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高酶活
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  high enzyme activity
     Study on The Fermentative Conditions of Liquid Deep Fermentation of High Enzyme Activity Pectinase YPC-16
     高酶活果胶酶突变株YPC—16液态深层发酵条件的研究
短句来源
     Three fungi strains with high enzyme activity on selective degradating lignin of traw were got by methods of PDA-Bavendamm,PDA-RB and color-zone,which are Pleurotus ostreatus,Coriolus versicolor and Trametes gallica.
     通过PDA-Bavendamm平板显色反应、PDA-RB亮蓝显色反应、变色圈试验,获得选择性降解稻草木质素的高酶活菌株:糙皮侧耳、彩绒革盖菌和粗毛栓菌。
短句来源
     U.V. Inducing and Screening Tests of High Enzyme Activity Strains in Degumming
     大麻脱胶高酶活菌株的诱变选育
短句来源
     So it is important that to produce NHase which had high enzyme activity and maintain its stability.
     催化所需要的酶是影响产率的一个重要因素,高酶活的睛水合酶的生产及其稳定性的保持是极其重要的。
短句来源
     Then cloning them into expression vector pBL and successfully expressing them with high enzyme activity in \%E. coli\% DH5α.
     用于表达的片段定向连接到表达载体 pBL并转化大肠杆菌DH5α后 ,获得高酶活表达。
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  “高酶活”译为未确定词的双语例句
     Treating the soybeans at 70℃,pH(5.0) for 24 h,20 cultivars from the 45 cultivars with high enzymatic activity keep 50% SBP activity,indicating a good thermostability.
     SBP热稳定性优异,经70℃(pH5.0)处理24 h,45份高酶活品种中有20份剩余酶活超过50%的。
短句来源
     343 strains isolated from soil and active mud samples effectively primarily screened with this method and after re-screened with shaker fermentation to test their enzymatic activity,6 strains of hi-ODC activity were obtained,among them the activity of CJW07 and CGW27 reached up to 121.32 U/mL and 109.25 U/mL respectively.
     对从土壤和活性污泥样品中分离得到的343株细菌进行有效初筛以及经过摇瓶发酵测定酶活的复筛试验后,筛选得到6株高酶活的菌株,其中菌株CJW07和CGW27的ODC活性分别达到了121.32 U/mL和109.25 U/mL。
短句来源
     IDENTIFICATION OF CELLULOSE-DECOMPOSING FUNGUS-SU97 AND HIGH ACTIVITY STRAINS-SELECTION
     纤维素分解菌SU_(97)的鉴定及高酶活菌株选育
短句来源
     after registered on Genebank five gene numbers were given:AY645667,AY645668,AY645559,AY645670,AY645671.The five new sequences were aligned with the the original gene by the biological software DNAman.
     经过多轮诱变,得到五个具有高酶活的诱变基因,并在Genebank上注册,序列号为AY645667,AY645668,AY645669,AY645670,AY645671。
短句来源
     Classifying soybean cultivars into high and low activity groups with a critical value of 100U/g,the high activity group accounts for about 1/3 of total cultivars tested.
     根据SBP含量高低,大豆可分为高酶活与低酶活二大类型,分类临界值为100U,高酶活类约占1/3。
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  相似匹配句对
     V. C.
     V.C;
短句来源
     semitecum was capable to produce some higher Cellulase activity than R.
     stolonifer产的
短句来源
     Production Experiment on High Activity Glucoamylase
     力糖化生产试验
短句来源
     The activities of enzymes changed obviously under the high concentration.
     浓度下变化明显。
短句来源
     The Km value indicates that the enzyme has very high affinity for starchby comparison with that of the previously reported.
     这比文献报道的
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  high enzyme activity
cerevisiae produced very high enzyme activity and showed to be the most effective procedure to obtain glucose-6-phosphate dehydrogenase.
      
By contrast, a relatively high enzyme activity was found for TfdFII.
      
Further, p53 mutations among smoking cases were less frequent in the group with a putative high enzyme activity, although insignificant due to small numbers (OR 0.54, 95% CI 0.13-2.17).
      
The calculated cyclic voltammograms showed prepeaks when there was a low substrate concentration, high mediator concentration, and high enzyme activity.
      
A very high enzyme activity was found only in the less well differentiated HI-F tumor line.
      
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The nitrate reductase preparation from rice leaves was purified by affinity chromatography. Two peaks of NADH: nitrate reductase activity were found when the enzymes was eluted from Blue Dextran-Sepharose 4B affinity column by 100μM NADH and 0.25 M KNOa solution successively. These two fractions of nitrate reductase were designated as NADH- and KNO_3-eluted nitrate reductase respectively. This suggested that nitrate reductase was bound to the affinity column through two binding sites: One was a NADH-binding...

The nitrate reductase preparation from rice leaves was purified by affinity chromatography. Two peaks of NADH: nitrate reductase activity were found when the enzymes was eluted from Blue Dextran-Sepharose 4B affinity column by 100μM NADH and 0.25 M KNOa solution successively. These two fractions of nitrate reductase were designated as NADH- and KNO_3-eluted nitrate reductase respectively. This suggested that nitrate reductase was bound to the affinity column through two binding sites: One was a NADH-binding site, and the other was a NO_3-binding site. In eluting nitrate reductase from the affinity column, the nitrate may act either by increasing the ionic strength or through a substrate effect, or both. When KNO_3-eluted nitrate reductase was collected and again passed through the affinity column for the second or the third time, there were still two peaks of nitrate reductase, but the ratio of the activity of KNO_3-eluted nitrate reductase to that of NADH-eluted one decreased. After the third time of affinity chromatography, very little KNO_3-eluted nitrate reductase activity remained. However, by means of 7.5% polyacrylamide gel and SDS-polyacrylamide gel electrophoresis, both NADH- and KNO_3-eluted nitrate reductase showed the same electrophoresis pattern.

水稻叶片NR在Blue Dextran-Sepharose 4 B亲和层析法的纯化过程中,继100μMNADH洗脱出现一个酶活峰后,再用0.25MKNO_3洗脱又出现一个更高的酶活峰。酶与BlueDextran-Sepharose 4B有两个结合位点,它们可能就是酶与底物NADH和NO_3的结合位点。NADH能和Blue Dextran竞争与酶结合而把NR从亲和柱上洗脱下来。硝酸盐除了具一般盐类的离子强度外,还有明显的底物效应。如果将KNO_3-洗脱的NR再反复进行亲和层析,又可得到NADH-洗脱和KNO_3-洗脱的两部分NR。这两部分NR在聚丙烯酰胺凝胶电泳和SDS-聚丙烯酰胺凝胶电泳上都呈现一条相同的蛋白带。 水稻NR的全酶分子量约330Kd,亚基分子量约57Kd,故全酶可能由6个相同亚基组成,酶活的pH范围为6.5~8.5,最适pH为7.5;酶对底物NO_3-和NADH的K_m值分别为3.3×10~(-4)M和2.9×10~(-5)M。

By complex mutagenesis of Asp. usamii B_1 with N(?)G, UV—raysand LiCl, a black-spore mutant strain, B_(1-12), was selected. The strain is Suitable for liquid culturatation with a shorter fermentation circle and optimum pH shift to a lower pH value. The activity of exo-PG increases while the activity of glucoamylase decreases. But the stability of pectic enzymes at 60℃ decreases. The mutant strain is superior to B_1 in clarifying several kinds of fruit juices.

B_(1—12)是由Asp·usamii B_1经NTG、UV、LiCl复合处理选育到的一株呈黑色的孢子是适于液体培养的高酶活果胶酶菌株。具有发酵周期短、易过滤等痔点,酒樁沉淀的酶制剂与B_1比较,酶作用的最适pH分别为4.0和4.5,最适温度均力50℃,但在30℃时,B_1仍保持较高酶活水平的稳定性。B_(1—12)果胶酶组分ex—PG酶活高于B_1 0.5—1倍左右,endo—PG和PE变化不明显.两者都含有糖化酶、纤维素酶、蛋白酶、花青素酶等非果胶酶组分,其中糖化酶活性比B_1下降一倍.应用于苹果醋、苹果汁、草莓汁、樱桃汁的澄清.B_(1—12)优于B_1,透光率可达90%以上,但对枣汁的澄清效果却次于B_1。

Plant oligosaccharides were enzymatically extracted from wheat cell walls. After their bioactivity analyses, the oligosaccharides were proved to possess functions upon induction of both soybean and wheat phytoalexins and improvement of wheat defence at its metabolism, cell and plant levels. Bioactivity of wheat oligosaccharide was closely correlated with variety, purification arid active unit of the hydrolytic enzymes used? strongly biofunctional oligosaccharides could be released by enzymes of high purification...

Plant oligosaccharides were enzymatically extracted from wheat cell walls. After their bioactivity analyses, the oligosaccharides were proved to possess functions upon induction of both soybean and wheat phytoalexins and improvement of wheat defence at its metabolism, cell and plant levels. Bioactivity of wheat oligosaccharide was closely correlated with variety, purification arid active unit of the hydrolytic enzymes used? strongly biofunctional oligosaccharides could be released by enzymes of high purification and active unit. Three kinds of oligosaccharides from different sources were compared in their biofunctions, while other factors were also discussed about their influence on oligosaccharide bioactivity, such as the physiological status of wheat cells and the conditions to preserve oligosaccharides.

应用酶解法从小麦细胞壁中提取出低聚糖。经过活性鉴定,具有诱导大豆和小麦抗毒素产生,特别是从小麦代谢、细胞、植株等水平上改善该作物体内防御功能等生物作用。水解酶的种类、纯度、酶活值与所降解低聚糖的活性程度密切相关;高纯度、高酶活单位的水解酶提取的低聚糖,具有较强的生物功能。对来源不同的低聚糖进行了活性差异的比较,同时还讨论了其它因素——诸如小麦细胞的生理状态,低聚糖保存条件等对低聚糖活性的影响。

 
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