A rapid, sensitiive and specific enzyme-linked immunosorbent assay (ELISA)method, using mouse monoclonal human thyroglobulin antibodies, was established in our laboratory for measuring human serum thyroglobulin with a sensitivity of 1ng/mL.
Different concentrations(0%,2.5%,5%,10%,20%)of HPS (human placental culture supernatants) were added to IgG hybridoma culture (H 4) and antithyroid globulin protein antibody was collected from the supernatants after being cultured for 24h,48h,72h,96h to examine the antibody production by ELISA.
本研究在培养分泌抗甲状腺球蛋白单克隆抗体( Ig G 型) 杂交瘤 H4 株时, 加入不同浓度(0 % 、2 .5 % 、5 % 、10 % 、20 % ) 的人胎盘培养上清液( H P S) ,培养不同的时间(24h 、48h 、72h 、96h)后取上清液,用 E L I S A 法检测各培养上清液中抗体含量。
The antithyroid antibodies (thyroglobulin antibodies, microsomal antibodies) showed normal titres and did not suggest disturbances of thyroid autoimmunity in the patients with ALS.
After immunized by pTG, the mice were sacrificed on day 28 and day 38 to examine their thyroid gland pathologically, and to check the levels of serum porcine thyroglobulin antibodies (pTGAb), porcine thyromicrosomal antibodies (pTMAb).
Antibodies against human IgG, C3 and monoclonal antibody against Human thyroglobulin were used to detect the thyroglobulin immune complex on the erythrocyte membrane of 48 patients with thyroid disease, by a immunofluoresence assay The specific fluorescence was found on the erythrocyte membrane of 14 patients with Graves' disease and Hashimoto's thyroiditis. The immunopathogical signification of this immune complex was also discussed in this paper.
By using enzyme-linked immunosorbent assay(ELISA),the authors studied theeffects of temperature,pH and ionic strength on the activity of monoclonal anti-human thyroglobulin antibody F2,with the comparison of purified McAb,ascites and rabbit antiserum.The results showed that McAb and antiserum all had the high-est activity at 37℃,but at 65℃,the activity of the McAb was higher than anti-serum,while purified McAb was more sensitive to high temperature than ascites.ThepH effect study showed that the activity of...
本文报导纯化和不纯化的抗人甲状腺球蛋白单克隆抗体 F 2受 pH、温度和离子强度等影响的情况,并与兔抗血清(多克隆抗体)进行比较。纯化单抗对这些因素比腹水敏感性大。研究还强调了研究 pH 影响需注意离子强度。
Messenger RNA was prepared from hybridoma secreting monoclonal antihuman thyroglobulin antibodies. Micro-injection of the mRNA into frog oocyteS resulted in increasing of protein synthesis detected by incorporation of 3H-Leu. the immunological activity of antibodies against thyroglobulin was proved in the products of oocytes after mRNA injection by immuno- precipitation.