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冷乙醇
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  cold ethanol
     Methods HL 60 or Molt 4 cells were induced by CAM, AMSA or VIN in vitro for 3,6,9? h,then the cells were fixed with 75% cold ethanol over 24?
     方法 在体外用喜树碱、胺苯吖啶或长春花碱处理诱导HL 60或Molt 4细胞 3、6、9h后收获 ,75 %的冷乙醇固定 2 4h以上。
短句来源
     Purification of Human Serum Albumin from Plasma with the Combination of Hydrophobic Interaction Chromatography and Cold Ethanol Precipitation
     疏水层析结合冷乙醇沉淀纯化人血清白蛋白
短句来源
     The content of saturated long chain fatty acid(C-(20:0),C-(22:0),C-(24:0)) of peanut oil is about 6%~7%. The foundation of the qualitative detection of peanut oil is that those fatty acids can't dissolve in cold ethanol.
     与一般食用油脂相比,花生油含有6%~7%的长碳链饱和脂肪酸(20:0、22:0、24:0),具有不溶于冷乙醇的特殊性质,是定性鉴别花生油的基础.
短句来源
     Cold ethanol (75 %) fixed tumor cells (HL-60 and Molt-4) were lysised and protein extracted under room temperature or hypothermia. Then sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of protein with subsequent Western blotting was performed.
     于低温 (4℃ )或常温条件下裂解经75 %冷乙醇固定的肿瘤细胞 (HL- 6 0细胞和 Molt- 4细胞 ) ,提取细胞蛋白质进行 SDS- PAGE凝胶电泳和 Western印迹。
短句来源
     15 male goats developed type **********I or *******II DCS after compression and decompression were studied. Plasma FG was extracted from blood by cold ethanol method.
     雄性山羊15只,加压-减压发生Ⅰ或Ⅱ型DCS,采静脉血用冷乙醇提取血浆FG。
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  “冷乙醇”译为未确定词的双语例句
     The yields were 13. 38 %. 9. 68 %, 9. 75 % and 9. 55% respectively.
     三氯乙酸提取法、冷乙醇提取法、热乙醇提取法和热蒸馏水提取法,产率分别为13.38%、9.68%、9.75%和9.55%。
短句来源
     Before add 1.5 times ethanol as the volume of TGase, modify pH of TGase to 7.5 to 8.0. The total recovery of activity of the process is about 85 %.
     将超滤后的酶液pH值调节到7.5至8,加入体积为酶液体积1.5倍的冷乙醇沉淀,酶活收率达到85%左右。
短句来源
     The adhesins of S.mutans serotype c endemic strain WD9463A were extracted from culture superant by cold ethol precipitation, or were extracted from cell surface by 0.5 mol/L phosphate buffer, 6 mol/L urea or 2% SDS solution.
     用冷乙醇沉淀法从变形链球菌培养上清液或用0.5mol/L磷酸盐缓冲液、6mol/L脲和2%SDS溶液从变形链球菌细胞表面分别粗提变形链球菌表面粘结素。
短句来源
     The results showed: the best condition of the enzyme separation with organic solvent is that the ultrafiltrate was concentrated 2.5 to 3.2 times, then adjust the pH to 8, and add 1.5V refrigerant ethanol.
     试验结果表明:超滤的最适浓缩倍数为2 5~3 2; 浓缩液pH值为8,冷乙醇按照酶与乙醇体积比1∶1 5加入为最佳分离条件.
短句来源
     The refined propolis can be extracted by the alcohol from the raw propolis and then dewaxed with cooled alcohol.
     通过试验将蜂胶原料用乙醇提取并用冷乙醇脱蜡得到精制蜂胶。
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  相似匹配句对
     On cold fusion
     核聚变
短句来源
     Cold Mountain
     《山》
短句来源
     2.22 mmol·L~(-1) ethanol was produced.
     L-1的乙醇
短句来源
     Purification of Human Serum Albumin from Plasma with the Combination of Hydrophobic Interaction Chromatography and Cold Ethanol Precipitation
     疏水层析结合乙醇沉淀纯化人血清白蛋白
短句来源
     Mononuclear cells were fixed with 70% cold alcohol, and then incubated with anti ?
     分离的单个核细胞,70%乙醇固定,-20℃保存。
短句来源
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  cold ethanol
Processes for the large-scale fractionation of human plasma using cold ethanol were initially developed by Edwin Cohn and his colleagues at Harvard to provide albumin as a treatment for shock in World War II.
      
This emulsifier was produced optimally in a low-nutrient seawater medium supplemented with glucose and was extractable by cold ethanol precipitation of the high-molecular-weight fraction (>amp;gt;100?kDa).
      
An alternative approach used to study the protein-free fraction is to precipitate the proteins with ice-cold ethanol and then analyze the aliquot.
      
Fixation in cold ethanol and decalcification in EDTa at 4°C preserved the immunological reactivity of the inner ear tissue in use and allowed excellent delineation of its structural details.
      
Preservation of the slides for documentation was improved by fixing the intermediate layer of the antigen and the labeled antibdoy in cold ethanol.
      
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A convenient laboratory method for the preparation of pure fumaropimaric acid from oleoresin of Indian blue pine (Pinus wallichiana) is described.

介绍了山乔松(印度兰松)(Pinus wallichiana)松脂直接制备富马海松酸(FPA)的二种实验室方法:(1)松脂200克、富马酸20克、乙醇1000毫升置三颈瓶中混合并回流48小时,静置过夜、减压吸滤分离出粗FPA,冷乙醇洗数次得纯FPA晶体,190~230℃真空干燥3小时,得率48%,酸价420,熔点295~298℃,[α]D(CHCl_3)±42.5~0;(2)松脂200克、富马酸35克置三颈瓶中在220—225℃下激烈搅拌3小时,由冷却器收集松节油,瓶中粗FPA冷却后加入苯、乙醇混合液(15:85)500毫升,混匀过夜,减压吸滤分离出粗FPA,以冰冷苯、乙醇(15:85)混合液洗数次,得纯FPA纯白色晶体,190—230℃真空干燥2.5小时,得率42%,酸价420,熔点285—287℃,[α]D(CHCl_3)+42.7℃。 纯FPA作了红外、核磁共振、质谱和气液色谱仪器分析予以证实。

In the present paper, the conditions of a porcine leukocyte interferon (PLeIFN) production in porcine leukocytes induced with New Castle Disease Virus strain Ⅱ (NDV Ⅱ ) were described, and the four methods for their purification were compared. The experimental results are as follows: Titer of the interferon obtained was over 1500 units/ml when 40—80 HA units of NDV Ⅱ were added to 1 ml of procine leukocyte suspension. After the porcine leukocytes were pretreated with a low concentration of PLelFN for 3 hours,...

In the present paper, the conditions of a porcine leukocyte interferon (PLeIFN) production in porcine leukocytes induced with New Castle Disease Virus strain Ⅱ (NDV Ⅱ ) were described, and the four methods for their purification were compared. The experimental results are as follows: Titer of the interferon obtained was over 1500 units/ml when 40—80 HA units of NDV Ⅱ were added to 1 ml of procine leukocyte suspension. After the porcine leukocytes were pretreated with a low concentration of PLelFN for 3 hours, the interferon-producing ability of the leukocytes was increased as compared with control; KCNS-cold alcohol method, TCA-eold alcohol method, ammino sulfate-precipitated method and PEG method, were used to purify crude PLeIFN respectively. The results obtained showed that KCNS method was better than the others. Using KCNS method to purify crude PLeIFN without pretreated with acid, a higher efficiency could be obtained.

本文以猪白细胞—鸭新城疫病毒Ⅱ系(NDVⅡ)为诱生系统探讨了猪白细胞干扰素(PLeIFN)诱生条件,并比较了四种方法纯化PLeIFN的效果。实验表明:(1)每毫升猪白细胞悬液加入40~80HA的NDVⅡ,产生PLeIFN效价>1500 u/ml;(2)猪白细胞经低浓度PLeIFN预处理可增强其产生PLeIFN能力,(3)PLeIFN预处理时间以3小时为宜;(4)猪白细胞干扰素在猪肾细胞上的抗病毒活性高于在鸡胚细胞上的活性;(5)四种方法中以硫氰酸钾—冷乙醇法纯化效果为好,该法去除杂蛋白能力高于其它方法;(6)用未酸化处理的PLeIFN粗制品可提高硫氰酸钾—冷乙醇法的纯化效果。

A method for extraction of the large plasmid DNA was developed by improving of Kado-Liu method. The characteristics of this improved method are: Both Tris concentration and pH value of the solution for lysing are higher than that of the original method, 500 mM and 12.8~13.0 respectively; proper amount sodium acetate was used for extraction of proteins in addition to phenol-chloroform; isopropanol was used to concentrate the target DNA at room temperature instead of the cold ethanol. The improved methed showed...

A method for extraction of the large plasmid DNA was developed by improving of Kado-Liu method. The characteristics of this improved method are: Both Tris concentration and pH value of the solution for lysing are higher than that of the original method, 500 mM and 12.8~13.0 respectively; proper amount sodium acetate was used for extraction of proteins in addition to phenol-chloroform; isopropanol was used to concentrate the target DNA at room temperature instead of the cold ethanol. The improved methed showed that it was simple and rapid, and gave rise reproducible results in detection and extraction of plasmid DNA in enterobacterial species.The pH of solution used for lysing can be adjusted directly by adding 930~950μ1 of 10N NaOH (freshly prepared) in 100 ml Tris-SDS solution, which may enable this method more conveniently used in common laboratories.

本文报道了一种源于Kado-Liu法经系列改进后产生的大质粒DNA检测与提取方法。改进之处主要是:裂解液组成中的Tris度为500mM,pH值为12.8~13.0;蛋白质的抽提除了酚—氯仿外同时使用了适量的醋酸钠(3M pH4.8)溶液;以异丙醇室温静置30分钟左右,取代冷乙醇较长时间的低温处理,进行DNA浓缩。该方法在肠道菌方面的应用中,显示了其简易、快速和较好的重复性。另外,用930~950μl新鲜10N NaOH液使裂解液pH值为最佳的经验值,既可避免因不同类型pH计产生不同结果的麻烦,亦为该方法能在普通实验室推广应用提供了方便。

 
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