compared to the native expression (15U/L) in P. rettgeri. After inducing with 1. 0mmol/L IPTG at 24! and fermentation in medium with additive of 5g/L glycerol for 1.5 hour, The enzyme expression of recombinant E. coli reached 993.4U/L.

利用PCR和分子克隆技术从雷氏普罗威登斯菌(Providencia rettgeri)(ATCC29944)的基因组DNA中获得一个青霉素G酰化酶(penicillin G acylase,PGA)基因并将其装入表达质粒pET24a。携带有重组质粒pETPGA的Escherichia coli基因工程菌BL21(DE3)/pETPGA实现了PGA的高效表达。对发酵条件的研究表明基因工程菌在24℃、添加5g/L甘油条件下以1.0mmol/L IPTG诱导1.5h酶活力即达到993.4U/L,比野生菌酶活力(15U/L)提高了66倍。

 
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