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酶活水平
相关语句
  enzyme activity level
     The PAL enzyme activity level can be used as a criterion for identifying resistant lines.
     苯丙氨酸解氨酶(PAL)酶活水平可用作抗性系的鉴定指标。
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  “酶活水平”译为未确定词的双语例句
     Whereas lignin peroxidase was detectable only in a narrow range of nitrogen source concentration (0.8~1.8 mmol/L), manganese peroxidase reached considerable levels over a broad range (0.4~2.0 mmol/L).
     LiP只在很窄的氮源浓度范围内(0.8~1.8 mmol/L)才能测到,而MnP在较宽浓度范围内(0.4~1.8 mmol/L)表现出较高的酶活水平.
短句来源
     The 3 g/dL initial starch concentration was proper for MTG fermentation. DCW and MTG activity could reach 25.1 g/L and 2.94 U/mL respectively.
     初始淀粉质量浓度以 3g/dL较适宜 ,其DCW和MTG酶活最高 ,DCW为 2 5.1g/L ,酶活水平达 2 .94U /mL ;
短句来源
     mobilis CP4. The enzymeactivity of talB using pZB31 as vector(0.051 U/mg total cell protein) is higher thanthat using pZB1(0.035 U/mg total cell protein).
     mobilis CP4中表达E. coli K-12的talB基因,酶活水平达0.051 U/mg蛋白,高于以pZB1为载体的相同基因的表达水平(为0.035 U/mg蛋白)。
短句来源
     Under the circumstances of 0.3 kg/cm pressure, 1:0.5v/v/m air flow, defined temperature and other controlled conditions, the level of penicillin-G-acylase activity has been reached 342U/100ml(NIPAB method), which is 1.8 fold higher in comparison with the shaking flask fermentation.
     在罐压0.3kg/cm2,通气比1:0.5及恒温条件下,借助上述手段,最高酶活水平已达342u/100ml(NIPAB法),比摇瓶发酵酶活提高1.8倍。
短句来源
     Conditions of fermentation is as following:pH5.0, inoculation 10%, bottle volume 50/500,temperature 28℃,fennentation time 48h. Under optimum medium and optimum fennentation conditions the enzyme production level was 340UI/mL.
     培养条件为:pH5.0,接种量10%,装瓶量50/500,发酵温度28℃,发酵时间48h, 酶活水平为340UT/mL.
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  相似匹配句对
     C were measured by the method of radioimmunology.
     C的水平
短句来源
     Preliminary probe into the influential factors of cellulase activity
     纤维素的影响因子初探
短句来源
     semitecum was capable to produce some higher Cellulase activity than R.
     stolonifer产的高。
短句来源
     The PAL enzyme activity level can be used as a criterion for identifying resistant lines.
     苯丙氨酸解氨(PAL)水平可用作抗性系的鉴定指标。
短句来源
     Chymotropsin activity was not different between the level of 2 percent and the level of 4 percen(tP>0.05).
     4%水平组的与对照组差异不显著(P>0.05)。
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  enzyme activity level
The stay of the scorpionfish under hypoxic conditions (15% saturation), which also is a stress for the fish, also produced a decrease of the enzyme activity level in erythrocytes by 62 % (p >amp;lt; 0.001).
      
enzyme activity level, stimulation of some metabolic pathways and stimulation of the normal liver recovery after partial hepatectomy).
      
The secretion yield of recombinant appA protein was accumulated to approximately 4.2 mg·mL-1, and the enzyme activity level reached 15,000 U·mL-1, which is higher than any previous reports.
      
There is no significant influence of sex and developmental time on the enzyme activity level, which appears in contrast to be greatly influenced by food (wheat or sorghum).
      
Each species exhibited significant changes in at least one enzyme activity level.
      
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Subcellular fractions of the blue-green alga were prepared through the treatment with lysozyme and sonication, followed by differential centrifugation and detergent solubilization. The proteolytic activities of the subcellular fractions were determined at 280 nm based on the reaction of α-casein. The results show that there are proteolytic activities in the soluble fraction, outer membrane, intracytoplasmic membrane, and periplasmic space. The activity of the soluble protease of heterocyst was 4—5 times higher...

Subcellular fractions of the blue-green alga were prepared through the treatment with lysozyme and sonication, followed by differential centrifugation and detergent solubilization. The proteolytic activities of the subcellular fractions were determined at 280 nm based on the reaction of α-casein. The results show that there are proteolytic activities in the soluble fraction, outer membrane, intracytoplasmic membrane, and periplasmic space. The activity of the soluble protease of heterocyst was 4—5 times higher than that of the vegetative cell.The properties of the soluble protease of vegetative cell grown in combined nitrogen-free medium were also investigated. The enzyme has an optimum reactive temperature around 50—55℃, and its activity decreased rapidly at 65℃. With the presence of calcium ion, the emzyme is stable at 60℃. Preincubation of the soluble protease at 60℃ for 10 min. without calcium ion resulted in a loss of more than 80% of the original activity. The activity of the enzyme was also found to be optimal at pH 8 to 10. Moreover the levels of the proteolytic activities of the vegetative cells were markedly affected by the pH of the culture medium upon nitrogen starvation. Alkaline condition greatly enhanced the activities.Activities of proteases were found to be inhibited completely by 1 m mol/I o-phenanthrolin but slightly by 1 m mol/I EDTA. Inhibition caused by PMSF was also observed but it varied with the duration of nitrogen starvation of the cells that produced the enzyme.

应用溶菌酶处理,超声破碎细胞,通过差异离心和解离剂处理,将细胞的不同部分分开。以水解α-酪蛋白反应检测蛋白水解酶活性,结果表明,在细胞的可溶部分、内细胞质膜、外膜及胞浆周围区均存在蛋白水解酶活性。异形胞可溶部分的蛋白酶活比营养细胞可溶的蛋白酶活高4—5倍。在固氮条件下,营养细胞可溶性蛋白酶反应最适温度为50—55℃,65℃时,酶活迅速下降。有Ca~(2+)5mmol/l时,蛋白酶在60℃稳定,无Ca~(2+)存在下,酶液在60℃预处理10分钟,酶活性丧失80%以上。酶反应的最适pH为8—10。而且氮饥饿之后,培养基的pH值对细胞蛋白酶活水平有明显影响,氮饥饿24小时内,蛋白酶活迅速增加,但在碱性条件下,酶活水平比在中性条件下要高得多。邻菲啰啉对蛋白酶活性有严重的抑制,EDTA仅有轻微抑制,而PMSF对细胞蛋白酶活的抑制作用,与细胞氮饥饿时间有关。

Auricularia polytricha and A.auricula were separately cultivated on the medium which consisted of sugarcane bagasse mainly.The contents of main chemical constituents and the activities of some enzymes related to the degradation of the substrates in the cultures were determined at different stages during the fungal growth. There were difference in capability to utilize the substrates between the fungi.At the late stage of the cultivation,74% and 43% of cellulose,78% and 61% of hemicellulose,and 60% and 35% of...

Auricularia polytricha and A.auricula were separately cultivated on the medium which consisted of sugarcane bagasse mainly.The contents of main chemical constituents and the activities of some enzymes related to the degradation of the substrates in the cultures were determined at different stages during the fungal growth. There were difference in capability to utilize the substrates between the fungi.At the late stage of the cultivation,74% and 43% of cellulose,78% and 61% of hemicellulose,and 60% and 35% of lignin were decomposed by A.polytricha and A.auricula respectively.Both the fungi,however,showed with a similar feature to consume the substrates by stages,i.e.the consumption rates in the fruitbody formation were more rapid than that in the vegetative growth.The activities of cellulase and hemicellulase were changed by stages too.In some extent there existed the positive correlation between the degradation rates of the substrates and the activity levels of the enzymes.Peroxidase and polyphenolase also assayed and the roles of the enzymes were discussed briefly.

实验以甘蔗渣加少量麦麸栽培毛木耳和光木耳,在栽培的不同阶段分析测定培养物的的主要化学组成成分和有关酶活性的变化。结果表明,两种木耳利用栽培底物的能力有明显差别;在栽培后期,毛木耳和光木耳分别消耗纤维素为74%和43%,半纤维素为78%和61%,木质素寿60%和35%。不过它们对这些底物的消耗又具有相似的阶段性变化的特点,即在子实体形成期的消耗速率都比菌丝生长期快。培养物的纤维素酶和半纤维素酶的活性也有阶段性变化特点。底物降解速率的变化与酶活水平的变化之间有一定对应关系,其中尤以毛木耳较明显。实验还分析培养物的过氧化物酶和多酚氧化酶的活性变化,简要地讨论了它们在栽培底物降解中的作用。

By complex mutagenesis of Asp. usamii B_1 with N(?)G, UV—raysand LiCl, a black-spore mutant strain, B_(1-12), was selected. The strain is Suitable for liquid culturatation with a shorter fermentation circle and optimum pH shift to a lower pH value. The activity of exo-PG increases while the activity of glucoamylase decreases. But the stability of pectic enzymes at 60℃ decreases. The mutant strain is superior to B_1 in clarifying several kinds of fruit juices.

B_(1—12)是由Asp·usamii B_1经NTG、UV、LiCl复合处理选育到的一株呈黑色的孢子是适于液体培养的高酶活果胶酶菌株。具有发酵周期短、易过滤等痔点,酒樁沉淀的酶制剂与B_1比较,酶作用的最适pH分别为4.0和4.5,最适温度均力50℃,但在30℃时,B_1仍保持较高酶活水平的稳定性。B_(1—12)果胶酶组分ex—PG酶活高于B_1 0.5—1倍左右,endo—PG和PE变化不明显.两者都含有糖化酶、纤维素酶、蛋白酶、花青素酶等非果胶酶组分,其中糖化酶活性比B_1下降一倍.应用于苹果醋、苹果汁、草莓汁、樱桃汁的澄清.B_(1—12)优于B_1,透光率可达90%以上,但对枣汁的澄清效果却次于B_1。

 
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