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   固定化酶活 的翻译结果: 查询用时:0.262秒
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固定化酶活
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  yield of immobilized enzyme
     The maximum yield of immobilized enzyme was 30.2 %.
     固定化酶活最高回收率为30.2%.
短句来源
  “固定化酶活”译为未确定词的双语例句
     The maximum activity 10.6 IU/mg is obtained with 88.47% activity recovery at 1.0% polymer concentration.
     在1.0%EudragitL-100浓度时获得10.6IU/mg载体的固定化酶活和88.47%的酶活回收。
短句来源
     The results showed that the maximum 0.44U/cm 2activity of the immobilized lipase was given when the benzoquinone content was 0.01g/ml,activation time was 60min,lipase content in pH 8.0 phosphate buffer was 0.005g/ml and crosslinking reaction time was 24h at 4℃.
     结果表明,当对苯醌浓度为0.01g/ml,活化60min,与浓度为0.005g/ml的酶的pH8.0的磷酸盐缓冲溶液于4℃交联24h,获得的固定化酶活最高,为0.44U/cm2。
短句来源
     The immobilized activity of prepared support for PGA is 1.47 folds to that of Eupergit C, and 2.2 folds to those of Sepabeads Ec-Ep and Amberzyme oxirane resin.
     固定化青霉素G 酰化酶固定化酶活是Eupergit C 的1.47 倍,是Sepabeads Ec-Ep 和Amberzyme 环氧树脂的2.2 倍。
短句来源
     Our results showed that the maximum 0.26U/cm\+2 activity of immobilized lipase was given when the content of glutaraldehyde was 5%,the activitied time was 12h,the temperature and pH of crosslinking reaction was at 15 and 7.6 and the crosslinking reaction time was 12h.
     结果表明,当戊二醛浓度为5%,活化12h,与pH7.6的酶的磷酸盐缓冲溶液于室温(15℃)交联12h,获得的固定化酶活最高,为0.26U/cm2。
短句来源
     The activity recovery of immobilized GST reached 41.6%, optimal pH 6.5~7.0, optimal temperature 37℃ and the thermal stability of immobilized GST wasenhanced.
     结果固定化酶活回收率可达41.6%,最适pH6.5~7.0,最适温度37℃,对底物(CDNB和GSH)的亲和力略有下降,对温度稳定性大大提高。
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  相似匹配句对
     Review of current research on immobilized enzyme
     固定化研究进展
短句来源
     Studies on Immobilization of Enzyme and Cells
     和细胞的固定化研究
短句来源
     Effect of ultrasound on activities of immobilized enzymes
     超声波对固定化性的影响
短句来源
     Activity and Stability of Immobilized Enzyme in the Formation of L Phenylalanine
     固定化细胞合成L-苯丙氨酸的及稳定性
短句来源
     Study on production of casein phosphopepfides by Immobilized Enzyme
     固定化生产性肽CPP研究
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  yield of immobilized enzyme
The activity yield of immobilized enzyme was approximately 55% of the free enzyme.
      
The yield of immobilized enzyme protein was determined as 85% and the immobilized biocatalyst retained 97% of the initial chitosan-invertase activity.
      


Anion exchanger DEAE—celluiose was pretreated and equilibrated in phosp.hate buffer,then with the addition of glucose isomerase(GI)solution was stirredfor 0.5 hr.The supernatant was filtrated and the DEAE—Cellulose—GI Complexwas washed three times with phosphate buffer.The activity per gramme of imm-obilized GI is about 5,000—6,000 u.The adsorbent ability of DEAE—cellulose may be improved with the properincrease of en yme concentration or with a primary purification of the enzyme.The activity of immobilized...

Anion exchanger DEAE—celluiose was pretreated and equilibrated in phosp.hate buffer,then with the addition of glucose isomerase(GI)solution was stirredfor 0.5 hr.The supernatant was filtrated and the DEAE—Cellulose—GI Complexwas washed three times with phosphate buffer.The activity per gramme of imm-obilized GI is about 5,000—6,000 u.The adsorbent ability of DEAE—cellulose may be improved with the properincrease of en yme concentration or with a primary purification of the enzyme.The activity of immobilized GI comes up to about 10,000 u/g.

DEAE—纤维素阴离子交换剂经预处理,再用磷酸缓冲液平衡,然后加入葡萄糖异构酶溶液,搅拌吸附半小时.把上清液滤去,得DEAE—纤维素—葡萄糖异构酶复合物,用磷酸缓冲液洗三次.每克固定化酶活性为5,000—6,000单位.如果适当增加酶浓度,或把异构酶初步纯化,DEAE—纤维素吸附葡萄糖异构酶的量可以增加,固定化酶活性能高达10,000单位/克左右.

In this paper,the optimum conditions of crosslinking porcine pancreas lipase to chitosan coat carrier were studied.Our results showed that the maximum 0.26U/cm\+2 activity of immobilized lipase was given when the content of glutaraldehyde was 5%,the activitied time was 12h,the temperature and pH of crosslinking reaction was at 15 and 7.6 and the crosslinking reaction time was 12h. The optimum operating temperature and pH of gained immobilized lipase was at 40 and 8.5 respectively.

研究了壳聚糖涂层在纤维素滤纸上成膜后再固定化猪胰脂肪酶的最佳条件。结果表明,当戊二醛浓度为5%,活化12h,与pH7.6的酶的磷酸盐缓冲溶液于室温(15℃)交联12h,获得的固定化酶活最高,为0.26U/cm2。固定化酶最适温度40℃,比游离酶提高了5℃;最适pH8.5,与游离酶相比,向碱性偏移了0.5个pH

The conditions of crosslinking porcine pancreas lipase to cellulose membrane carrier by means of benzoquinone were studied.The results showed that the maximum 0.44U/cm 2activity of the immobilized lipase was given when the benzoquinone content was 0.01g/ml,activation time was 60min,lipase content in pH 8.0 phosphate buffer was 0.005g/ml and crosslinking reaction time was 24h at 4℃.The optimum operating temperature and pH of the immobilized lipase was 35℃ and 9.0 respectively.

研究了对苯醌活化法在纤维素滤纸膜载体上固定化猪胰脂肪酶的最佳条件。结果表明,当对苯醌浓度为0.01g/ml,活化60min,与浓度为0.005g/ml的酶的pH8.0的磷酸盐缓冲溶液于4℃交联24h,获得的固定化酶活最高,为0.44U/cm2。固定化酶最适温度35℃,最适pH9.0。

 
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