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临床免疫诊断
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  clinical immunologic diagnosis
     The antibody antihalf-antigen and antigenic can be mass-produced by gene recombination and used in RII for clinical immunologic diagnosis and scientific researches.
     利用基因重组技术大量生产的任何一种相应半抗原和抗原的抗体片段,与RII技术联合,已应用于临床免疫诊断和科学研究。
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  “临床免疫诊断”译为未确定词的双语例句
     CLINICAL IMMUNODIAGNOSTIC SIGNIFICANT OF IgM AND IgG CLASS ANTIBODIES
     戊型肝炎IgM和IgG抗体的临床免疫诊断意义
短句来源
     Studies of clinical immundiagosis of cerebral paragonimiasis
     脑肺吸虫病临床免疫诊断的研究
短句来源
     Studies of Clinical Immundiagosis of Different type Paragonimiasis
     斯氏狸殖吸虫病临床免疫诊断的研究
短句来源
     Objective:To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.
     目的 :建立针对猪囊尾蚴KD2 6抗原的单克隆杂交瘤细胞株 ,生产相应的单克隆抗体 ,用于囊虫病免疫保护机制的基础研究和囊虫病临床免疫诊断的应用。
短句来源
  相似匹配句对
     Studies of clinical immundiagosis of cerebral paragonimiasis
     脑肺吸虫病临床免疫诊断的研究
短句来源
     Establishment of chemiluminescene diagnosis analysis and clinic application in C-peptide
     C肽化学发光免疫诊断分析方法的建立及临床应用
短句来源
     The clinical follow up was performed.
     临床随访。
短句来源
     The evaluation of clinical drug utilization
     临床药物利用评价
短句来源
     Immunodiagnosis of Hydatid Disease of Brain
     脑包虫病的免疫诊断
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To investigate the similarities and differences on the HCV antibodies screening, 2 NCU/ml anti HCV critical value serum and HCV sera panel were detected by five commercial EIA kits, and the C.O.I.(cut off index, i.e. sample OD/cut off OD ratio.), sensitivity and specificity, crude and adjusted agreement, and Youden's index, predictive values of a positive and a negative test, and the distance and ratio of the positive and negative sera OD X— /+ 2SD from the cut off value among the HCV sera panel...

To investigate the similarities and differences on the HCV antibodies screening, 2 NCU/ml anti HCV critical value serum and HCV sera panel were detected by five commercial EIA kits, and the C.O.I.(cut off index, i.e. sample OD/cut off OD ratio.), sensitivity and specificity, crude and adjusted agreement, and Youden's index, predictive values of a positive and a negative test, and the distance and ratio of the positive and negative sera OD X— /+ 2SD from the cut off value among the HCV sera panel were calculated. These results suggested that the sensitivity and specificity had its strong and weak points among the EIA Kits detected, and recommend that the screening in parallel with the different manufacturer's Kits was used for serosurveillance and/or sero immunological diagnosis in order to reduce the proportion of false negative or positive.

本文报道采用和参照检测2NCU/ml抗HCV定值血清C.O.I.是否>1.0、以及检测HCV血清盘按预期结果计算和评价真实性和预示值及调整一致性和约登指数等各项指标,比较与血清盘预期结果列为假阴性和假阳性结果的不一致性和错位现象,计算与比较检出血清盘阳性和阴性OD值的X—±2SD与C.O.值的差值和比值,表明商售抗HCVEIA药盒检测灵敏度和特异度有不同的两种类型,提示供血筛检和临床免疫诊断,宜各取高灵敏度和高特异度两种厂牌试剂,采用配对并联筛检试验方案作双次检测,以减少漏检和误诊。

Objective:To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.Methods:BALB/C mice were immunized 3 times with KD26 antigen of cysticercus cellulosae by SDS PAGE(mixed with Freund's incomplet adjuvant),and the spleen cells of immunized mice were fused with myeloma cell line SP2/0(in the ratio of 10∶1) in the HAT medium containing 50% PEG(4 000 MW),then incubated,selected and cloned.Numerical analysis on chromosome...

Objective:To develop and identify the monoclonal antibody(McAb) against cysticercus cellulosae KD26 antigen,so as to provide useful help and further study.Methods:BALB/C mice were immunized 3 times with KD26 antigen of cysticercus cellulosae by SDS PAGE(mixed with Freund's incomplet adjuvant),and the spleen cells of immunized mice were fused with myeloma cell line SP2/0(in the ratio of 10∶1) in the HAT medium containing 50% PEG(4 000 MW),then incubated,selected and cloned.Numerical analysis on chromosome was performed by using metephase technique.The type and titer of the McAb was assayed by agar double diffusion technique and ELISA methods.Results:After being selected and cloned 3 times by using routine limited dilution method,one hybridoma cell line XH was generated,which contained 96 chromosomes and could steadily secrete specific McAb.The McAb belonged to IgG1 type.The titer was 1∶1 000.The McAb had no cross reactions with the antigens of Schistosome japonicum,Toxoplasma gondii and Echinococcus granulosus .Conclusions:By the hybridoma technique,we have established the hybridoma cell line which can secrete specific and high titers McAb against cysticercus cellulosae KD26 antigen.

目的 :建立针对猪囊尾蚴KD2 6抗原的单克隆杂交瘤细胞株 ,生产相应的单克隆抗体 ,用于囊虫病免疫保护机制的基础研究和囊虫病临床免疫诊断的应用。方法 :用SDS PAGE电泳 ,分离纯化制备猪囊尾蚴KD2 6抗原免疫 6周龄BALB/C小鼠 3次 ,无菌取出脾细胞与小鼠骨髓瘤细胞SP2 /O(比率 1 0∶1 ) ,在 5 0 %PEG(4 0 0 0MW )作用下进行细胞融合 ,随后在HAT培养液中进行选择性培养、筛选和克隆 ;常规的中期染色体法分析染色体 ,单克隆抗体鉴定采用免疫双扩散法测定其免疫球蛋白亚类 ,采用ELISA法测定单抗效价并作特异性鉴定。结果 :用常规的有限稀释法对杂交瘤细胞进行了 3次筛选和克隆化后 ,获得了 1株杂交瘤XH ,染色体数为 96 ,能稳定分泌抗猪囊尾蚴KD2 6单抗 ,单抗类型为IgG1 ,抗体滴度为 1∶1 0 0 0 ,其与血吸虫、弓形虫、细粒棘球绦虫抗原均不发生交叉反应。结论 :应用杂交瘤技术获得了 1株能稳定分泌高滴度抗猪囊尾蚴KD2 6抗原的单克隆抗体细胞株

With its recent development, radioimmunoimaging (RII) technique has been used not only for early diagnosing tumor but also for other diseases. The antibody antihalf-antigen and antigenic can be mass-produced by gene recombination and used in RII for clinical immunologic diagnosis and scientific researches. It is not only used for determining focal, size, range and location, but also for early detecting the primary lesion and showing the histological and pathological of lesions.

随着放射免疫显像应用技术的进展,放射免疫显像除用于肿瘤的早期诊断外,在其他疾病中的应用也日趋增多。利用基因重组技术大量生产的任何一种相应半抗原和抗原的抗体片段,与RII技术联合,已应用于临床免疫诊断和科学研究。它不仅能确定病灶的大小、范围、位置,还有助于早期发现原发病灶,提示病变组织学和病理学的变化等。

 
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