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   非磷酸化 的翻译结果: 查询用时:0.023秒
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非磷酸化
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  non-phosphorylation
     The level of tau phosphorylation was detected by Western blot and immunohistochemistry using sites specific antibodies (PHF-1 and Tau-1), and it was normalized by non-phosphorylation dependent total tau antibody (111e).
     应用磷酸化位点特异性抗体(PHF-1和Tau-1)作免疫印迹和免疫组织化学检测tau蛋白的磷酸化水平,并用非磷酸化依赖的总tau蛋白抗体(111e)进行标准化。
短句来源
     At ser-199/202 site and ser-404 site, the phosphorylation of tau protein in OA-treated cells was significantly increased compared with the normal group, and the non-phosphorylation of tau protein was obviously decreased.
     OA模型组tau蛋白ser199/202和ser404位点磷酸化水平较正常对照组明显增高,非磷酸化水平下降;
短句来源
     Preincubation of COIG (100 and 200 mg·L -1) with SK-N-SH cells prevented above changes induced by OA, including that the cell morphology maintained normal in COIG groups, phosphorylation level was significantly decreased and non-phosphorylation level was increased at ser-199/202 site and ser-404 site of tau protein, and the area of microtubules was obviously enlarged.
     COIG(100和200mg·L-1)给药组细胞形态基本恢复正常,tau蛋白ser199/202和ser404位点磷酸化水平较OA模型组明显下降、非磷酸化水平升高,细胞微管平均面积比模型组明显增大。
短句来源
     The diverse doses of OA were incubated with SK-N-SH cells for different periods of time. The changes incell morphology were observed by microscope. The phosphorylation and non-phosphorylation level of tau protein atSer202 site and Ser404 site were detected with Western blotting method.
     用不同剂量OA与SK-N-SH细胞共温育不同时间,用显微镜观察细胞形态变化,用Western印迹法检测磷酸化tau蛋白和非磷酸化tau蛋白在Ser202位点和Ser404位点磷酸化水平的变化。
短句来源
  “非磷酸化”译为未确定词的双语例句
     The correlation between the two pathological alterations was positive at PHF-1 site(r=0.97, P<0.05), negative at Tau-1 site(r=-0.963, P<0.05), and not significant at pS214 site(r=0.705, P>0.05).
     大鼠空间记忆保留障碍与海马tau蛋白PHF-1位点的磷酸化程度呈正相关(r=0·97,P<0·05),与Tau-1位点的非磷酸化程度呈负相关(r=-0·963,P<0·05),与pS214位点的磷酸化程度无相关性(r=0·705,P>0·05)。
短句来源
     ③ Cardiac nuclear binding ac tivity of nuclear inositol 1,4,5 trisphlospate receptor:In the sham operated control group,there was no obvious difference between the phosphorylation and n on phosphorylation subgroups[(102.83± 0.09),(106.24± 0.26) pmol/g,P >0.05].
     ③大鼠心肌细胞核膜三磷酸肌醇受体的结合力:磷酸化组和非磷酸化组无明显差异犤(102.83±0.09),(106.24±0.26)pmol/g,P>0.05犦。
短句来源
     ② Cardiac nuclear binding activity of nuclear inositol 1,4,5 trisphlospate receptor:In t he ischemia reperfusion group,it was obviously lower in the phosphorylation subgroup than in the non phosphorylation subgr oup[(88.27± 0.08),(136.05± 0.05) pmol/g,P< 0.05].
     ②大鼠心肌细胞核膜三磷酸肌醇受体的结合活性:缺血再灌注组与磷酸化组明显低于非磷酸化组犤(88.27±0.08),(136.05±0.05)pmol/g,P<0.05犦。
短句来源
     Expression of IκBα protein(including IκBα-p and IκBα-n) in liver and pancreas were also higher in AP group as compared with that in control group(P<0.05).
     与IκBα的mRNA表达相一致,肝脏和胰腺组织中IκBα蛋白表达(无论其磷酸化形式和非磷酸化形式)在AP大鼠均高于正常对照组(P<0.05);
短句来源
     When 10 nmol/L OA was incubated with SK-N-SH cells for6-24 h,the expression of phosphorylated tau protein at Ser199/Ser202 site and Ser404 site was increased,theexpression of non-phosphorylated tau at Ser202 site and Ser404 site was decreased,and the total tau protein did notshow obvious changes.
     10nmol/LOA与SK-N-SH细胞温育6~24h,磷酸化tau蛋白Ser199/Ser202位点和Ser404位点的表达明显增高,非磷酸化tau蛋白Ser202位点和Ser404位点的表达明显降低,总tau蛋白含量无明显变化。
短句来源
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  相似匹配句对
     The total ERK and CREB had no significant changes.
     磷酸化的CREB和ERK没有显著变化。
短句来源
     Signal Pathway of Migration of Smooth Muscle Cells in Myosin Light Chain Without Phosphorylaion
     平滑肌细胞迁移的肌球蛋白轻链磷酸化途径
短句来源
     PHOSPHORYLATION OF SOY PROTEIN
     大豆蛋白磷酸化
短句来源
     Bronze in Lijiashan
     主流的青铜
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     After sars
     典后遗症?
短句来源
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  non-phosphorylation
MAb AMC3O recognizes a non-phosphorylation-dependent epitope of an as yet unidentified antigen.
      
Thus, in the absence of phosphorylation by PKA, CREB may activate transcription via other non-phosphorylation-dependent mechanisms.
      


Ca2+/CaM PK II has first been purified from bovine brain to homog-enety on SDS-PAGE by using combination of several column chromatographies. The molecular weight of the holoenzyme of the kinase determined by gel filtration was 550 kD and the subunit molecular weight determined by SDS-PAGE was 55 kD. The kinase therefore consisted of ten identical subunits. The kinase activity showed an absolute dependence on Ca2+ and CaM:the AC50 were 0.85 μmol/L and 0.18 μmol-/L using 63 kD PDE isozyme as a substrate respectively;...

Ca2+/CaM PK II has first been purified from bovine brain to homog-enety on SDS-PAGE by using combination of several column chromatographies. The molecular weight of the holoenzyme of the kinase determined by gel filtration was 550 kD and the subunit molecular weight determined by SDS-PAGE was 55 kD. The kinase therefore consisted of ten identical subunits. The kinase activity showed an absolute dependence on Ca2+ and CaM:the AC50 were 0.85 μmol/L and 0.18 μmol-/L using 63 kD PDE isozyme as a substrate respectively; the AC50 were 0.22UUUUmol/L and 0.06μmol/L L.Using casein as a substrate respectively Bovine brain Ca2+/CaM PK Ⅱ was found to autophosphorlate in addition to phosphorylating 63 kD PDE isozyme and the other protein or enzymes.The phosphorylation of 63 kD pDE isozyme by the bovine brain Ca2+/CaM PK Ⅱ resulted in the maximal incorporation of 1 mol phosphate per mol subunit of 63 kD PDE isozyme. The AC50 for Ca2+ of the phosphory-lated form of the 63 kD PDE isozyme was higher than that of the nonphosphoryla-ted form of the 63 kD PDE isozyme.

通过一系列层析法,首次从牛脑纯化得到胶凝电泳匀一的Ca~(2+)/CaM PKⅡ。凝胶过滤法测定全酶分子量为550kD,SDS-PAGE法测定亚基分子量为55kD,推测牛脑Ca~(2+)/CaM PK Ⅱ由十个相同的亚基组成。该酶活性绝对依赖于Ca~(2+)和CaM,以63kD PDE同工酶为底物,其AC_(50)分别为0.85μmol/L和0.18μmol/L;以酪蛋白为底物,其AC_(50)分别为0.22μmol/L和0.06μmol/L。牛脑Ca~(2+)/CaM PK Ⅱ旣能催化63kD PDE同工酶等多种蛋白或酶磷酸化,又能进行自身磷酸化。该酶催化63kD PDE同工酶最大磷酸参入量为1mol/mol亚基。磷酸化型63kD PDE同工酶的Ca~(2+)的AC_(50)高于非磷酸化型。

In this paper the regulation of the activity of bovine brain 63kD PDE isozyme by phosphorylation and dephosphorylation was studied. The experimental results are as follows: 1. The 63kD PDE isozyme was phosphorylated by the purified bovine brain Ca~(2+)/CaM-PK Ⅱ in the presence of Ca~(2+) and CaM, the maximal phosphate incorporation was 1mol/mol subunit of the 63kD PDE isozyme. 2. The phosphorylated 63kD PDE isozyme was dephosphorylated by calcineurin in the presence of Ni~(2+) and CaM. 3. The AC_(50) for Ca~(2+)...

In this paper the regulation of the activity of bovine brain 63kD PDE isozyme by phosphorylation and dephosphorylation was studied. The experimental results are as follows: 1. The 63kD PDE isozyme was phosphorylated by the purified bovine brain Ca~(2+)/CaM-PK Ⅱ in the presence of Ca~(2+) and CaM, the maximal phosphate incorporation was 1mol/mol subunit of the 63kD PDE isozyme. 2. The phosphorylated 63kD PDE isozyme was dephosphorylated by calcineurin in the presence of Ni~(2+) and CaM. 3. The AC_(50) for Ca~(2+) of the phosphorylated form of the 63kD PDE isozyme was higher than that of the nonphosphorylated form of the 63kD PDE isozyme.

本文报道了CaM依赖性磷酸化和脱磷酸化对牛脑63kD PDE同工酶活性的调节作用。实验结果如下:(1)在存在Ca~(2+)和CaM时,提纯的牛脑Ca~(2+)/CaM-PK Ⅱ能催化牛脑63kD PDE同工酶磷酸化。最大磷酸参入量是1mol/mo1亚基;(2)在Ni~(2+)和CaM存在时,提纯的牛脑钙调神经磷酸酶能催化磷酸化型63kDPDE同工酶脱磷酸化;(3)CaH~(2+)对磷酸化型63kD PDE同工酶的半激活浓度(AC_(50))高于非磷酸化型。

Our previous study has shown that there are four kinds of act in binding protein(ABP) in the human blood platelet, ABP0, ABP1, ABP2and ABP3. which are phosphorylated to different extents. The scanning results of SDS-PAGE of ABP-aotin complexes show that the binding ability of ABP3 to aotin is strongest, ABPO has no aotin-binding ability. ABP1 and ABP2 bind to actin to similar extent.Using an eleotroeluting technique, we have successfully purified ABP and its proteolytic products, Mr 190 ×103 and 90 ×103 fragments,...

Our previous study has shown that there are four kinds of act in binding protein(ABP) in the human blood platelet, ABP0, ABP1, ABP2and ABP3. which are phosphorylated to different extents. The scanning results of SDS-PAGE of ABP-aotin complexes show that the binding ability of ABP3 to aotin is strongest, ABPO has no aotin-binding ability. ABP1 and ABP2 bind to actin to similar extent.Using an eleotroeluting technique, we have successfully purified ABP and its proteolytic products, Mr 190 ×103 and 90 ×103 fragments, by calcium-dependent sulfhydryl protease (CDSP). Results of amino acid sequencing show that the N-terminal of ABP is blocked, but the 190 ×103 fragment has an N-terminal sequece of ATEKDLAEDAP, which is the same as the T110 fragment in human smooth muscle filamin.These results together with our previous results suggest that: (1) It is neccessary for binding to actin that ABP is phosphorylated by PKO and PKA. (2) The binding ability of ABP to aotin increases with the extent of its phosph-orylation. (3) Aotin-binding proteins in the human blood platelet and smooth muscle could be homologous.

我们以前的研究显示人血小板中存在4种磷酸化程度不同的肌动蛋白结合蛋白:ABP0、ABP1、ABP2和ABP3。电泳扫描结果表明:完全磷酸化的ABP3结合肌动蛋白能力最强,约为部分磷酸化的ABP(ABP1和ABP2)的2倍。ABP1和ABP2具有相似结合能力,而非磷酸化的ABP0几乎失去结合肌动蛋白的能力。利用电泳洗脱法我们分离到电泳纯的ABP及其他的钙依赖巯基蛋白酶(CDSP)分解产物,Mr190×10~3、90×10~8两个片段。氨基酸顺序分析结果显示ABP分子的N-端被封闭,190×10~3片段N-端的前1个氨基酸顺序为ATEKDLAE OAP,与人平滑肌细胞的细丝蛋白分子中T110片段相同。 以上结果提示:(1)cAMP调控蛋白激酶(PKA)和蛋白激酶G(PKC)的磷酸化对ABP结合肌动蛋白是必须的;(2)ABP结合肌动蛋白的能力随磷酸化程度增强而增加;(3)血小板和平滑肌细胞的肌动蛋白结合蛋白可能具有同源性。

 
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